Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 247,466 Δ1 bp intergenic (+5/‑171) yafL → / → rayT putative lipoprotein and C40 family peptidase/RAYT REP element‑mobilizing transposase, TnpA(REP)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913247,4650G.100.0% 244.6 / NA 63intergenic (+4/‑172)yafL/rayTputative lipoprotein and C40 family peptidase/RAYT REP element‑mobilizing transposase, TnpA(REP)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (33/30);  total (33/30)

TTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NC_000913/247329‑247594
                                                                                                                                        |                                                                                                                                 
tttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACgat                                                                                                                                 <  1:527344/139‑3 (MQ=255)
  tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACgataa                                                                                                                               <  1:1183716/139‑5 (MQ=255)
      cGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCg                                                                                                                          >  1:344707/1‑139 (MQ=255)
        aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAgg                                                                                                                            >  1:488260/1‑135 (MQ=255)
        aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAgg                                                                                                                            <  2:488260/135‑1 (MQ=255)
        aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGtt                                                                                                                        >  1:1180156/1‑139 (MQ=255)
        aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGtt                                                                                                                        >  2:736774/1‑139 (MQ=255)
        aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGtt                                                                                                                        >  2:69665/1‑139 (MQ=255)
           ggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGTCGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTAc                                                                                                                     >  1:230824/1‑139 (MQ=255)
                     cGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTAcgccg                                                                                                                 >  1:44813/1‑133 (MQ=255)
                     cGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTAcgccg                                                                                                                 <  2:44813/133‑1 (MQ=255)
                      gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:736774/139‑1 (MQ=255)
                      gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  2:344707/139‑1 (MQ=255)
                        gTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                        <  1:69665/139‑1 (MQ=255)
                          cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                                      >  1:480660/1‑139 (MQ=255)
                          cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                                      >  1:1146776/1‑139 (MQ=255)
                                       cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                         <  2:1004668/139‑1 (MQ=255)
                                           aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa                                                                                     >  1:1394971/1‑139 (MQ=255)
                                           aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa                                                                                     >  1:1369437/1‑139 (MQ=255)
                                                tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGa                                                                                >  2:708199/1‑139 (MQ=255)
                                                       aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:408722/139‑1 (MQ=255)
                                                       aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:1104128/139‑1 (MQ=255)
                                                       aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:708199/139‑1 (MQ=255)
                                                           gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                     <  1:1517590/139‑1 (MQ=255)
                                                             ttAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCt                                                                   >  1:1030446/1‑139 (MQ=255)
                                                              tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                     <  2:792717/136‑1 (MQ=255)
                                                              tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                     >  1:792717/1‑136 (MQ=255)
                                                               aGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCTGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                                                 <  1:781499/139‑1 (MQ=255)
                                                                 ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  2:1369437/139‑1 (MQ=255)
                                                                 ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  2:1180156/139‑1 (MQ=255)
                                                                   gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  1:1099120/137‑1 (MQ=255)
                                                                   gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               >  2:1099120/1‑137 (MQ=255)
                                                                          tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                      >  1:1568774/1‑139 (MQ=255)
                                                                           cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                     >  1:629037/1‑139 (MQ=255)
                                                                            tGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                    <  2:1030446/139‑1 (MQ=255)
                                                                                 ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:66435/113‑1 (MQ=255)
                                                                                 ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         >  1:66435/1‑113 (MQ=255)
                                                                                  gACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgcg                                              >  2:1083303/1‑139 (MQ=255)
                                                                                   aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCa                                                                                                               <  2:1401768/73‑1 (MQ=38)
                                                                                   aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCa                                                                                                               >  1:1401768/1‑73 (MQ=38)
                                                                                   aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCa                                                                                                               <  2:1000650/73‑1 (MQ=38)
                                                                                   aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCa                                                                                                               >  1:1000650/1‑73 (MQ=38)
                                                                                   aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                        >  2:996194/1‑96 (MQ=255)
                                                                                   aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                        <  1:996194/96‑1 (MQ=255)
                                                                                             gggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGa                                   >  1:540050/1‑139 (MQ=255)
                                                                                                  cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                         <  2:815674/80‑1 (MQ=38)
                                                                                                  cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                         >  1:815674/1‑80 (MQ=37)
                                                                                                  cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                     <  1:410266/100‑1 (MQ=255)
                                                                                                  cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                     >  2:410266/1‑100 (MQ=255)
                                                                                                  cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                              >  1:592795/1‑139 (MQ=255)
                                                                                                       aGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:1394971/139‑1 (MQ=255)
                                                                                                                 cGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATaaaa               >  2:795283/1‑139 (MQ=255)
                                                                                                                   gAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                       <  2:362940/49‑1 (MQ=17)
                                                                                                                   gAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                       >  1:362940/1‑49 (MQ=17)
                                                                                                                   gAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGc                                                                                  >  1:210631/1‑70 (MQ=21)
                                                                                                                   gAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGc                                                                                  <  2:210631/70‑1 (MQ=21)
                                                                                                                   gAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                          <  2:380548/78‑1 (MQ=21)
                                                                                                                   gAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                          >  1:380548/1‑78 (MQ=21)
                                                                                                                   gAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                    <  1:1370743/116‑1 (MQ=255)
                                                                                                                   gAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                    >  2:1370743/1‑116 (MQ=255)
                                                                                                                    aaGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         >  1:780255/1‑78 (MQ=21)
                                                                                                                    aaGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:780255/78‑1 (MQ=21)
                                                                                                                         aCGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCa       >  2:72188/1‑139 (MQ=255)
                                                                                                                            aTTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTg    <  2:1029249/139‑1 (MQ=255)
                                                                                                                              tttGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc  <  2:592795/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
TTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NC_000913/247329‑247594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: