Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 7,925 +A coding (35/1431 nt) yaaJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009137,9221.A97.0% 207.2 / ‑1.3 67coding (38/1431 nt)yaaJputative transporter
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base A (33/32);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCT  >  NC_000913/7784‑8055
                                                                                                                                           |                                                                                                                                      
gTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATcccc                                                                                                                                         >  2:1319298/1‑139 (MQ=255)
     aCCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaag                                                                                                                                  >  1:186703/1‑137 (MQ=255)
       cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                                <  1:531038/139‑5 (MQ=255)
       cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                                >  2:531038/1‑135 (MQ=255)
       cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                                <  1:1548199/139‑5 (MQ=255)
         ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                               >  2:121034/1‑139 (MQ=255)
         ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                               <  2:186703/139‑1 (MQ=255)
         ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                                >  1:968140/1‑133 (MQ=255)
         ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                                <  2:968140/137‑5 (MQ=255)
          cTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                              >  1:1004937/1‑139 (MQ=255)
                   gATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                               >  1:952918/1‑129 (MQ=255)
                   gATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                               <  2:952918/129‑1 (MQ=255)
                                   aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAg                                                                                                                   <  2:1234215/125‑1 (MQ=255)
                                   aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAg                                                                                                                   >  1:1234215/1‑125 (MQ=255)
                                   aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                     >  1:1557782/1‑139 (MQ=255)
                                   aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                     >  2:1529918/1‑139 (MQ=255)
                                   aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                     <  1:491874/139‑1 (MQ=255)
                                      aCTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcat                                                                                                  <  2:735863/139‑1 (MQ=255)
                                      aCTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcat                                                                                                  <  1:605894/139‑1 (MQ=255)
                                        ttttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgc                                                                                                >  1:613902/1‑139 (MQ=255)
                                          ttGCCAAACTGGTGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat                                                                                              >  2:1364403/1‑139 (MQ=255)
                                          ttGCCAAACTGGCGTATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAGAATCTGgcatgcat                                                                                              <  2:660814/139‑1 (MQ=255)
                                          ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat                                                                                              >  2:822886/1‑139 (MQ=255)
                                           tGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCata                                                                                             >  2:336040/1‑139 (MQ=255)
                                           tGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCata                                                                                             <  1:1319298/139‑1 (MQ=255)
                                              cAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAAACAACCACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAGAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATcc                                                                                          <  1:1529918/139‑1 (MQ=255)
                                                 aCTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCctc                                                                                       >  2:45277/1‑139 (MQ=255)
                                                               cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTcgc                                                                         >  2:548192/1‑139 (MQ=255)
                                                                 aaaCTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa                                                                       <  2:565501/139‑1 (MQ=255)
                                                                       cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACtt                                                                 >  2:374408/1‑139 (MQ=255)
                                                                         caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                               >  2:384068/1‑139 (MQ=255)
                                                                         caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                               <  1:384068/139‑1 (MQ=255)
                                                                         caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                               <  1:121034/139‑1 (MQ=255)
                                                                         caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAACAGGACGCTGTTAATGAAGGAGAACAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                               <  1:374408/139‑1 (MQ=255)
                                                                               cGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTgtgt                                                         <  2:1241305/139‑1 (MQ=255)
                                                                                ggTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTa                                                        <  2:231535/139‑1 (MQ=255)
                                                                                 gTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                               <  1:505574/99‑1 (MQ=255)
                                                                                 gTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                               >  2:505574/1‑99 (MQ=255)
                                                                                      aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGtt                                                  >  1:1009132/1‑139 (MQ=255)
                                                                                           tGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCaa                                             <  1:1364403/139‑1 (MQ=255)
                                                                                                   caacCTGCGCCGAAGAGCAGGTAAATCATTTCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCGTATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaa                                     <  2:1557782/139‑1 (MQ=255)
                                                                                                     acCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaaga                                   <  1:822886/139‑1 (MQ=255)
                                                                                                          cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                               >  1:1225154/1‑42 (MQ=255)
                                                                                                          cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                               <  2:1225154/42‑1 (MQ=255)
                                                                                                          cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                               >  2:780388/1‑42 (MQ=255)
                                                                                                          cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                               <  1:780388/42‑1 (MQ=255)
                                                                                                          cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCtgtg                                                          <  2:617777/111‑1 (MQ=255)
                                                                                                          cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCtgtg                                                          >  1:617777/1‑111 (MQ=255)
                                                                                                              gAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATAc                          >  1:806747/1‑139 (MQ=255)
                                                                                                               aaGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACg                         >  2:1266474/1‑139 (MQ=255)
                                                                                                                    cAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg                    <  2:1009132/139‑1 (MQ=255)
                                                                                                                    cAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg                    >  2:846172/1‑139 (MQ=255)
                                                                                                                    cAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg                    <  1:846172/139‑1 (MQ=255)
                                                                                                                         aaaTCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGt                >  1:47700/1‑138 (MQ=255)
                                                                                                                         aaaTCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGt                <  2:47700/138‑1 (MQ=255)
                                                                                                                              aTTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg                    <  2:318168/129‑1 (MQ=255)
                                                                                                                              aTTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg                    >  1:318168/1‑129 (MQ=255)
                                                                                                                               ttACTGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTACATACGCTACGAGGTACTTGAt         >  1:936737/1‑139 (MQ=255)
                                                                                                                               ttACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                               <  2:905577/85‑1 (MQ=255)
                                                                                                                               ttACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                               >  1:905577/1‑85 (MQ=255)
                                                                                                                               ttACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGAt         <  1:336040/139‑1 (MQ=255)
                                                                                                                                 aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                               >  2:1548459/1‑51 (MQ=255)
                                                                                                                                 aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                               <  1:1548459/51‑1 (MQ=255)
                                                                                                                                 aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                               >  1:1602129/1‑83 (MQ=255)
                                                                                                                                 aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                               <  2:1602129/83‑1 (MQ=255)
                                                                                                                                 aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTa                                                        <  1:809535/90‑1 (MQ=255)
                                                                                                                                 aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTa                                                        >  2:809535/1‑90 (MQ=255)
                                                                                                                                    gATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACtt    <  1:1266474/139‑1 (MQ=255)
                                                                                                                                    atccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGAt         <  1:890743/130‑1 (MQ=255)
                                                                                                                                     tccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaag                                    >  1:981851/3‑105 (MQ=255)
                                                                                                                                     aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGttt                                       <  2:1302750/103‑1 (MQ=255)
                                                                                                                                     aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGttt                                       >  1:1302750/1‑103 (MQ=255)
                                                                                                                                     aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGAt         >  2:890743/1‑133 (MQ=255)
                                                                                                                                     aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTc   >  1:663577/1‑139 (MQ=255)
                                                                                                                                      tCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCt  >  1:1326263/1‑139 (MQ=255)
                                                                                                                                      tCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGACGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaag                                    <  2:981851/105‑1 (MQ=255)
                                                                                                                                      ccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGa                                                                    <  1:21777/71‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
GTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCT  >  NC_000913/7784‑8055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: