| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,980,957 | (G)6→7 | coding (71/1386 nt) | yifK → | putative APC family amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,980,951 | 1 | . | G | 100.0% | 230.0 / NA | 63 | L22R (CTG→CGG) | yifK | putative APC family amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (32/31); total (32/31) | |||||||||||
CATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAA > NC_000913/3980813‑3981081 | cATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCt < 1:984278/139‑1 (MQ=255) cTCGATGTACCCATAACGATAACCGGTAACACTGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑gggggggcacc > 1:958025/1‑134 (MQ=255) aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGGGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc < 1:102747/139‑1 (MQ=255) aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc < 2:932935/139‑1 (MQ=255) tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGcc < 1:1087535/139‑1 (MQ=255) aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTg > 2:825529/1‑139 (MQ=255) gATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGtt > 2:24005/1‑139 (MQ=255) tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc < 2:52527/132‑1 (MQ=255) tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc > 1:52527/1‑132 (MQ=255) aaCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTAt > 1:111512/1‑139 (MQ=255) aaCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTAt > 1:771879/1‑139 (MQ=255) aCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑ggggg < 1:997095/112‑1 (MQ=255) aCCGGTAACACCGGAAAGCATGCAAACAAAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑ggggg > 2:997095/1‑112 (MQ=255) aaCACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgcc > 1:68014/1‑139 (MQ=255) cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc < 1:825529/139‑1 (MQ=255) acCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTc < 2:572209/118‑1 (MQ=255) acCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTc > 1:572209/1‑118 (MQ=255) cGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTa > 2:1330215/1‑126 (MQ=255) cGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTa < 1:1330215/126‑1 (MQ=255) ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt > 2:520887/1‑139 (MQ=255) ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑ggggg > 2:533522/1‑100 (MQ=255) ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑ggggg < 1:533522/100‑1 (MQ=255) gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa > 1:24826/1‑139 (MQ=255) gCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa > 2:258731/1‑139 (MQ=255) aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg < 2:474250/139‑1 (MQ=255) acacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccggg > 1:1283716/1‑139 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc > 2:901830/1‑99 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc < 1:901830/99‑1 (MQ=255) tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTa > 1:563510/1‑139 (MQ=255) aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 1:24005/139‑1 (MQ=255) aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 2:563510/139‑1 (MQ=255) tGGCAGATCACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCACTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt < 2:958025/139‑1 (MQ=255) tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt < 1:581947/139‑1 (MQ=255) gCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg < 2:68014/139‑1 (MQ=255) gCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg < 2:347319/139‑1 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc > 2:1193833/1‑139 (MQ=255) aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt < 2:1422012/133‑1 (MQ=255) aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt > 1:1422012/1‑133 (MQ=255) cAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat < 2:24826/139‑1 (MQ=255) aCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt > 2:278870/1‑118 (MQ=255) aCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt < 1:278870/118‑1 (MQ=255) aCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatcat > 1:822193/1‑139 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc < 2:104152/69‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc > 1:104152/1‑69 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg < 2:1268501/89‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg > 1:1268501/1‑89 (MQ=255) aGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg < 2:246933/101‑1 (MQ=255) aGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg > 1:246933/1‑101 (MQ=255) cGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 1:215386/80‑1 (MQ=255) cGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 2:215386/1‑80 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg < 1:1233020/68‑1 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg > 2:1233020/1‑68 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑ggggggg > 1:630355/1‑42 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑ggggggg < 2:630355/43‑2 (MQ=255) atCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATggg < 1:1288690/74‑1 (MQ=255) atCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATggg > 2:1288690/1‑74 (MQ=255) aCTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCg < 1:11180/130‑1 (MQ=255) aCTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCg > 2:11180/1‑130 (MQ=255) tCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 1:784131/84‑1 (MQ=255) tCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg > 2:784131/1‑84 (MQ=255) tCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg < 1:1193833/139‑1 (MQ=255) tCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg < 1:520887/139‑1 (MQ=255) aTCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc < 2:1306150/88‑1 (MQ=255) aTCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc > 1:1306150/1‑88 (MQ=255) aTCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat < 2:700297/93‑1 (MQ=255) aTCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat > 1:700297/1‑93 (MQ=255) aTCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGaa > 2:396746/1‑139 (MQ=255) aTCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGaa > 2:1244410/1‑139 (MQ=255) aTCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGaa > 1:1338736/1‑139 (MQ=255) cGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt > 2:1023936/1‑81 (MQ=255) cGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt < 1:1023936/81‑1 (MQ=255) | CATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAA > NC_000913/3980813‑3981081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |