Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,399,985 | (C)7→8 | coding (252/516 nt) | ogt ← | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,399,978 | 1 | . | C | 98.2% | 197.7 / ‑3.9 | 56 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (26/29); total (26/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATA > NC_000913/1399841‑1400101 | gggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑c < 1:53941/139‑1 (MQ=255) ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cc < 1:1066367/139‑1 (MQ=255) ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cc < 1:315453/139‑1 (MQ=255) ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cc < 2:562957/139‑1 (MQ=255) aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cccc < 1:479779/139‑1 (MQ=255) aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cccc < 1:775662/139‑1 (MQ=255) aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cccc < 1:1574112/139‑1 (MQ=255) atacGTTTGCCACACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCGGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg > 1:934502/4‑137 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 1:420363/139‑3 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 1:986539/139‑3 (MQ=255) ccGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccgta < 1:929865/139‑5 (MQ=255) tttGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCa > 2:520141/1‑139 (MQ=255) tttGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCa > 1:1188706/1‑139 (MQ=255) cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTggg < 2:934502/139‑1 (MQ=255) cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTggg < 1:283287/139‑1 (MQ=255) gCACCAACGGTACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGa < 1:726847/139‑1 (MQ=255) aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAg < 2:1021577/139‑1 (MQ=255) cACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaa < 1:94860/139‑1 (MQ=255) gccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATg < 1:520141/139‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGa < 2:724574/109‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGa > 1:724574/1‑109 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaat > 2:437862/1‑120 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaat < 1:437862/120‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATg < 1:1080954/124‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa > 1:1140440/1‑139 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa > 1:213188/1‑139 (MQ=255) cccAATTGCACAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATg > 2:1080954/1‑124 (MQ=255) aTTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAat < 2:1258019/139‑1 (MQ=255) cTCAGCCAGTTGGCCGTAATGCATTACCTTCGCGAAGTGGGTAGTGCGTAGTGTTTTCCAGTATTCGCGCTGAATTCACGTT‑CGCCCTCGTAGAAGTGGGAAGCGCATCAATAATGGTGAGATTTCCGGCAAAATATTc < 1:92454/139‑1 (MQ=255) tCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCa < 2:1188706/139‑1 (MQ=255) aGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCa < 2:136712/92‑1 (MQ=255) aGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCa > 1:136712/1‑92 (MQ=255) tGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaa < 2:581358/101‑1 (MQ=255) tGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaa > 1:581358/1‑101 (MQ=255) tGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTg > 1:39090/1‑139 (MQ=255) ccGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCg > 2:446156/1‑139 (MQ=255) aTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa < 1:446156/139‑1 (MQ=255) aTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa < 1:238652/139‑1 (MQ=255) aTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa < 2:1140440/139‑1 (MQ=255) ttACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGc > 1:50639/1‑139 (MQ=255) ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTggg < 2:587935/74‑1 (MQ=255) ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTggg > 1:587935/1‑74 (MQ=255) tGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGa < 1:811447/139‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑c < 1:84899/52‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑c > 2:84899/1‑52 (MQ=255) cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAg < 1:30790/69‑1 (MQ=255) cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAg > 2:30790/1‑69 (MQ=255) cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa < 2:371115/97‑1 (MQ=255) cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa > 1:371115/1‑97 (MQ=255) gATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg > 2:1155533/1‑49 (MQ=255) gATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 1:1155533/51‑3 (MQ=255) gTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg < 2:1426645/139‑1 (MQ=255) tgagTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCt < 2:947902/117‑1 (MQ=255) tGCGTAGTGTTTTCCAGACTACGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCt > 1:947902/1‑120 (MQ=255) gCGTAGTGTTTTCCAGACTTCGCTCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcg > 2:1210088/1‑139 (MQ=255) cGTAGTGTTTTCCAGACTTCGCTCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcgc < 1:1210088/139‑1 (MQ=255) cGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcgc < 2:1448271/139‑1 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg < 2:122343/135‑1 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg > 1:122343/1‑135 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg > 2:556785/1‑41 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 1:556785/43‑3 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGc < 2:1277896/45‑1 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGc > 1:1277896/1‑45 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGc < 2:722352/45‑1 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGc > 1:722352/1‑45 (MQ=255) gtTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAg > 2:783517/1‑139 (MQ=255) ttttCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaataat > 2:392582/1‑62 (MQ=255) ttttCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaataat < 1:392582/62‑1 (MQ=255) tttCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGcc > 2:4517/1‑139 (MQ=255) tCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCTTAGCAGTGGGAAGCGTATCAATAATACTAAGATTACCTGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCctt < 1:187077/139‑1 (MQ=255) aCTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTg > 2:592074/1‑139 (MQ=255) cgcgCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTAt > 1:1613188/1‑43 (MQ=255) cgcgCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTAt < 2:1613188/43‑1 (MQ=255) gcgcTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATa > 2:1166490/1‑139 (MQ=255) | GGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATA > NC_000913/1399841‑1400101 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |