Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,200,454 (A)8→7 coding (176/3633 nt) yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,200,4470A.100.0% 197.5 / NA 49coding (169/3633 nt)yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (27/22);  total (27/22)

CCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCG  >  NC_000913/2200314‑2200581
                                                                                                                                     |                                                                                                                                      
ccAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaa                                                                                                                                   >  1:713156/1‑139 (MQ=255)
   aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca                                                                                                                                <  1:361652/139‑3 (MQ=255)
       gAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGGCATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGt                                                                                                                           <  1:1131070/139‑1 (MQ=255)
       gAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGt                                                                                                                           <  2:256104/139‑1 (MQ=255)
                     aaaaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATgaga                                                                                                             >  2:1307476/1‑139 (MQ=255)
                          gCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca                                                                                                                                >  1:1073913/1‑114 (MQ=255)
                          gCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca                                                                                                                                <  2:1073913/116‑3 (MQ=255)
                                    gcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGt                                                                                              >  1:94899/1‑139 (MQ=255)
                                                    tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCAc                                                                              >  1:1373077/1‑139 (MQ=255)
                                                     ggCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACg                                                                             <  2:94899/139‑1 (MQ=255)
                                                          ggCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAtt                                                                                                          <  1:12526/105‑1 (MQ=255)
                                                          ggCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAtt                                                                                                          >  2:12526/1‑105 (MQ=255)
                                                                     gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGa                                                             >  1:1068971/1‑139 (MQ=255)
                                                                     gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGa                                                             >  1:115356/1‑139 (MQ=255)
                                                                        cGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑‑AAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCgg                                                         >  2:1363569/1‑139 (MQ=255)
                                                                           tATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCg                                                       <  2:965308/139‑1 (MQ=255)
                                                                              cACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAac                                                    >  1:104799/1‑139 (MQ=255)
                                                                                cTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAacac                                                  <  1:608472/139‑1 (MQ=255)
                                                                                     aGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAAcc                                             >  1:1198922/1‑139 (MQ=255)
                                                                                       tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                                           >  2:235606/1‑139 (MQ=255)
                                                                                       tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                                           <  2:115356/139‑1 (MQ=255)
                                                                                        ctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTc                                          >  1:184140/1‑139 (MQ=255)
                                                                                        ctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTc                                          >  1:1186199/1‑139 (MQ=255)
                                                                                           aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                                       >  2:254296/1‑139 (MQ=255)
                                                                                            tCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                                      <  1:25093/139‑1 (MQ=255)
                                                                                                 aCATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc                                 <  2:1198922/139‑1 (MQ=255)
                                                                                                     tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg                             >  1:117182/1‑139 (MQ=255)
                                                                                                     tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg                             >  1:1467613/1‑139 (MQ=255)
                                                                                                       ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGt                                                                                                                           >  1:1295752/1‑43 (MQ=255)
                                                                                                       ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGt                                                                                                                           <  2:1295752/43‑1 (MQ=255)
                                                                                                       ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAAcaca                                                 >  1:1413557/1‑117 (MQ=255)
                                                                                                       ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAAcaca                                                 <  2:1413557/117‑1 (MQ=255)
                                                                                                       ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGAc                           >  2:915104/1‑139 (MQ=255)
                                                                                                           atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATg                       >  1:780138/1‑139 (MQ=255)
                                                                                                           atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGACATCAACTTGATCGGCGAACACAAACCCTCAATCACGGCCCGGACAATg                       >  2:352558/1‑139 (MQ=255)
                                                                                                             gaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATggc                     >  1:91764/1‑139 (MQ=255)
                                                                                                             gaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATggc                     >  2:834494/1‑139 (MQ=255)
                                                                                                               aTTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATggcgg                   >  1:936617/1‑139 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAGCATTGTCTGGc                                                                                                                      <  1:933854/38‑1 (MQ=25)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGc                                                                                                                      >  2:933854/1‑38 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                                           >  1:1278599/1‑81 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                                           <  2:1278599/81‑1 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:477848/139‑1 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:713156/139‑1 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:254296/139‑1 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:184140/139‑1 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:1467613/139‑1 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:1373077/139‑1 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:117182/139‑1 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:915104/139‑1 (MQ=255)
                                                                                                                 tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 >  1:68784/1‑139 (MQ=255)
                                                                                                                 ggcaaCAAGAAGCTGAGAATAAAAAAACAGTCTCTGGCGTTATGAGAAGTTATCGCGGGAAGGGCAGTCCTCCATGACGCAGGCCATCAGCGTAATCGACGAACACTAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:352558/135‑1 (MQ=255)
                                                                                                                    tACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAacac                                                  <  2:104591/103‑1 (MQ=255)
                                                                                                                    tACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAacac                                                  >  1:104591/1‑103 (MQ=255)
                                                                                                                                agaagaAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATcgcg  >  1:1277916/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                      
CCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCG  >  NC_000913/2200314‑2200581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: