Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	829,972	C→T	M1M (GTG→ATG) † *224* (TAG→TAA)	ybhG ← ybiH ←	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor DUF1956 domain‑containing tetR family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	829,972	0	C	T	100.0%	209.0 / NA	64	M1M (GTG→ATG) † *224* (TAG→TAA)	ybhG ybiH	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor DUF1956 domain‑containing tetR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/31);  total (33/31)

TACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGG  >  NC_000913/829843‑830107                                                                                                                                  |                                                                                                                                        tACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTc                                                                                                                                >  1:463896/1‑139 (MQ=255)   cGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                                              <  2:1544843/139‑1 (MQ=255)    gAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCtt                                                                                                                             <  1:1400533/139‑1 (MQ=255)               ttGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCttt                                                                                                                            >  1:113720/1‑129 (MQ=255)               ttGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCttt                                                                                                                            <  2:113720/129‑1 (MQ=255)                   cATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGc                                                                                                              >  1:887408/1‑139 (MQ=255)                           gTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      <  1:1237280/139‑1 (MQ=255)                            tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATc                                                                                                                   >  1:745124/1‑125 (MQ=255)                            tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATc                                                                                                                   <  2:745124/125‑1 (MQ=255)                            tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATc                                                                                                        >  1:813433/1‑136 (MQ=255)                            tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATc                                                                                                        <  2:813433/136‑1 (MQ=255)                            tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAgg                                                                                                     >  2:293428/1‑139 (MQ=255)                            tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAgg                                                                                                     >  1:153649/1‑139 (MQ=255)                                    ttATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATg                                                                                             >  1:499383/1‑139 (MQ=255)                                          tGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                       <  2:293194/139‑1 (MQ=255)                                          tGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                       <  2:1449141/139‑1 (MQ=255)                                          tGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                       <  2:463896/139‑1 (MQ=255)                                          tGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                       <  1:1067646/139‑1 (MQ=255)                                            gcggcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGa                                                                                                 >  1:850059/1‑127 (MQ=255)                                            gcggcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGa                                                                                                 <  2:850059/127‑1 (MQ=255)                                            gcggcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                       <  1:652693/137‑1 (MQ=255)                                            gcggcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                       >  2:652693/1‑137 (MQ=255)                                                 tttGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATc                                                                                                                   >  1:151355/1‑104 (MQ=255)                                                 tttGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATc                                                                                                                   <  2:151355/104‑1 (MQ=255)                                                             aGTAGCCTCCGGCAACCACGGCGGCGAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTc                                                                                                   <  1:910493/108‑1 (MQ=255)                                                             aGTAGCCTCCGGCAACCACGGCGGCGAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTc                                                                                                   >  2:910493/1‑108 (MQ=255)                                                               tAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAAc                                                                                                                                  >  2:1152557/1‑75 (MQ=255)                                                               tAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAAc                                                                                                                                  <  1:1152557/75‑1 (MQ=255)                                                               tAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTc                                                                  >  1:260841/1‑139 (MQ=255)                                                                    tCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      >  2:1215081/1‑98 (MQ=255)                                                                    tCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      <  1:1215081/98‑1 (MQ=255)                                                                      cGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCttt                                                           >  1:1402184/1‑139 (MQ=255)                                                                        gCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATc                                                                                                                   >  1:348150/1‑81 (MQ=255)                                                                        gCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATc                                                                                                                   <  2:348150/81‑1 (MQ=255)                                                                        gCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGAt                                                                        >  2:1083583/1‑124 (MQ=255)                                                                        gCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGAt                                                                        <  1:1083583/124‑1 (MQ=255)                                                                               cggcggCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCg                                                  >  2:1488325/1‑139 (MQ=255)                                                                                  cggcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAAc                                               >  1:182265/1‑139 (MQ=255)                                                                                       aGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGt                                          >  1:1425846/1‑139 (MQ=255)                                                                                       aGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGt                                          <  2:182265/139‑1 (MQ=255)                                                                                          aCCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCa                                       >  1:796912/1‑139 (MQ=255)                                                                                          aCCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCa                                       >  1:55691/1‑139 (MQ=255)                                                                                            cACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                                     <  2:1402184/139‑1 (MQ=255)                                                                                            cACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                                     >  2:777378/1‑139 (MQ=255)                                                                                              cTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      >  1:870259/1‑72 (MQ=255)                                                                                              cTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      <  2:870259/72‑1 (MQ=255)                                                                                               tACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGt                                                                                                    >  2:184612/1‑73 (MQ=255)                                                                                               tACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGt                                                                                                    <  1:184612/73‑1 (MQ=255)                                                                                                 cccccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTa                                <  2:1302501/136‑1 (MQ=255)                                                                                                 ccGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTa                                >  2:1596387/1‑139 (MQ=255)                                                                                                 ccGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTa                                <  2:499383/139‑1 (MQ=255)                                                                                                   gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGa                                                                                                 >  2:1198689/1‑72 (MQ=255)                                                                                                   gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGa                                                                                                 <  1:1198689/72‑1 (MQ=255)                                                                                                   gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAAcgc                                             >  1:221603/1‑124 (MQ=255)                                                                                                   gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAAcgc                                             <  2:221603/124‑1 (MQ=255)                                                                                                   gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACg                              >  1:149203/1‑139 (MQ=255)                                                                                                    ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGt                             >  1:560405/1‑139 (MQ=255)                                                                                                            aTCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGt                                                                    >  2:562738/1‑92 (MQ=255)                                                                                                            aTCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGt                                                                    <  1:562738/92‑1 (MQ=255)                                                                                                             tCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAAtt                    <  2:153649/139‑1 (MQ=255)                                                                                                                  aCAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTc               <  2:1425846/139‑1 (MQ=255)                                                                                                                      gTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGAc                                                                                           >  1:862868/1‑59 (MQ=255)                                                                                                                      gTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGAc                                                                                           <  2:862868/59‑1 (MQ=255)                                                                                                                               tcaTTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACgg  <  2:796912/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        TACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGG  >  NC_000913/829843‑830107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑