| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,101,039 | (C)7→8 | coding (585/720 nt) | yggN ← | DUF2884 family putative periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,101,032 | 1 | . | C | 93.9% | 160.1 / 2.6 | 49 | coding (592/720 nt) | yggN | DUF2884 family putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base C (21/25); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAG‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCG > NC_000913/3100900‑3101165 | agGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAG‑cccccc > 1:849882/1‑139 (MQ=255) ggATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAG‑ccccccc > 1:1529341/1‑139 (MQ=255) ttattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCCG‑CCCCCCCAgg < 1:372992/139‑1 (MQ=255) tattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAG‑ccccccccagg < 1:810404/139‑5 (MQ=255) ttttAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCt < 1:172972/139‑1 (MQ=255) aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCa < 1:1498601/139‑1 (MQ=255) aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAG‑ccccccccagg > 2:746944/1‑128 (MQ=255) aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAG‑ccccccccagg < 1:746944/132‑5 (MQ=255) tCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGca < 2:871482/139‑1 (MQ=255) tCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGca < 2:1255652/139‑1 (MQ=255) ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAG‑CCCCCCCAGGCTTCCCAGCACAt < 1:111153/139‑1 (MQ=255) tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt > 2:754527/1‑139 (MQ=255) tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt > 1:770558/1‑139 (MQ=255) gttacctCCAAAGTCAAAACGCGGCTTAAAACCTCTTTTCCAAACCTCTTGAAATCTTTTTCCTGCTTTTTCCACTCCGTTTGGATTTGAGGTTGCCGG‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccg < 2:585404/133‑1 (MQ=255) cTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGGTTGCAG‑ccccccccagg > 1:1105822/1‑105 (MQ=255) cTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGGTTGCAG‑ccccccccagg < 2:1105822/109‑5 (MQ=255) cTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccg > 1:1458042/1‑139 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa > 2:283057/1‑139 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa > 1:1543063/1‑139 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAcc > 1:618834/1‑139 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAG‑c < 2:736641/84‑1 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAG‑c > 1:736641/1‑84 (MQ=255) aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGcc < 2:1482723/139‑1 (MQ=255) aaaCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa < 1:1005945/139‑1 (MQ=255) aaCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt < 1:754527/139‑1 (MQ=255) cTTTGCCAAACTGCTGGAAATCTTTTTCCTTCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt < 2:1543063/139‑1 (MQ=255) cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt > 2:777083/1‑139 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGc > 1:1206250/1‑81 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGc < 2:1206250/81‑1 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGt > 2:147846/1‑90 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGt < 1:147846/90‑1 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTaa > 2:569852/1‑139 (MQ=255) ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg < 1:777083/139‑1 (MQ=255) ctgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTc < 2:1458042/139‑1 (MQ=255) ctgctgGAAATCTTTTTCCCGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTc > 1:259908/1‑139 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg > 2:1336799/1‑95 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 1:1336799/95‑1 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTg > 2:205508/1‑139 (MQ=255) ggAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa < 1:569852/139‑1 (MQ=255) cTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc < 1:1550437/139‑1 (MQ=255) ttttCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg > 1:829998/1‑121 (MQ=255) ttttCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg < 2:829998/121‑1 (MQ=255) ttttCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCa > 2:716860/1‑139 (MQ=255) gCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTg > 1:398140/1‑139 (MQ=255) cTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc < 1:190722/139‑1 (MQ=255) cTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc < 1:343223/139‑1 (MQ=255) tttttCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGcc < 1:283057/139‑1 (MQ=255) tCCACTCGGTTTGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGAt < 2:259908/139‑1 (MQ=255) tttGGA‑TTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAAtt > 2:1201961/1‑139 (MQ=255) gAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGAt < 2:1321413/122‑1 (MQ=255) gAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGAt > 1:1321413/1‑122 (MQ=255) aGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt < 2:768716/80‑1 (MQ=255) aGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:768716/1‑80 (MQ=255) tGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc > 2:163806/1‑60 (MQ=255) tGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc < 1:163806/60‑1 (MQ=255) tGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg > 2:355962/1‑85 (MQ=255) tGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg < 1:355962/85‑1 (MQ=255) tGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg < 1:205508/139‑1 (MQ=255) | AGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGA‑TTGAGGATTGCAG‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCG > NC_000913/3100900‑3101165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |