| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,734,543 | (C)8→9 | coding (492/732 nt) | yfiH ← | UPF0124 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,734,535 | 1 | . | C | 91.9% | 118.6 / 2.9 | 37 | coding (500/732 nt) | yfiH | UPF0124 family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (20/14); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑T‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGC > NC_000913/2734405‑2734662 | aaTTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑T‑‑cccccccc < 1:931029/139‑1 (MQ=255) aaCACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGaa < 2:316662/139‑1 (MQ=255) aCGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:1449872/139‑1 (MQ=255) aCGCTGCCGGGCAAGCAGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCGC‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:1554310/139‑1 (MQ=255) gCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc > 1:1587324/1‑139 (MQ=255) tAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCgaga < 2:809710/139‑1 (MQ=255) tCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 2:489680/1‑114 (MQ=255) tCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 1:489680/114‑1 (MQ=255) aCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaa < 2:1190786/139‑1 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGaa < 1:504711/77‑1 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGaa > 2:504711/1‑77 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc < 1:1525939/100‑1 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc > 2:1525939/1‑100 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAc > 1:980822/1‑139 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAATACAgg > 1:744146/1‑139 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATAAGCAAAACAgg > 1:604977/1‑139 (MQ=255) gaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa > 2:359784/1‑139 (MQ=255) gCTGCACTTGCTTTCGCGTCTACTGCCATAAACGCCTCGCCAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTctct > 2:979605/1‑139 (MQ=255) gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGGGAATATTTTCCGGATTATCAGCAAAGCAGGAAACCGTCTCTTcc > 2:1082079/1‑139 (MQ=255) aCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:501872/1‑76 (MQ=255) aCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:501872/76‑1 (MQ=255) gCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACg > 1:1286900/1‑139 (MQ=255) aGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa > 2:129168/1‑132 (MQ=255) aGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa < 1:129168/132‑1 (MQ=255) gTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 2:1527991/139‑1 (MQ=255) tACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAg > 2:687309/1‑139 (MQ=255) tACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAg > 2:1022199/1‑139 (MQ=255) tACTGCCATAAACGCCTCGCGAACC‑T‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAg < 2:836992/98‑1 (MQ=255) tACTGCCATAAACGCCTCGCGAACC‑T‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAg > 1:836992/1‑98 (MQ=255) aCTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc < 2:152623/65‑1 (MQ=255) aCTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc > 1:152623/1‑65 (MQ=255) aaCGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgccag < 2:980822/139‑1 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:1187140/46‑1 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:1187140/1‑46 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCcagccagc > 1:43531/1‑139 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGACCACCCcagcccgc > 1:408648/1‑139 (MQ=255) cgcgAACC‑TCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTg > 1:988858/1‑44 (MQ=255) cgcgAACC‑TCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTg < 2:988858/44‑1 (MQ=255) | AATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑T‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGC > NC_000913/2734405‑2734662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |