Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	522,143	A→G	E576E (GAA→GAG)	ybbP →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	522,143	0	A	G	100.0%	258.4 / NA	72	E576E (GAA→GAG)	ybbP	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/36);  total (36/36)

AGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTT  >  NC_000913/522013‑522271                                                                                                                                   |                                                                                                                                 aGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCt                                                                                                                          >  2:464201/1‑139 (MQ=255)        aGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGaaa                                                                                                                   >  2:1350452/1‑139 (MQ=255)            ggTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCt                                                                                                               >  1:1226148/1‑139 (MQ=255)                    aGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGtt                                                                                                       >  2:488781/1‑139 (MQ=255)                    aGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTg                                                                                                      >  1:473504/1‑139 (MQ=255)                     gATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTg                                                                                                      <  1:1403389/139‑1 (MQ=255)                      aTGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGc                                                                                                     >  2:12662/1‑139 (MQ=255)                      aTGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGc                                                                                                     >  2:1165961/1‑139 (MQ=255)                       tGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    <  1:1165961/139‑1 (MQ=255)                       tGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    <  1:8973/139‑1 (MQ=255)                       tGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    <  1:1513897/139‑1 (MQ=255)                        gAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGccc                                                                                                   >  1:837100/1‑139 (MQ=255)                        gAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGccc                                                                                                   >  1:688978/1‑139 (MQ=255)                        gAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGccc                                                                                                   >  1:585859/1‑139 (MQ=255)                         aGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCt                                                                                                  <  1:464201/139‑1 (MQ=255)                          ggCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTc                                                                                                 >  2:1527086/1‑139 (MQ=255)                                   ccGCGAACTCAATCTTACCTGGC‑AAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCg                                                                                       <  2:473504/139‑1 (MQ=255)                                      cgAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTg                                                                                     <  2:211700/139‑1 (MQ=255)                                                 ttACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATggg                                                                          <  1:12662/139‑1 (MQ=255)                                                   aCCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCg                                                                        <  2:688978/139‑1 (MQ=255)                                                    ccTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    <  1:492487/110‑1 (MQ=255)                                                    ccTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    >  2:492487/1‑110 (MQ=255)                                                       ggCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATAc                                                                    >  1:856421/1‑139 (MQ=255)                                                         cAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTg                                                                                                      >  1:476592/1‑103 (MQ=255)                                                         cAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTg                                                                                                      <  2:476592/103‑1 (MQ=255)                                                         cAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATAccc                                                                  >  2:290648/1‑139 (MQ=255)                                                           aaaTACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAg                                                                >  2:1509083/1‑139 (MQ=255)                                                                  cgGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTcc                                                         >  2:57161/1‑139 (MQ=255)                                                                         aTCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTaa                                                  >  1:51951/1‑139 (MQ=255)                                                                         aTCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTaa                                                  <  2:1438613/139‑1 (MQ=255)                                                                                   gATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGc                                        <  1:1350452/139‑1 (MQ=255)                                                                                   gATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGc                                        >  2:1275769/1‑139 (MQ=255)                                                                                    aTTGTCGCTGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGcc                                       >  1:375735/1‑139 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGTAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGcc                                       >  1:107087/1‑139 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGa                                                                                                                     >  2:1176090/1‑61 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGa                                                                                                                     <  1:1176090/61‑1 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTg                                                                                                      >  2:1490230/1‑76 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTg                                                                                                      <  1:1490230/76‑1 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCt                                                                                                  >  1:497390/1‑80 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCt                                                                                                  <  2:497390/80‑1 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATggg                                                                          <  2:936774/104‑1 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATggg                                                                          >  1:936774/1‑104 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCcgcg                                                      >  1:342589/1‑124 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCcgcg                                                      <  2:342589/124‑1 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGAc                                            >  1:1216756/1‑134 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGAc                                            <  2:1216756/134‑1 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAg                                         <  1:278776/137‑1 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAg                                         >  2:278776/1‑137 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGcc                                       <  1:1509083/139‑1 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGcc                                       >  2:1302197/1‑139 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCCGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTaa                                                  >  1:695965/1‑128 (MQ=255)                                                                                    aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCCGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTaa                                                  <  2:695965/128‑1 (MQ=255)                                                                                     ttGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCt                                      >  2:638699/1‑139 (MQ=255)                                                                                     ttGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTCGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCt                                      <  2:856421/139‑1 (MQ=255)                                                                                       gTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTgc                                    <  2:837100/139‑1 (MQ=255)                                                                                        tCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCt                                                                                                  >  1:656514/1‑76 (MQ=255)                                                                                        tCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCt                                                                                                  <  2:656514/76‑1 (MQ=255)                                                                                        tCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTgcg                                   >  2:1579938/1‑139 (MQ=255)                                                                                        tCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTgcg                                   <  1:1527086/139‑1 (MQ=255)                                                                                          gCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCcgc                                                       <  2:1340429/117‑1 (MQ=255)                                                                                          gCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCcgc                                                       >  1:1340429/1‑117 (MQ=255)                                                                                               tAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTg                            <  1:1340986/139‑1 (MQ=255)                                                                                                 aCTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGa                          <  1:488781/139‑1 (MQ=255)                                                                                                       cgccAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGa                    <  2:375735/139‑1 (MQ=255)                                                                                                              aGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTg             <  1:290648/139‑1 (MQ=255)                                                                                                                  gATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGc         <  1:1579938/139‑1 (MQ=255)                                                                                                                  gATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGc         <  1:866464/139‑1 (MQ=255)                                                                                                                    tGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGa                          <  2:577409/120‑1 (MQ=255)                                                                                                                    tGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGa                          >  1:577409/1‑120 (MQ=255)                                                                                                                         tgtCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAAttt  <  2:107087/139‑1 (MQ=255)                                                                                                                          gtCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATggg                                                                          <  2:518174/66‑1 (MQ=255)                                                                                                                          gtCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATggg                                                                          >  1:518174/1‑66 (MQ=255)                                                                                                                                   |                                                                                                                                 AGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTT  >  NC_000913/522013‑522271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑