Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	86,472	A→G	V281V (GTA→GTG)	ilvI →	acetolactate synthase 3 large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	86,472	0	A	G	100.0%	293.9 / NA	81	V281V (GTA→GTG)	ilvI	acetolactate synthase 3 large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/44);  total (37/44)

TGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCC  >  NC_000913/86340‑86605                                                                                                                                     |                                                                                                                                      tgttTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGAttt                                                                                                                                 <  2:809251/139‑1 (MQ=255)   tttGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTtga                                                                                                                               <  1:725427/139‑1 (MQ=255)    ttGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTtgat                                                                                                                              <  2:716206/139‑1 (MQ=255)                  ggCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGaa                                                                                                                >  2:550643/1‑139 (MQ=255)                   gCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAAc                                                                                                               >  1:1281856/1‑139 (MQ=255)                    cTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACa                                                                                                              <  2:339217/139‑1 (MQ=255)                      gggggCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAAt                                                                                                            >  2:1003805/1‑139 (MQ=255)                      gggggCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAAt                                                                                                            >  2:1325313/1‑139 (MQ=255)                        gggCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGa                                                                                                                                    >  1:885521/1‑113 (MQ=255)                        gggCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGa                                                                                                                                    <  2:885521/113‑1 (MQ=255)                        gggCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCt                                                                                                          >  1:728026/1‑139 (MQ=255)                            gTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCa                                                                                                      <  2:310160/139‑1 (MQ=255)                             tttCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCaa                                                                                                     >  1:536758/1‑139 (MQ=255)                             tttCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCaa                                                                                                     >  1:1371126/1‑139 (MQ=255)                                       cGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGccc                                                                                           <  1:1325313/139‑1 (MQ=255)                                       cGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGccc                                                                                           >  2:489284/1‑139 (MQ=255)                                       cGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGccc                                                                                           <  2:1371126/139‑1 (MQ=255)                                        gCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCa                                                                                          <  2:926178/139‑1 (MQ=255)                                        gCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCa                                                                                          <  2:1281856/139‑1 (MQ=255)                                          aTCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCaaa                                                                                        <  1:1223785/139‑1 (MQ=255)                                          aTCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCaaa                                                                                        <  1:348800/139‑1 (MQ=255)                                          aTCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCaaa                                                                                        <  1:873361/139‑1 (MQ=255)                                               cAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCa                                                                                   <  2:535858/139‑1 (MQ=255)                                               cAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCa                                                                                   <  2:728026/139‑1 (MQ=255)                                               cAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATCACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCa                                                                                   <  1:297576/139‑1 (MQ=255)                                                 ggCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACt                                                                                 <  1:991946/139‑1 (MQ=255)                                                   cACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGt                                                                               <  2:878803/139‑1 (MQ=255)                                                    aCTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGtt                                                                              <  2:536758/139‑1 (MQ=255)                                                      tGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCa                                                                                                      >  2:1460108/1‑113 (MQ=255)                                                      tGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCa                                                                                                      <  1:1460108/113‑1 (MQ=255)                                                       gggCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTg                                                                           <  1:1043113/139‑1 (MQ=255)                                                        ggCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGtt                                                                              >  2:480494/1‑135 (MQ=255)                                                        ggCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGtt                                                                              <  1:480494/135‑1 (MQ=255)                                                            tGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCatat                                                                      >  2:480055/1‑139 (MQ=255)                                                            tGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCatat                                                                      <  1:1003805/139‑1 (MQ=255)                                                             gCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCt                                                                            >  1:1191662/1‑132 (MQ=255)                                                             gCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCt                                                                            <  2:1191662/132‑1 (MQ=255)                                                                       cACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATc                                                           >  2:1176285/1‑139 (MQ=255)                                                                                 aCGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATc                                                                                                           >  2:1492910/1‑81 (MQ=255)                                                                                 aCGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATc                                                                                                           <  1:1492910/81‑1 (MQ=255)                                                                                 aCGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCAt                                                 >  1:1128277/1‑139 (MQ=255)                                                                                     aGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCt                                             <  1:480055/139‑1 (MQ=255)                                                                                      gCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAacg                                                                                                                     >  2:1174847/1‑66 (MQ=255)                                                                                      gCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAacg                                                                                                                     <  1:1174847/66‑1 (MQ=255)                                                                                      gCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAac                                                                                                                      >  1:762136/1‑65 (MQ=255)                                                                                      gCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAac                                                                                                                      <  2:762136/65‑1 (MQ=255)                                                                                      gCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCa                                                                                   >  1:1123129/1‑100 (MQ=255)                                                                                      gCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCa                                                                                   <  2:1123129/100‑1 (MQ=255)                                                                                      gCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATAtt                                                               <  2:1188373/120‑1 (MQ=255)                                                                                      gCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATAtt                                                               >  1:1188373/1‑120 (MQ=255)                                                                                        cAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTaaa                                          >  1:508055/1‑139 (MQ=255)                                                                                         aaTATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATc                                                                                                           <  1:1574598/73‑1 (MQ=255)                                                                                         aaTATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATc                                                                                                           >  2:1574598/1‑73 (MQ=255)                                                                                          atatGACGATGCATAACGCGGATGTGATTTTCGCTGTCGGGGTGCGATTTGATGACTGTACGACGAACAATc                                                                                                           <  1:1078486/72‑1 (MQ=255)                                                                                          atatGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATc                                                                                                           >  2:1078486/1‑72 (MQ=255)                                                                                                     cATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATc                                                                                                           >  1:340741/1‑61 (MQ=255)                                                                                                     cATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATc                                                                                                           <  2:340741/61‑1 (MQ=255)                                                                                                     cATAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCgg                             >  2:346293/1‑139 (MQ=255)                                                                                                      aTAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGa                            >  1:681067/1‑139 (MQ=255)                                                                                                      aTAACGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGa                            <  1:346293/139‑1 (MQ=255)                                                                                                        aaCGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAac                                                                                                                      <  2:1543114/47‑1 (MQ=255)                                                                                                        aaCGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAac                                                                                                                      >  1:1543114/1‑47 (MQ=255)                                                                                                         aCGCGGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGatat                         >  1:271018/1‑139 (MQ=255)                                                                                                          cgcgGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATAtt                                                               >  2:1518943/1‑100 (MQ=255)                                                                                                          cgcgGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATAtt                                                               <  1:1518943/100‑1 (MQ=255)                                                                                                          cgcgGATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATc                        >  1:1000914/1‑139 (MQ=255)                                                                                                             ggATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCaaa                                                                                        <  2:1136690/72‑1 (MQ=255)                                                                                                             ggATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGAAGAACAATCTGGCAAAGTACTGCCCaaa                                                                                        >  1:1136690/1‑72 (MQ=255)                                                                                                              gATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATcc                       >  2:965428/1‑136 (MQ=255)                                                                                                              gATGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATcc                       <  1:965428/136‑1 (MQ=255)                                                                                                               aTGTGATTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGAt                   >  2:48334/1‑139 (MQ=255)                                                                                                                 gtgATTTTCGCCGTCGGGGTGCGA‑TTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCa                                                                                   >  2:1205152/1‑72 (MQ=255)                                                                                                                 gtgATTTTCGCCGTCGGGGTGCGA‑TTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCa                                                                                   <  1:1205152/72‑1 (MQ=255)                                                                                                                    aTTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCa                                                                                                      <  1:95676/51‑1 (MQ=255)                                                                                                                    aTTTTCGCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCa                                                                                                      >  2:95676/1‑51 (MQ=255)                                                                                                                          gCCGTCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATg        <  1:489284/139‑1 (MQ=255)                                                                                                                              tCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTg                                                                           >  2:211702/1‑68 (MQ=255)                                                                                                                              tCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTg                                                                           <  1:211702/68‑1 (MQ=255)                                                                                                                              tCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCt                                             >  2:1368599/1‑98 (MQ=255)                                                                                                                              tCGGGGTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCt                                             <  1:1368599/98‑1 (MQ=255)                                                                                                                                ggggTGCGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGcc  <  2:681067/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      TGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCC  >  NC_000913/86340‑86605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑