Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,8810C.94.0% 247.9 / 9.5 67intergenic (+235/‑131)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base . (24/39);  total (26/41)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

TTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAA  >  NC_000913/2462757‑2462999
                                                                                                                            |                                                                                                                      
ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTAt                                                                                                         <  2:884970/139‑1 (MQ=255)
ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTAt                                                                                                         <  2:1508528/139‑1 (MQ=255)
    cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                     <  1:320025/139‑1 (MQ=255)
      gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                   >  2:1500626/1‑139 (MQ=255)
        ttGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCt                                                                                                 >  2:1279696/1‑139 (MQ=255)
           cccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCAt                                                                                              <  2:1122610/139‑1 (MQ=255)
              tttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                                                                                                     >  1:190352/1‑114 (MQ=255)
              tttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                                                                                                     <  2:190352/114‑1 (MQ=255)
                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                        <  1:130587/139‑1 (MQ=255)
                                   ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCt                                                                      <  1:639297/139‑1 (MQ=255)
                                        gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCAGTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATAGACTCAAATTAAAGATAGGTTCtaaata                                                                 >  1:925168/1‑139 (MQ=255)
                                         aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataa                                                                >  1:1611531/1‑139 (MQ=255)
                                          cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataaa                                                               >  1:420516/1‑139 (MQ=255)
                                            aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                             >  1:791594/1‑139 (MQ=255)
                                              ccagAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGACTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCTCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                           <  1:1108935/136‑1 (MQ=255)
                                              ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                           <  2:782103/139‑1 (MQ=255)
                                                   aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                        <  2:955479/121‑1 (MQ=255)
                                                   aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                        >  1:955479/1‑121 (MQ=255)
                                                        aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCTAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                 <  1:976003/139‑1 (MQ=255)
                                                        aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                 <  1:1279696/139‑1 (MQ=255)
                                                        aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                 <  2:1121730/139‑1 (MQ=255)
                                                        aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                 <  1:77423/139‑1 (MQ=255)
                                                         aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCa                                                                                               <  2:686739/92‑1 (MQ=255)
                                                         aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCa                                                                                               >  1:686739/1‑92 (MQ=255)
                                                         aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                                                <  1:1551098/139‑1 (MQ=255)
                                                         aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                                                >  1:1526173/1‑139 (MQ=255)
                                                           gCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                                              <  2:925168/139‑1 (MQ=255)
                                                            cATCTTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                             >  2:1533093/1‑139 (MQ=255)
                                                            cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccct                                                                                                                <  2:549698/73‑2 (MQ=255)
                                                            cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccct                                                                                                                >  1:549698/1‑72 (MQ=255)
                                                              cagtttCAGCCGTTCTGCCCGTCCTTCTCAAGGAGTTTACTTTTCTCCATTTCCGGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTc                                            <  2:1488992/136‑1 (MQ=255)
                                                               atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTc                                                                                                                        >  2:1406316/1‑62 (MQ=255)
                                                               atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTc                                                                                                                        <  1:1406316/62‑1 (MQ=255)
                                                                    cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                     <  2:420516/139‑1 (MQ=255)
                                                                     aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             <  1:1060287/98‑1 (MQ=255)
                                                                     aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                     <  1:1522543/138‑1 (MQ=255)
                                                                     aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                     >  2:1522543/1‑138 (MQ=255)
                                                                     aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                    <  1:1500626/139‑1 (MQ=255)
                                                                     aGGCGTTCTGCTCGTCCTGCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             >  2:1060287/1‑98 (MQ=255)
                                                                      ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTTTTTCAtt                                   <  1:1053857/139‑1 (MQ=255)
                                                                      ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                   <  2:1611531/139‑1 (MQ=255)
                                                                      ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                   <  2:1526173/139‑1 (MQ=255)
                                                                      ggCGTTCTGCTCGTCCTTCTCAAAGAGGTTACTTTTCTGCATTTCCAGGATACTCCCCCCCTTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                   <  1:347459/139‑1 (MQ=255)
                                                                       gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                     <  1:776435/72‑1 (MQ=255)
                                                                       gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                     >  2:776435/1‑72 (MQ=255)
                                                                         gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATa                                                                                             <  2:164765/78‑1 (MQ=255)
                                                                         gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATa                                                                                             >  1:164765/1‑78 (MQ=255)
                                                                         gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAACGAGCGTTTTTTGATAGTCTATTTCATTAgg                                <  2:560325/139‑1 (MQ=255)
                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                   >  1:1587544/1‑56 (MQ=255)
                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                   >  1:1034743/1‑56 (MQ=255)
                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                   <  2:1587544/56‑1 (MQ=255)
                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                   <  2:1034743/56‑1 (MQ=255)
                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                     <  2:921721/69‑1 (MQ=255)
                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                     >  1:921721/1‑69 (MQ=255)
                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTGTTTCa                                     >  2:1048457/1‑133 (MQ=255)
                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                     <  1:1048457/133‑1 (MQ=255)
                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGt                               <  2:791594/139‑1 (MQ=255)
                                                                            cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                   >  1:1229890/1‑54 (MQ=255)
                                                                            cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                   <  2:1229890/54‑1 (MQ=255)
                                                                            cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACCCTCAAATTAAAGATAGGTTCTAAATAAATGAGGGTTTTTTTATTGTTTATTTTATTAgctg                              >  1:1488992/1‑136 (MQ=255)
                                                                            cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                              >  2:919114/1‑138 (MQ=255)
                                                                            cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                              <  1:919114/138‑1 (MQ=255)
                                                                                       tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                >  2:1027644/1‑126 (MQ=255)
                                                                                       tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                <  1:1027644/126‑1 (MQ=255)
                                                                                       tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                >  2:1406187/1‑125 (MQ=255)
                                                                                       tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                <  1:1406187/125‑1 (MQ=255)
                                                                                         tcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             <  2:626895/78‑1 (MQ=255)
                                                                                         tcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             >  1:626895/1‑78 (MQ=255)
                                                                                             agagTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                          >  1:665029/1‑109 (MQ=255)
                                                                                             agagTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                          <  2:665029/109‑1 (MQ=255)
                                                                                                  ttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                     >  2:277008/1‑45 (MQ=255)
                                                                                                  ttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                     <  1:277008/45‑1 (MQ=255)
                                                                                                       tttCTGCATTTCCATGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTTAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa  <  1:1533093/139‑1 (MQ=255)
                                                                                                           tGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                   >  2:216757/1‑38 (MQ=255)
                                                                                                           tGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                   <  1:216757/38‑1 (MQ=255)
                                                                                                           tGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                          >  1:1174362/1‑63 (MQ=255)
                                                                                                           tGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                          <  2:1174362/63‑1 (MQ=255)
                                                                                                                  ccAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             >  1:1228015/1‑53 (MQ=255)
                                                                                                                  ccAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             <  2:1228015/53‑1 (MQ=255)
                                                                                                                            |                                                                                                                      
TTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAA  >  NC_000913/2462757‑2462999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: