Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,208,517 C→T P129L (CCT→CTT) 
*201* (TAG→TAA
tfaP →
tfaE ←
e14 prophage, uncharacterized protein
e14 prophage, putative tail fiber assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,208,5170CT100.0% 209.2 / NA 68P129L (CCT→CTT) 
*201* (TAG→TAA
tfaP
tfaE
e14 prophage, uncharacterized protein
e14 prophage, putative tail fiber assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/28);  total (40/28)

TAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCT  >  NC_000913/1208383‑1208647
                                                                                                                                      |                                                                                                                                  
tAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACg                                                                                                                                >  1:1199319/1‑139 (MQ=255)
tAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACg                                                                                                                                >  2:327391/1‑139 (MQ=255)
  gCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGcc                                                                                                                              <  2:1199319/139‑1 (MQ=255)
      aCAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                          <  1:745223/139‑1 (MQ=255)
      aCAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                          >  2:73178/1‑139 (MQ=255)
          tGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAg                                                                                                                      >  2:1379661/1‑139 (MQ=255)
          tGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAg                                                                                                                      >  2:1018221/1‑139 (MQ=255)
          tGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAg                                                                                                                      >  2:615253/1‑139 (MQ=255)
                ggTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGttaggc                                                                                                                >  1:338252/1‑135 (MQ=255)
                          cATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACAt                                                                                                     >  2:293758/1‑139 (MQ=255)
                          cATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACAt                                                                                                     >  2:143691/1‑139 (MQ=255)
                          cATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACAt                                                                                                     >  2:1178704/1‑139 (MQ=255)
                          cATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACAt                                                                                                     >  2:808376/1‑139 (MQ=255)
                                gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                               <  1:327391/139‑1 (MQ=255)
                                gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                               <  1:808376/139‑1 (MQ=255)
                                gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                               <  1:503232/139‑1 (MQ=255)
                                  aGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGcgcg                                                                                             <  1:1379661/139‑1 (MQ=255)
                                  aGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGcgcg                                                                                             <  1:985386/139‑1 (MQ=255)
                                    aCTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGt                                                                                           <  1:293758/139‑1 (MQ=255)
                                    aCTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGt                                                                                           >  1:1214520/1‑139 (MQ=255)
                                         aagaagATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTgg                                                                                      >  2:1571106/1‑139 (MQ=255)
                                                tGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGt                                                                               >  2:155308/1‑139 (MQ=255)
                                                tGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGt                                                                               >  1:55589/1‑139 (MQ=255)
                                                    cTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGcc                                                                           >  2:596903/1‑139 (MQ=255)
                                                      gAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                          <  1:1546674/91‑1 (MQ=255)
                                                      gAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                          >  2:1546674/1‑91 (MQ=255)
                                                        aaCAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCa                                                                       <  1:73178/139‑1 (MQ=255)
                                                         aCAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                      <  1:143691/139‑1 (MQ=255)
                                                             tataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCg                                                                                                  >  1:1512138/1‑107 (MQ=255)
                                                             tataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCg                                                                                                  <  2:1512138/107‑1 (MQ=255)
                                                                  tttGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAAt                                                             >  2:73111/1‑139 (MQ=255)
                                                                   ttGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTGCGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                            <  1:1513544/139‑1 (MQ=255)
                                                                   ttGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                            <  1:1385732/139‑1 (MQ=255)
                                                                   ttGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                            <  1:155308/139‑1 (MQ=255)
                                                                     gtgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTa                                                          >  2:896323/1‑139 (MQ=255)
                                                                       ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGc                                                                                                                               >  2:13421/1‑69 (MQ=255)
                                                                       ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGc                                                                                                                               <  1:13421/69‑1 (MQ=255)
                                                                       ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                      >  1:774637/1‑125 (MQ=255)
                                                                       ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                      <  2:774637/125‑1 (MQ=255)
                                                                       ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAAt                                                        >  2:720672/1‑139 (MQ=255)
                                                                        gCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATc                                                       <  2:1145988/139‑1 (MQ=255)
                                                                        gCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATc                                                       >  2:417258/1‑139 (MQ=255)
                                                                        gCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATc                                                       >  1:29012/1‑139 (MQ=255)
                                                                            gATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGc                                                   >  1:513872/1‑139 (MQ=255)
                                                                              ttATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCAAGcac                                                 <  1:1018221/139‑1 (MQ=255)
                                                                                   tGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGt                                            >  2:500/1‑139 (MQ=255)
                                                                                      aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGcgcg                                                                                             <  2:330014/87‑1 (MQ=255)
                                                                                      aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGcgcg                                                                                             >  1:330014/1‑87 (MQ=255)
                                                                                            tGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGtta                                                                                                                   >  1:858120/1‑59 (MQ=255)
                                                                                            tGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGtta                                                                                                                   <  2:858120/60‑2 (MQ=255)
                                                                                                   ggTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTc                            >  2:910503/1‑139 (MQ=255)
                                                                                                            aTCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                               <  2:199693/63‑1 (MQ=14)
                                                                                                            aTCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                               >  1:199693/1‑63 (MQ=11)
                                                                                                              ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTct                           <  2:152886/129‑1 (MQ=255)
                                                                                                              ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTct                           >  1:152886/1‑129 (MQ=255)
                                                                                                              ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                   <  2:560898/137‑1 (MQ=255)
                                                                                                              ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                   >  1:560898/1‑137 (MQ=255)
                                                                                                              ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCa                 >  2:1212130/1‑139 (MQ=255)
                                                                                                              ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCa                 >  1:587346/1‑139 (MQ=255)
                                                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                               <  1:124225/57‑1 (MQ=11)
                                                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                               >  2:124225/1‑57 (MQ=11)
                                                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGGGTCAGttt             >  1:978570/1‑139 (MQ=255)
                                                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt             >  1:450402/1‑139 (MQ=255)
                                                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt             >  1:1510810/1‑139 (MQ=255)
                                                                                                                    cgcCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                      >  1:181498/1‑80 (MQ=11)
                                                                                                                    cgcCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                      <  2:181498/80‑1 (MQ=11)
                                                                                                                          atatTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCgg     <  1:615253/139‑1 (MQ=255)
                                                                                                                             ttGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCt  <  1:417258/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
TAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCT  >  NC_000913/1208383‑1208647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: