Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,474,138 (A)6→7 coding (15/594 nt) bdcR → transcriptional repressor for divergent bdcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,474,1321.A96.0% 236.9 / 1.6 75coding (9/594 nt)bdcRtranscriptional repressor for divergent bdcA
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base A (42/30);  total (44/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

CCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGT  >  NC_000913/4473998‑4474264
                                                                                                                                       |                                                                                                                                     
ccGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTaaaa                                                                                                                                   >  2:1283167/1‑139 (MQ=255)
ccGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTaaaa                                                                                                                                   >  1:639844/1‑139 (MQ=255)
     aCCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTaaaaaaaca                                                                                                                              <  2:639844/139‑4 (MQ=255)
               ccACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGtt                                                                                                                    >  1:644223/1‑139 (MQ=255)
                               cTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGAc                                                                                                    >  2:740767/1‑139 (MQ=255)
                                  tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTc                                                                                                                   <  2:1273960/121‑1 (MQ=255)
                                  tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTc                                                                                                                   >  1:1273960/1‑121 (MQ=255)
                                  tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGtt                                                                                                 >  1:1585872/1‑139 (MQ=255)
                                  tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGtt                                                                                                 >  2:1594011/1‑139 (MQ=255)
                                       ccTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTGTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAATCAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCt                                                                                            >  2:1010126/1‑139 (MQ=255)
                                           tAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTg                                                                                        >  1:526381/1‑139 (MQ=255)
                                            aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGa                                                                                       >  1:1575600/1‑139 (MQ=255)
                                            aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGa                                                                                       >  2:862619/1‑139 (MQ=255)
                                             aaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  1:1010126/139‑1 (MQ=255)
                                             aaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:65355/139‑1 (MQ=255)
                                                   cccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgt                                                                                                              <  2:35476/109‑1 (MQ=255)
                                                   cccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgt                                                                                                              >  1:35476/1‑109 (MQ=255)
                                                     cATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                              >  1:1544317/1‑139 (MQ=255)
                                                              tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc                                                                     >  1:1016914/1‑139 (MQ=255)
                                                              tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc                                                                     >  1:1112937/1‑139 (MQ=255)
                                                                cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                   >  2:808957/1‑139 (MQ=255)
                                                                cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTa                                                                                                                                      <  2:1260874/72‑1 (MQ=255)
                                                                cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTa                                                                                                                                      >  1:1260874/1‑72 (MQ=255)
                                                                     tttATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTg                                                              >  2:1250262/1‑139 (MQ=255)
                                                                        attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtt                                                           >  1:1044937/1‑139 (MQ=255)
                                                                          tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtttt                                                         >  1:1299034/1‑139 (MQ=255)
                                                                           atGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                        <  2:316467/139‑1 (MQ=255)
                                                                              tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                     >  2:1348511/1‑139 (MQ=255)
                                                                              tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                     >  1:406097/1‑139 (MQ=255)
                                                                              tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                     >  2:101792/1‑139 (MQ=255)
                                                                               aaCATGCATTATAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAcc                                                    <  2:1044937/139‑1 (MQ=255)
                                                                                  aTGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa                                                 <  1:1283167/139‑1 (MQ=255)
                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCCCTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGa                                                                                                     <  2:337418/85‑1 (MQ=255)
                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                        >  2:1486349/1‑67 (MQ=255)
                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                        <  1:1486349/67‑1 (MQ=255)
                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                   >  2:1379064/1‑120 (MQ=255)
                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                   <  1:1379064/120‑1 (MQ=255)
                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  1:585277/1‑139 (MQ=255)
                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  2:777423/1‑139 (MQ=255)
                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  2:1033614/1‑139 (MQ=255)
                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGa                                                                                                     >  1:337418/1‑85 (MQ=255)
                                                                                   tGCATTACAAAACTGTTTTAACTTTCTATCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  1:225588/1‑139 (MQ=255)
                                                                                      aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg                                             >  1:525754/1‑139 (MQ=255)
                                                                                         aCAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGttt                                          >  1:463076/1‑139 (MQ=255)
                                                                                          cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTc                                         >  2:1229492/1‑139 (MQ=255)
                                                                                          cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTc                                         <  2:198053/139‑1 (MQ=255)
                                                                                           aaaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAg                                                                                                                >  2:1186945/1‑67 (MQ=255)
                                                                                           aaaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAg                                                                                                                <  1:1186945/67‑1 (MQ=255)
                                                                                             aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAAtctc                                                                                                                         >  2:363074/1‑56 (MQ=255)
                                                                                             aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAAtctc                                                                                                                         <  1:363074/56‑1 (MQ=255)
                                                                                                 gTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaa                                                  <  1:424026/123‑1 (MQ=255)
                                                                                                 gTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaa                                                  >  2:424026/1‑123 (MQ=255)
                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                        >  2:1242604/1‑116 (MQ=255)
                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                        <  1:1242604/116‑1 (MQ=255)
                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCt                                   <  2:43941/137‑1 (MQ=255)
                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCt                                   >  1:43941/1‑137 (MQ=255)
                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 <  1:1574625/139‑1 (MQ=255)
                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  2:1289109/1‑139 (MQ=255)
                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  2:1040118/1‑139 (MQ=255)
                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  1:1504545/1‑139 (MQ=255)
                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  1:474171/1‑139 (MQ=255)
                                                                                                     tAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg                              <  1:1289109/139‑1 (MQ=255)
                                                                                                           tCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGc                        <  1:1250262/139‑1 (MQ=255)
                                                                                                            cTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt                       >  1:120562/1‑139 (MQ=255)
                                                                                                             tatCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTg                      <  2:225588/137‑1 (MQ=255)
                                                                                                             tGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTg                      <  2:1112937/139‑1 (MQ=255)
                                                                                                                 aaCAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt                  <  2:525754/139‑1 (MQ=255)
                                                                                                                      gTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa                                                 >  2:344500/1‑103 (MQ=255)
                                                                                                                      gTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa                                                 <  1:344500/103‑1 (MQ=255)
                                                                                                                       ttttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTTTGCTGAAGTTACTGAt           <  2:735164/139‑1 (MQ=255)
                                                                                                                        tttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGATCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt           <  1:1033614/139‑1 (MQ=255)
                                                                                                                        tttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt           <  2:463076/139‑1 (MQ=255)
                                                                                                                        tttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt           <  2:585277/139‑1 (MQ=255)
                                                                                                                            tAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc       <  1:101792/139‑1 (MQ=255)
                                                                                                                             aaTGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCt      <  2:644223/139‑1 (MQ=255)
                                                                                                                              aTGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGTTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCtt     <  1:740767/139‑1 (MQ=255)
                                                                                                                                ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg   >  2:1423322/1‑139 (MQ=255)
                                                                                                                                 gTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  1:941514/1‑93 (MQ=255)
                                                                                                                                 gTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                <  2:941514/93‑1 (MQ=255)
                                                                                                                                 gTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGt  >  1:1182081/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGT  >  NC_000913/4473998‑4474264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: