Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 454,434 G→A intergenic (‑267/‑38) yajG ← / → bolA putative lipoprotein/stationary‑phase morphogene, transcriptional repressor for mreB, also regulator for dacA, dacC, and ampC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913454,4340GA100.0% 174.6 / NA 54intergenic (‑267/‑38)yajG/bolAputative lipoprotein/stationary‑phase morphogene, transcriptional repressor for mreB, also regulator for dacA, dacC, and ampC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/30);  total (24/30)

CTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGGATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCGTCACA  >  NC_000913/454304‑454559
                                                                                                                                  |                                                                                                                             
cagATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTTGTATTTAAAGGAATGGATGa                                                                                                                       <  2:771900/137‑1 (MQ=255)
cTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGa                                                                                                                       <  1:1101616/139‑1 (MQ=255)
cTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTGAAAGGAATGGATGa                                                                                                                       <  1:324108/139‑1 (MQ=255)
   aTGCGAGGCCTGTGTTTCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACAt                                                                                                                    >  2:354994/1‑139 (MQ=255)
         ggCCTGTGTTTCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGc                                                                                                              <  2:715128/139‑1 (MQ=255)
             tgtgTTTCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTg                                                                                                          >  1:1034327/1‑139 (MQ=255)
             tgtgTTTCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTg                                                                                                          <  1:760758/139‑1 (MQ=255)
              gtgtTTCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGt                                                                                                         <  2:359119/139‑1 (MQ=255)
                  ttCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCgga                                                                                                     <  2:1034327/139‑1 (MQ=255)
                   tCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCggag                                                                                                    <  1:1071615/139‑1 (MQ=255)
                   tCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCggag                                                                                                    <  1:433969/139‑1 (MQ=255)
                         tttAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGata                                                                                              <  2:305637/139‑1 (MQ=255)
                         tttAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGata                                                                                              >  2:923391/1‑139 (MQ=255)
                         tttAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGata                                                                                              >  1:684360/1‑139 (MQ=255)
                             aaTCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTc                                                                                          <  2:256834/139‑1 (MQ=255)
                                         tCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGt                                                                              <  1:904545/139‑1 (MQ=255)
                                          cATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGgaggag                                                                                                 <  2:475090/119‑1 (MQ=255)
                                          cATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGgaggag                                                                                                 >  1:475090/1‑119 (MQ=255)
                                             aCGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGc                                                                          >  2:395156/1‑139 (MQ=255)
                                                 ttGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGAt                                                                      >  2:727418/1‑139 (MQ=255)
                                                  tGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTATCGGAGGAGATATTTCATGATGATACGTGAGCGGATa                                                                     >  1:1211652/1‑139 (MQ=255)
                                                    aCGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATaga                                                                   >  2:439744/1‑139 (MQ=255)
                                                              cAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTc                                                                                          >  1:1219034/1‑106 (MQ=255)
                                                              cAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTc                                                                                          <  2:1219034/106‑1 (MQ=255)
                                                                ggACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCa                                                                                                                >  2:532722/1‑82 (MQ=255)
                                                                ggACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCa                                                                                                                <  1:532722/82‑1 (MQ=255)
                                                                ggACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATagaaga                                                                >  2:741603/1‑130 (MQ=255)
                                                                ggACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATagaaga                                                                <  1:741603/130‑1 (MQ=255)
                                                                 gACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACAtctc                                                                                                                 <  2:74630/80‑1 (MQ=255)
                                                                 gACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACAtctc                                                                                                                 >  1:74630/1‑80 (MQ=255)
                                                                  aCGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAggg                                                     >  2:561130/1‑139 (MQ=255)
                                                                     aaaCCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGggcgg                                                  >  1:47534/1‑139 (MQ=255)
                                                                         cTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGtt                                              <  2:684360/139‑1 (MQ=255)
                                                                           aaaTATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTcc                                            <  1:354994/139‑1 (MQ=255)
                                                                                  tgttgtTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTg                                                                                                          >  2:627308/1‑70 (MQ=255)
                                                                                  tgttgtTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTg                                                                                                          <  1:627308/70‑1 (MQ=255)
                                                                                         aaGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGACGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTc                              >  2:385299/1‑139 (MQ=255)
                                                                                          aGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCg                             <  1:561130/139‑1 (MQ=255)
                                                                                               cAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGAt                                                                      <  2:418888/93‑1 (MQ=255)
                                                                                               cAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGAt                                                                      >  1:418888/1‑93 (MQ=255)
                                                                                               cAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAAt                                                           <  1:227929/104‑1 (MQ=255)
                                                                                               cAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAAt                                                           >  2:227929/1‑104 (MQ=255)
                                                                                                aaTGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAagtag                       <  1:727418/139‑1 (MQ=255)
                                                                                                 aTGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGtt                                              >  2:610422/1‑115 (MQ=255)
                                                                                                 aTGGAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGtt                                              <  1:610422/115‑1 (MQ=255)
                                                                                                   ggAAACGGTAAAAGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTgg                    <  2:168950/139‑1 (MQ=255)
                                                                                                             aaaGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGgaga                                                                                                >  1:123118/1‑53 (MQ=255)
                                                                                                             aaaGCGGCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGgaga                                                                                                <  2:123118/53‑1 (MQ=255)
                                                                                                                gctgCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCg       <  2:1211652/136‑1 (MQ=255)
                                                                                                                   gCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTcc                                            <  1:936146/99‑1 (MQ=255)
                                                                                                                   gCTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTcc                                            >  2:936146/1‑99 (MQ=255)
                                                                                                                    cTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGggcg                                                   <  2:597019/91‑1 (MQ=255)
                                                                                                                    cTAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGggcg                                                   >  1:597019/1‑91 (MQ=255)
                                                                                                                     tAGTATTTAAAGGAATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCGTcaca  >  1:267086/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                             
CTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTAAACTTCATACGCTTGACGGAAAAACCAGGACGAAACCTAAATATTTGTTGTTAAGCTGCAATGGAAACGGTAAAAGCGGCTAGTATTTAAAGGGATGGATGACATCTCAGCGTTGTCGGAGGAGATATTTCATGATGATACGTGAGCGGATAGAAGAAAAATTAAGGGCGGCGTTCCAACCCGTATTCCTCGAAGTAGTGGATGAAAGCTATCGTCACA  >  NC_000913/454304‑454559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: