Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	677,889	G→A	A159T (GCC→ACC)	djlB →	putative HscC co‑chaperone, uncharacterized J domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	677,889	0	G	A	100.0%	147.5 / NA	46	A159T (GCC→ACC)	djlB	putative HscC co‑chaperone, uncharacterized J domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)

TCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACA  >  NC_000913/677754‑678017                                                                                                                                        |                                                                                                                                 tCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCa                                                                                                                               <  2:1483092/139‑1 (MQ=255)     tgtgCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGc                                                                                                                           >  2:176936/1‑139 (MQ=255)                 ccATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGG‑AATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGtt                                                                                                              >  1:1486447/1‑139 (MQ=255)                   aTGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttt                                                                                                             >  1:1092287/1‑139 (MQ=255)                     gAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttttt                                                                                                           <  2:893754/139‑1 (MQ=255)                     gAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttttt                                                                                                           <  2:31065/139‑1 (MQ=255)                     gAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttttt                                                                                                           <  2:1480056/139‑1 (MQ=255)                        cacTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                   <  2:477380/128‑1 (MQ=255)                        cacTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                   >  1:477380/1‑128 (MQ=255)                                aTTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCGTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGAc                                                                                                <  1:1141141/139‑1 (MQ=255)                                aTTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGAc                                                                                                >  1:247527/1‑139 (MQ=255)                                     tCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTc                                                                                           >  2:1284095/1‑139 (MQ=255)                                         tCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTCGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCa                                                                                       >  1:974160/1‑139 (MQ=255)                                                cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttttt                                                                                                           <  2:270777/112‑1 (MQ=255)                                                cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttttt                                                                                                           >  1:270777/1‑112 (MQ=255)                                                            aGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATACTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCaa                                                                    >  1:1097916/1‑139 (MQ=255)                                                               aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCAt                                                                                      <  2:360705/118‑1 (MQ=255)                                                               aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCAt                                                                                      >  1:360705/1‑118 (MQ=255)                                                               aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTATATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAAt                                                                 >  1:941141/1‑139 (MQ=255)                                                                     aTTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACAcc                                                           >  1:1534280/1‑139 (MQ=255)                                                                       tGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCAt                                                         <  2:941141/139‑1 (MQ=255)                                                                            tggtTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAtt                                                    <  1:176936/139‑1 (MQ=255)                                                                                        gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGc                                                                                                                           >  1:1398876/1‑56 (MQ=255)                                                                                        gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGc                                                                                                                           <  2:1398876/56‑1 (MQ=255)                                                                                        gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAggg                                                                                                                     >  2:38337/1‑62 (MQ=255)                                                                                        gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAggg                                                                                                                     <  1:38337/62‑1 (MQ=255)                                                                                        gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGt                                        >  2:402743/1‑139 (MQ=255)                                                                                        gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGt                                        >  1:1214865/1‑139 (MQ=255)                                                                                        gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGt                                        >  1:90676/1‑139 (MQ=255)                                                                                        gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGt                                        >  1:872698/1‑139 (MQ=255)                                                                                             tACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTg                                                                       >  1:268753/1‑103 (MQ=255)                                                                                             tACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTg                                                                       <  2:268753/103‑1 (MQ=255)                                                                                             tACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCt                                   >  1:1353545/1‑139 (MQ=255)                                                                                               ccGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCg                                 >  2:949913/1‑139 (MQ=255)                                                                                                        tAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTc                        <  2:90676/139‑1 (MQ=255)                                                                                                          gACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCAt                      <  2:1092287/139‑1 (MQ=255)                                                                                                           aCGAAGAAGTACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATc                     <  1:949913/139‑1 (MQ=255)                                                                                                           aCGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATc                     <  2:1486447/139‑1 (MQ=255)                                                                                                              aagaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCa                  <  1:1284095/139‑1 (MQ=255)                                                                                                               agaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCAtt                                                                                            >  1:449667/1‑64 (MQ=255)                                                                                                               agaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCAtt                                                                                            <  2:449667/64‑1 (MQ=255)                                                                                                                  agaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCca              <  2:1534280/139‑1 (MQ=255)                                                                                                                  agaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCca              <  2:1214865/139‑1 (MQ=255)                                                                                                                    aaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCcaca            <  2:247527/139‑1 (MQ=255)                                                                                                                         gAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGc       <  1:402743/139‑1 (MQ=255)                                                                                                                              cTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACa  >  1:1366321/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                 TCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACA  >  NC_000913/677754‑678017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑