Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	491,776	T→C	V122A (GTT→GCT)	apt →	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	491,776	0	T	C	100.0%	215.4 / NA	60	V122A (GTT→GCT)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/27);  total (33/27)

TGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGC  >  NC_000913/491644‑491897                                                                                                                                     |                                                                                                                          tgggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtggt                                                                                                                     <  1:737531/139‑1 (MQ=255) tgggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtgg                                                                                                                      >  2:161059/1‑138 (MQ=255) tgggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtgg                                                                                                                      <  1:161059/138‑1 (MQ=255)             ttGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACct                                                                                                         >  1:769205/1‑139 (MQ=255)                   cGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGc                                                                                                   <  2:1131228/139‑1 (MQ=255)                     gTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGTCCTGCTGGCaa                                                                                                 >  2:1044536/1‑139 (MQ=255)                     gTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCaa                                                                                                 <  2:1055541/139‑1 (MQ=255)                         gTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcgg                                                                                             <  2:1393363/139‑1 (MQ=255)                          tAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggc                                                                                            >  2:1343470/1‑139 (MQ=255)                              ccGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCa                                                                                        <  1:275345/139‑1 (MQ=255)                                gggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACt                                                                                      >  1:1028112/1‑139 (MQ=255)                                 ggCAAACTGCCGCGTGAAAGCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTGGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTa                                                                                     >  2:1075445/1‑139 (MQ=255)                                  gCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGTGATCCACGTTGATGCCATCAACCCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTAt                                                                                    >  1:1244038/1‑139 (MQ=255)                                             cgTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCg                                                                         <  2:769205/139‑1 (MQ=255)                                                         aGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATcc                                                             <  1:1343470/139‑1 (MQ=255)                                                              aaCTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgtcgt                                                        >  1:40763/1‑139 (MQ=255)                                                               aCTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCgtcgtc                                                       >  1:258818/1‑139 (MQ=255)                                                                cTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc                                                                              >  2:223583/1‑115 (MQ=255)                                                                cTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc                                                                              <  1:223583/115‑1 (MQ=255)                                                                   aCGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTggg                                                   >  1:206581/1‑139 (MQ=255)                                                                     gACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGggtg                                                 >  2:892301/1‑139 (MQ=255)                                                                          ggAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgtcgt                                                        <  1:62680/127‑1 (MQ=255)                                                                          ggAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgtcgt                                                        >  2:62680/1‑127 (MQ=255)                                                                          ggAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGaa                                            <  2:40763/139‑1 (MQ=255)                                                                          ggAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGaa                                            <  1:892301/139‑1 (MQ=255)                                                                                     ccGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtggt                                                                                                                     <  2:436268/55‑1 (MQ=255)                                                                                     ccGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtggt                                                                                                                     >  1:436268/1‑55 (MQ=255)                                                                                     ccGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                                          <  2:272687/82‑1 (MQ=255)                                                                                     ccGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                                          >  1:272687/1‑82 (MQ=255)                                                                                     ccGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCt                                                      <  2:699409/118‑1 (MQ=255)                                                                                     ccGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCt                                                      >  1:699409/1‑118 (MQ=255)                                                                                        aTCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATcc                                                             >  1:860490/1‑108 (MQ=255)                                                                                        aTCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATcc                                                             <  2:860490/108‑1 (MQ=255)                                                                                             cTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGAc                                                                           >  1:266611/1‑89 (MQ=255)                                                                                             cTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGAc                                                                           <  2:266611/89‑1 (MQ=255)                                                                                                gagaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTa                      >  2:168850/1‑139 (MQ=255)                                                                                                  gaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATc                    >  2:1399239/1‑139 (MQ=255)                                                                                                    tCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGTCCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                           >  2:633404/1‑130 (MQ=255)                                                                                                    tCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGTCCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                           <  1:633404/130‑1 (MQ=255)                                                                                                     ccACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGga                                                                                                               >  2:1308474/1‑45 (MQ=255)                                                                                                     ccACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGga                                                                                                               <  1:1308474/45‑1 (MQ=255)                                                                                                     ccACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAAc                 <  2:1404785/139‑1 (MQ=255)                                                                                                     ccACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAAc                 >  1:268118/1‑139 (MQ=255)                                                                                                      cACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAAcc                <  1:168850/139‑1 (MQ=255)                                                                                                       aCGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCt               >  1:942679/1‑139 (MQ=255)                                                                                                          ttGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGtt            >  2:1186236/1‑139 (MQ=255)                                                                                                           tGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                           <  1:113524/123‑1 (MQ=255)                                                                                                           tGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                           >  2:113524/1‑123 (MQ=255)                                                                                                           tGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTc           >  2:1340154/1‑139 (MQ=255)                                                                                                           tGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTc           >  2:56435/1‑139 (MQ=255)                                                                                                               gCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                           <  2:1487406/119‑1 (MQ=255)                                                                                                               gCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                           >  1:1487406/1‑119 (MQ=255)                                                                                                                   tCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTcgg   >  1:543684/1‑139 (MQ=255)                                                                                                                   tCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATT‑TCAACCTGTTCGATCTcggc  <  1:845544/139‑1 (MQ=255)                                                                                                                     aaaCCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGgtggtg                                              >  2:550865/1‑94 (MQ=255)                                                                                                                     aaaCCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGgtggtg                                              <  1:550865/94‑1 (MQ=255)                                                                                                                           ggCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCa                                                                                                  <  1:947803/36‑1 (MQ=255)                                                                                                                           ggCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCa                                                                                                  >  2:947803/1‑36 (MQ=38)                                                                                                                             cGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGAc                                                                           >  1:941036/1‑57 (MQ=255)                                                                                                                             cGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGAc                                                                           <  2:941036/57‑1 (MQ=255)                                                                                                                                     |                                                                                                                          TGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGC  >  NC_000913/491644‑491897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑