Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	607,988	G→A	*51* (TAG→TAA)	hokE →	toxic polypeptide, small

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	607,988	0	G	A	100.0%	239.3 / NA	74	*51* (TAG→TAA)	hokE	toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/39);  total (35/39)

TGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA  >  NC_000913/607856‑608107                                                                                                                                     |                                                                                                                        tGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTtg                                                                                                                   >  2:926220/1‑139 (MQ=255) tGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTtg                                                                                                                   >  1:1380964/1‑139 (MQ=255)   cGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTtgtg                                                                                                                 >  1:1148356/1‑139 (MQ=255)     gCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                               <  1:1201236/139‑1 (MQ=255)     gCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                               <  2:1380964/139‑1 (MQ=255)                   gtgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCAt                                                                                                 >  2:284171/1‑139 (MQ=255)                    tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATa                                                                                                >  1:1373116/1‑139 (MQ=255)                         aaCGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAgcgc                                                                                           >  1:668640/1‑139 (MQ=255)                         aaCGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAgcgc                                                                                           <  2:1039778/139‑1 (MQ=255)                          aCGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                          <  1:308058/139‑1 (MQ=255)                          aCGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                          <  1:284171/139‑1 (MQ=255)                              tGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAgcg                                                                                            >  1:1269211/1‑133 (MQ=255)                              tGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAgcg                                                                                            <  2:1269211/133‑1 (MQ=255)                              tGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACt                                                                                      >  2:623234/1‑139 (MQ=255)                              tGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACt                                                                                      >  2:1134898/1‑139 (MQ=255)                                cTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAgcgc                                                                                           >  1:663639/1‑132 (MQ=255)                                cTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAgcgc                                                                                           <  2:663639/132‑1 (MQ=255)                                 tGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTaa                                                                                   <  1:1375859/139‑1 (MQ=255)                                    gATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                <  1:1134898/139‑1 (MQ=255)                                    gATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                <  2:668640/139‑1 (MQ=255)                                    gATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTCAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                <  1:1046087/139‑1 (MQ=255)                                          cGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTgg                                                                          >  1:831723/1‑139 (MQ=255)                                               cTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTa                                                                     <  1:926220/139‑1 (MQ=255)                                                 ggTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTCTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACg                                                                   <  2:1022233/139‑1 (MQ=255)                                                  gTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGc                                                                  >  1:1011878/1‑139 (MQ=255)                                                      gagaCTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                                                              <  2:831723/139‑1 (MQ=255)                                                                 tgtgAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAc                                                   >  1:786626/1‑139 (MQ=255)                                                                     aGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGtt                                                                                                                    >  1:416123/1‑70 (MQ=255)                                                                     aGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGtt                                                                                                                    <  2:416123/70‑1 (MQ=255)                                                                         tACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGAt                                           >  1:166965/1‑139 (MQ=255)                                                                           cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCggg                                                                                                             >  1:626523/1‑71 (MQ=255)                                                                           cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCggg                                                                                                             <  2:626523/71‑1 (MQ=255)                                                                               gAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                               >  2:1461131/1‑65 (MQ=255)                                                                               gAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                               <  1:1461131/65‑1 (MQ=255)                                                                               gAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGa                                                                                                           >  2:257066/1‑69 (MQ=255)                                                                               gAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGa                                                                                                           <  1:257066/69‑1 (MQ=255)                                                                                    aaCGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACtctc                                <  2:873290/139‑1 (MQ=255)                                                                                        tAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCg                                                                                                                      >  2:444933/1‑49 (MQ=255)                                                                                        tAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCg                                                                                                                      <  1:444933/49‑1 (MQ=255)                                                                                        tAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                   >  2:458682/1‑132 (MQ=255)                                                                                        tAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                   <  1:458682/132‑1 (MQ=255)                                                                                          atatTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATa                          >  2:685670/1‑139 (MQ=255)                                                                                                aGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCCTAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGCTTTAACGTGCCGCTGCATTACTCTCACGATAACTgca                    >  1:857623/1‑137 (MQ=21)                                                                                                aGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                    >  1:1435436/1‑75 (MQ=255)                                                                                                aGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                    <  2:1435436/75‑1 (MQ=255)                                                                                                  tttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGt                                                                                                                     >  2:810498/1‑40 (MQ=255)                                                                                                  tttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGt                                                                                                                     <  1:810498/40‑1 (MQ=255)                                                                                                  tttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                          <  2:333316/67‑1 (MQ=255)                                                                                                  tttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                          >  1:333316/1‑67 (MQ=255)                                                                                                     aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                          >  1:632262/1‑64 (MQ=255)                                                                                                     aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                          <  2:632262/64‑1 (MQ=255)                                                                                                     aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                   >  1:1205148/1‑119 (MQ=255)                                                                                                     aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                   <  2:1205148/119‑1 (MQ=255)                                                                                                       ggCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGt                                                                                                         <  2:1514542/47‑1 (MQ=255)                                                                                                       ggCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGt                                                                                                         >  1:1514542/1‑47 (MQ=255)                                                                                                            ttttcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGTTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTgg        <  2:857623/136‑1 (MQ=255)                                                                                                            ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCggg                                                                                                             <  1:513925/38‑1 (MQ=255)                                                                                                            ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCggg                                                                                                             >  2:513925/1‑38 (MQ=255)                                                                                                            ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                <  1:410102/67‑1 (MQ=255)                                                                                                            ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                >  2:410102/1‑67 (MQ=255)                                                                                                             tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc       <  2:786626/139‑1 (MQ=255)                                                                                                             tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc       <  2:1373116/139‑1 (MQ=255)                                                                                                             tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc       <  2:166965/139‑1 (MQ=255)                                                                                                             tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGAGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc       <  2:1011878/139‑1 (MQ=255)                                                                                                                  cTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGCTTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCata  >  2:855838/1‑139 (MQ=255)                                                                                                                  cTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCata  <  1:623234/139‑1 (MQ=255)                                                                                                                      cGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                                                       >  2:371989/1‑66 (MQ=255)                                                                                                                      cGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                                                       <  1:371989/66‑1 (MQ=255)                                                                                                                           cGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtgt                                                                            >  1:83950/1‑56 (MQ=255)                                                                                                                           cGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtgt                                                                            <  2:83950/56‑1 (MQ=255)                                                                                                                             aagaagTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                          <  2:1126307/40‑1 (MQ=39)                                                                                                                             aagaagTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                          >  1:1126307/1‑40 (MQ=255)                                                                                                                                 agTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                   >  1:307694/1‑91 (MQ=255)                                                                                                                                 agTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                   <  2:307694/91‑1 (MQ=255)                                                                                                                                     |                                                                                                                        TGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA  >  NC_000913/607856‑608107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑