Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	746,723	C→T	H263H (CAC→CAT)  *349* (TAG→TAA)	nei → abrB ←	endonuclease VIII and 5‑formyluracil/5‑hydroxymethyluracil DNA glycosylase regulator of aidB expression, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	746,723	0	C	T	100.0%	218.8 / NA	67	H263H (CAC→CAT)  *349* (TAG→TAA)	nei abrB	endonuclease VIII and 5‑formyluracil/5‑hydroxymethyluracil DNA glycosylase regulator of aidB expression, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/34);  total (33/34)

AGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCT  >  NC_000913/746593‑746842                                                                                                                                   |                                                                                                                        aGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGa                                                                                                                 <  2:727848/139‑1 (MQ=255)      catGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCt                                                                                                            >  2:1485100/1‑139 (MQ=255)       atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCtt                                                                                                           >  2:306599/1‑139 (MQ=255)           ggCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGgc                                                                                                       >  1:64037/1‑139 (MQ=255)           ggCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGgc                                                                                                       >  1:724/1‑139 (MQ=255)              gcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                    <  1:1485100/139‑1 (MQ=255)              gcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                    <  1:1553974/139‑1 (MQ=255)                  tttCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                >  1:1316539/1‑139 (MQ=255)                  tttCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                <  2:1316539/139‑1 (MQ=255)                     cGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTACCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTg                                                                                             >  1:213291/1‑139 (MQ=255)                          tAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                <  2:791663/131‑1 (MQ=255)                          tAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                >  1:791663/1‑131 (MQ=255)                            aGGTTTTTCATCGAGATGGCGAACCGTGTGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATaaa                                                                                      >  2:1357788/1‑139 (MQ=255)                               tttttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACcg                                                                                   <  1:1090901/139‑1 (MQ=255)                                 tttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGTCGACCGCTTCGGCGCATAGGTTGAAATAAACcgcg                                                                                 >  2:219041/1‑139 (MQ=255)                                  ttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATaaa                                                                                      <  1:995117/133‑1 (MQ=255)                                  ttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATaaa                                                                                      >  2:995117/1‑133 (MQ=255)                                   tCATCGAGATGGCGAACCGTGTGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCa                                                                               <  1:1357788/139‑1 (MQ=255)                                       cGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATgg                                                                           <  2:88391/139‑1 (MQ=255)                                        gagaTGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGc                                                                          >  1:857040/1‑139 (MQ=255)                                             ggCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGcc                                                                     <  2:805056/139‑1 (MQ=255)                                                 aaCCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                                 <  1:306599/139‑1 (MQ=255)                                                    cGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                    <  2:349301/101‑1 (MQ=255)                                                    cGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                    >  1:349301/1‑101 (MQ=255)                                                      tGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCa                                                                               <  1:521216/120‑1 (MQ=255)                                                      tGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCa                                                                               >  2:521216/1‑120 (MQ=255)                                                      tGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATgg                                                                           <  2:1613396/124‑1 (MQ=255)                                                      tGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATgg                                                                           >  1:1613396/1‑124 (MQ=255)                                                      tGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                            <  1:555082/139‑1 (MQ=255)                                                       gCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCaa                                                           >  1:384091/1‑139 (MQ=255)                                                              ttGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTG‑CCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                <  2:398633/94‑1 (MQ=255)                                                              ttGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTG‑CCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                >  1:398633/1‑94 (MQ=255)                                                               tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTc                                                                                                          <  1:1515133/84‑1 (MQ=255)                                                               tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTc                                                                                                          >  2:1515133/1‑84 (MQ=255)                                                               tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                                 <  2:599765/125‑1 (MQ=255)                                                               tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                                 >  1:599765/1‑125 (MQ=255)                                                                gtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                  <  1:930796/139‑1 (MQ=255)                                                                gtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                  <  2:724/139‑1 (MQ=255)                                                                       catcatTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                    <  1:258339/82‑1 (MQ=255)                                                                       catcatTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                    >  2:258339/1‑82 (MQ=255)                                                                                 aaaaCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                            <  1:465800/112‑1 (MQ=255)                                                                                 aaaaCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                            >  2:465800/1‑112 (MQ=255)                                                                                    aCCACGCTGTTATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACcgcg                                                                                 <  2:972087/88‑1 (MQ=255)                                                                                    aCCACGCTGTTATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACcgcg                                                                                 >  1:972087/1‑88 (MQ=255)                                                                                    aCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGTCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCg                              <  1:219041/139‑1 (MQ=255)                                                                                    aCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTa                                          >  1:1253817/1‑127 (MQ=255)                                                                                    aCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTa                                          <  2:1253817/127‑1 (MQ=255)                                                                                    aCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCg                              >  1:1567765/1‑139 (MQ=255)                                                                                     ccACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCTCTTCGGCGCATAGGTTGAAATAAACCGCGCa                                                                               >  2:225111/1‑89 (MQ=255)                                                                                     ccACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCa                                                                               <  1:225111/89‑1 (MQ=255)                                                                                      cACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                    >  1:389343/1‑67 (MQ=255)                                                                                      cACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                    <  2:389343/67‑1 (MQ=255)                                                                                      cACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGcc                            >  2:449766/1‑139 (MQ=255)                                                                                         gCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATa                         <  1:449766/139‑1 (MQ=255)                                                                                              cATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATAc                                                      >  1:1342260/1‑105 (MQ=255)                                                                                              cATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATAc                                                      <  2:1342260/105‑1 (MQ=255)                                                                                                tctcGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAgcg                  <  1:1263022/139‑1 (MQ=255)                                                                                                     cccGTTTTACTGGTGCCCTGACTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATG‑GCGCCatat            >  2:1158270/1‑139 (MQ=255)                                                                                                         ttttACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                >  1:811207/1‑52 (MQ=255)                                                                                                         ttttACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                <  2:811207/52‑1 (MQ=255)                                                                                                         ttttACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                  >  2:184485/1‑98 (MQ=255)                                                                                                         ttttACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                  <  1:184485/98‑1 (MQ=255)                                                                                                         ttttACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGACCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCgg         >  1:1154115/1‑139 (MQ=255)                                                                                                          tttACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATgg                                                                           >  2:896033/1‑72 (MQ=255)                                                                                                          tttACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATgg                                                                           <  1:896033/72‑1 (MQ=255)                                                                                                              cTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTg    >  2:817894/1‑139 (MQ=255)                                                                                                                ggTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCt  <  1:487409/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                        AGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCT  >  NC_000913/746593‑746842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑