Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,246,411	(T)5→6	coding (9/1191 nt)	malE ←	maltose transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,246,406	1	.	T	100.0%	140.3 / NA	44	T5K (ACA→AAA)	malE	maltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (21/23);  total (21/23)

CTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTG‑TTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCCACCGTCGCTTTGTGTG  >  NC_000913/4246275‑4246536                                                                                                                                     |                                                                                                                                   cTTCATCGAGACCGTTATAGCCTTTATCGCCCTTAATCCAGATTACCAGTTTCCCCTCTTCGATTTTAGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGCATAATGCGAGGATGCGTGCACCTG‑tttttta                                                                                                                             <  1:853983/139‑3 (MQ=255) cTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTG‑tttttta                                                                                                                             <  1:1160476/139‑3 (MQ=255)   tCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTG‑ttttttatt                                                                                                                           <  2:3399/139‑5 (MQ=255)        gagaCCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCAt                                                                                                                       <  1:724207/139‑1 (MQ=255)                       tttATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCttg                                                                                                        <  2:732755/139‑1 (MQ=255)                         tATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCttgtt                                                                                                      >  2:871652/1‑139 (MQ=255)                               cGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGaa                                                                                                >  2:96390/1‑139 (MQ=255)                                 ttAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCa                                                                                                                        <  2:505868/113‑1 (MQ=255)                                 ttAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCa                                                                                                                        >  1:505868/1‑113 (MQ=255)                                          aTTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGaa                                                                                     <  2:164416/139‑1 (MQ=255)                                                   ttACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTG‑tttttta                                                                                                                             >  2:943074/1‑87 (MQ=255)                                                   ttACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTG‑tttttta                                                                                                                             <  1:943074/89‑3 (MQ=255)                                                    tACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGt                                                                                                             <  1:84689/105‑1 (MQ=255)                                                    tACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGt                                                                                                             >  2:84689/1‑105 (MQ=255)                                                    tACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTaaa                                                                           <  2:256526/139‑1 (MQ=255)                                                    tACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTaaa                                                                           <  2:289155/139‑1 (MQ=255)                                                    tACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTaaa                                                                           >  2:1266108/1‑139 (MQ=255)                                                           ttcGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTc                                                                                                                         <  2:196193/86‑1 (MQ=255)                                                           ttcGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTc                                                                                                                         >  1:196193/1‑86 (MQ=255)                                                            tcGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGActct                                                                   >  1:53658/1‑139 (MQ=255)                                                            tcGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTG‑tttttta                                                                                                                             <  1:1557694/80‑3 (MQ=255)                                                            tcGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTG‑tttttta                                                                                                                             >  2:1557694/1‑78 (MQ=255)                                                              gATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAg                                                                 <  2:397879/139‑1 (MQ=255)                                                                   tGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTG‑tttttta                                                                                                                             <  2:1180712/73‑3 (MQ=255)                                                                   tGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTG‑tttttta                                                                                                                             >  1:1180712/1‑71 (MQ=255)                                                                      cGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTta                                                         <  1:871652/139‑1 (MQ=255)                                                                          agCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTtat                                                        >  2:910908/1‑136 (MQ=255)                                                                          agCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTtat                                                        <  1:910908/136‑1 (MQ=255)                                                                               aGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAAc                                                <  1:1266108/139‑1 (MQ=255)                                                                                   ggAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACctct                                            <  2:53658/139‑1 (MQ=255)                                                                                           tcatcGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAg                                                                 <  2:1193995/110‑1 (MQ=255)                                                                                           tcatcGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAg                                                                 >  1:1193995/1‑110 (MQ=255)                                                                                           tcatcGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACctct                                            <  1:171334/131‑1 (MQ=255)                                                                                           tcatcGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACctct                                            >  2:171334/1‑131 (MQ=255)                                                                                                         gCGGATAATGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACg                      >  1:1505377/1‑139 (MQ=255)                                                                                                                 tGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGttt                                                              <  2:97902/91‑1 (MQ=255)                                                                                                                 tGCGAGGATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGttt                                                              >  1:97902/1‑91 (MQ=255)                                                                                                                      ggATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTaaa                                                                           >  2:318678/1‑73 (MQ=255)                                                                                                                      ggATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTaaa                                                                           <  1:318678/73‑1 (MQ=255)                                                                                                                       gATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACgg                                                    <  2:588213/95‑1 (MQ=255)                                                                                                                       gATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACgg                                                    >  1:588213/1‑95 (MQ=255)                                                                                                                       gATGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCCACCGTCGCtt        <  1:96390/139‑1 (MQ=255)                                                                                                                         tGCGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCCACCGTCGCTTtg      >  1:1314518/1‑139 (MQ=255)                                                                                                                           cGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGaa                                                                                                >  1:875393/1‑47 (MQ=255)                                                                                                                           cGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGaa                                                                                                <  2:875393/47‑1 (MQ=255)                                                                                                                           cGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAg                                                                 <  1:1260888/78‑1 (MQ=255)                                                                                                                           cGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAg                                                                 >  2:1260888/1‑78 (MQ=255)                                                                                                                           cGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCCAcc               >  1:593431/1‑128 (MQ=255)                                                                                                                           cGTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCCAcc               <  2:593431/128‑1 (MQ=255)                                                                                                                            gTGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCCACCGTCGCTTtgtgt   >  1:33486/1‑139 (MQ=255)                                                                                                                             tGCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCCACCGTCGCTTtgtgtg  >  1:580040/1‑139 (MQ=255)                                                                                                                              gCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACAcc                                                                               >  2:653536/1‑61 (MQ=255)                                                                                                                              gCACCTGTTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACAcc                                                                               <  1:653536/61‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   CTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTG‑TTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCCACCGTCGCTTTGTGTG  >  NC_000913/4246275‑4246536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑