Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,860,426 Δ1 bp intergenic (+10/+41) pphB → / ← ygbI serine/threonine‑specific protein phosphatase 2/DeoR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,860,4250T.97.3% 283.8 / 2.1 74intergenic (+9/+42)pphB/ygbIserine/threonine‑specific protein phosphatase 2/DeoR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base . (36/36);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-01

AATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGAT  >  NC_000913/2860287‑2860558
                                                                                                                                          |                                                                                                                                     
aaTAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                                       <  2:386280/139‑1 (MQ=255)
        gcgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtgtg                                                                                                                              >  1:738771/1‑139 (MQ=255)
        gagcTGAATATTTTATAGTTGGACATCTGATGCATGATAACATTCAGACCATCCATAACCAGATCTATATTGTTAACGGATCGGCGAACAGCGGGCGGCTGAAATTTTCTAAAATACAGTAATCTCATGCTCTTCtgtg                                                                                                                              <  1:1365815/137‑1 (MQ=255)
                       tatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                               <  2:88793/139‑1 (MQ=255)
                            ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                                                >  1:904121/1‑133 (MQ=255)
                            ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                                                <  2:904121/133‑1 (MQ=255)
                            ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGTTCTTCTGTGAAGCATGAGTAAcc                                                                                                                >  1:1271051/1‑133 (MQ=255)
                            ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGTTCTTCTGTGAAGCATGAGTAAcc                                                                                                                <  2:1271051/133‑1 (MQ=255)
                             gACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGctc                                                                                                                                      <  1:629933/111‑2 (MQ=255)
                             gACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGctc                                                                                                                                      >  2:629933/1‑110 (MQ=255)
                                 tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                                     >  1:607163/1‑139 (MQ=255)
                                 tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                                     >  2:889512/1‑139 (MQ=255)
                                        tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                                     <  1:945968/132‑1 (MQ=255)
                                        tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                                     >  2:945968/1‑132 (MQ=255)
                                        tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCa                                                                                              >  1:622575/1‑139 (MQ=255)
                                            aTAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGAGCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCattat                                                                                          >  1:1139257/1‑139 (MQ=255)
                                             tAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGa                                                                                                                             <  1:1301068/103‑1 (MQ=255)
                                             tAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGa                                                                                                                             >  2:1301068/1‑103 (MQ=255)
                                                  ttCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCAttt                                                                                    >  2:1281649/1‑139 (MQ=255)
                                                  ttCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCAttt                                                                                    >  2:352348/1‑139 (MQ=255)
                                                       aCGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGc                                                                               <  2:1238510/139‑1 (MQ=255)
                                                       aCGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGc                                                                               <  1:747623/139‑1 (MQ=255)
                                                         tataagagacaggATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                             <  2:997898/127‑1 (MQ=255)
                                                          ttCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAc                                                                            >  2:295759/1‑139 (MQ=255)
                                                           tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                             <  2:1380521/137‑1 (MQ=255)
                                                           tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                             >  1:1380521/1‑137 (MQ=255)
                                                            cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                             <  2:1428423/136‑1 (MQ=255)
                                                            cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                             >  1:1428423/1‑136 (MQ=255)
                                                            cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                          >  2:1311221/1‑139 (MQ=255)
                                                            cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                          <  1:1311221/139‑1 (MQ=255)
                                                            cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                          >  1:1218459/1‑139 (MQ=255)
                                                                      aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                                     >  1:497271/1‑86 (MQ=255)
                                                                      aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                                     <  2:497271/86‑1 (MQ=255)
                                                                          atatTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATcc                                                            <  1:352348/139‑1 (MQ=255)
                                                                           tatTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATATAATAAAGTAATCTCTTGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                                   <  1:1060951/99‑1 (MQ=255)
                                                                           tatTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAgg                                                                                                 >  2:1531155/1‑101 (MQ=255)
                                                                           tatTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAgg                                                                                                 <  1:1531155/101‑1 (MQ=255)
                                                                           tatTGATACCGGATCGCCAAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                                   >  2:1060951/1‑99 (MQ=255)
                                                                                aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                               <  2:328256/82‑1 (MQ=255)
                                                                                aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                               >  1:328256/1‑82 (MQ=255)
                                                                                aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  2:511388/1‑139 (MQ=255)
                                                                                aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  1:902965/1‑139 (MQ=255)
                                                                                aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  2:649340/1‑139 (MQ=255)
                                                                                aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  1:860809/1‑139 (MQ=255)
                                                                                aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTAATGCTCTTCTGTGAAGCATGAGTAACACGGTGTTATTGCAGGACGATTTTCATTTTTCGCTACCAGCAAagagctgtctcttata                                                      >  1:997898/1‑127 (MQ=255)
                                                                                  aCCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAg                                                                         >  2:1386431/1‑118 (MQ=255)
                                                                                  aCCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAg                                                                         <  1:1386431/118‑1 (MQ=255)
                                                                                  aCCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCAc                                                    <  2:860809/139‑1 (MQ=255)
                                                                                    cGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCa                                                  >  1:268746/1‑139 (MQ=255)
                                                                                      gATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAg                                                                                                                           >  2:132411/1‑64 (MQ=255)
                                                                                      gATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAg                                                                                                                           <  1:132411/64‑1 (MQ=255)
                                                                                         cGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgcg                                             <  2:622575/139‑1 (MQ=255)
                                                                                                cAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTgg                                      <  2:738771/139‑1 (MQ=255)
                                                                                                cAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTgg                                      <  1:889512/139‑1 (MQ=255)
                                                                                                   cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtgtg                                                                                                                              <  2:824561/48‑1 (MQ=255)
                                                                                                   cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtgtg                                                                                                                              >  1:824561/1‑48 (MQ=255)
                                                                                                   cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtg                                                                                                                                <  1:544739/46‑1 (MQ=255)
                                                                                                   cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtg                                                                                                                                >  2:544739/1‑46 (MQ=255)
                                                                                                   cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCa                                    <  1:258246/138‑1 (MQ=255)
                                                                                                   cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCa                                    >  2:258246/1‑138 (MQ=255)
                                                                                                     ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                                     <  2:1595493/55‑1 (MQ=255)
                                                                                                     ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                                     >  1:1595493/1‑55 (MQ=255)
                                                                                                           tGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGc                           <  1:622512/139‑1 (MQ=255)
                                                                                                                   tataAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtc                   <  2:268746/139‑1 (MQ=255)
                                                                                                                     taaaataaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTTGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCTACTGGCACTCTCCGGCAAACcgtcgt                 <  1:226043/139‑1 (MQ=255)
                                                                                                                     taaaataaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt                 <  2:1304007/139‑1 (MQ=255)
                                                                                                                     taaaataaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCGCCGGCAAACcgtcgt                 <  1:295759/139‑1 (MQ=255)
                                                                                                                         ataaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGt             >  2:693491/1‑139 (MQ=255)
                                                                                                                          taaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGc                                                                               <  1:857923/72‑1 (MQ=255)
                                                                                                                          taaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGc                                                                               >  2:857923/1‑72 (MQ=255)
                                                                                                                          taaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGta            <  2:1139257/139‑1 (MQ=255)
                                                                                                                          taaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGta            <  1:511388/139‑1 (MQ=255)
                                                                                                                             agtaatCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                               >  2:1183440/1‑37 (MQ=255)
                                                                                                                             agtaatCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                               <  1:1183440/37‑1 (MQ=255)
                                                                                                                             agtaatCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGGGTTATTGCAGGCCAATATTGATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCACCGGCAAACTGTCGTCTGtaata         >  1:1061739/1‑139 (MQ=255)
                                                                                                                               taatctcATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTaataat       <  2:1218459/136‑1 (MQ=255)
                                                                                                                                    tcATGCTCTTCTGTGAAGCATGATTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGAt  >  2:466653/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                     
AATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGAT  >  NC_000913/2860287‑2860558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: