Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,403,059	A→G	I66T (ATT→ACT)	abgB ←	p‑aminobenzoyl‑glutamate hydrolase, B subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,403,059	0	A	G	100.0%	190.9 / NA	53	I66T (ATT→ACT)	abgB	p‑aminobenzoyl‑glutamate hydrolase, B subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/27);  total (26/27)

CAACCGTGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAATAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATA  >  NC_000913/1402930‑1403193                                                                                                                                  |                                                                                                                                       cAACCGTGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCAt                                                                                                                               >  1:1115188/1‑139 (MQ=255) cAACCGTGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCAt                                                                                                                               <  1:1174565/139‑1 (MQ=255)  aaCCGTGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCAtt                                                                                                                              >  1:621105/1‑139 (MQ=255)   aCCGTGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCAttt                                                                                                                             >  1:482701/1‑139 (MQ=255)              tttCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGcc                                                                                                                  <  2:1188868/139‑1 (MQ=255)               ttCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCt                                                                                                                 <  2:464778/139‑1 (MQ=255)                     gggCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGat                                                                                                                        >  2:18692/1‑126 (MQ=255)                     gggCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGat                                                                                                                        <  1:18692/126‑1 (MQ=255)                       gCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCg                                                                                                         <  2:510715/139‑1 (MQ=255)                       gCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCg                                                                                                         >  1:810519/1‑139 (MQ=255)                         gTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCggg                                                                                                       <  2:912785/139‑1 (MQ=255)                                        tGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGat                                                                                                                        <  1:559646/107‑1 (MQ=255)                                        tGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGat                                                                                                                        >  2:559646/1‑107 (MQ=255)                                          cgcgcAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCa                                                                                      <  1:1226987/139‑1 (MQ=255)                                            cgcAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGcc                                                                                                         <  1:1007052/118‑2 (MQ=255)                                            cgcAACCTGCTTGCTGAATTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCg                                                                                                         >  2:1007052/1‑118 (MQ=255)                                                       tgctGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCg                                                                         <  2:256114/139‑1 (MQ=255)                                                            aCTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGcc                                                                    >  2:1323827/1‑139 (MQ=255)                                                            aCTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTGTGCCTTGACGAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGcc                                                                    <  1:85447/139‑1 (MQ=255)                                                             cTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCa                                                                   >  2:936768/1‑139 (MQ=255)                                                             cTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCa                                                                   >  2:19229/1‑139 (MQ=255)                                                               tAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCAGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCg                                                                                                         >  1:66786/1‑99 (MQ=255)                                                               tAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCAGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCg                                                                                                         <  2:66786/99‑1 (MQ=255)                                                                      gCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCg                                                          <  2:621105/139‑1 (MQ=255)                                                                      gCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCg                                                          <  2:1115188/139‑1 (MQ=255)                                                                           ggCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGcc                                                                    <  1:893463/124‑1 (MQ=255)                                                                           ggCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGcc                                                                    >  2:893463/1‑124 (MQ=255)                                                                           ggCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGc                                                                     <  1:1194873/123‑1 (MQ=255)                                                                           ggCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGc                                                                     >  2:1194873/1‑123 (MQ=255)                                                                                cATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGaa                                                <  2:482701/139‑1 (MQ=255)                                                                                 atatTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTaaa                                                                                                                                    >  1:124705/1‑54 (MQ=255)                                                                                 atatTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTaaa                                                                                                                                    <  2:124705/54‑1 (MQ=255)                                                                                          cagcagGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCaaaa                                      >  2:1371723/1‑139 (MQ=255)                                                                                               gggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGcc                                                                          >  1:261728/1‑98 (MQ=255)                                                                                               gggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGcc                                                                          <  2:261728/98‑1 (MQ=255)                                                                                               gggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCg                                                                         <  1:270072/99‑1 (MQ=255)                                                                                               gggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCg                                                                         >  2:270072/1‑99 (MQ=255)                                                                                                ggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGGCCAGAACTCTTCAAAACGTGtt                                >  1:1513313/1‑139 (MQ=255)                                                                                                ggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGtt                                >  2:952231/1‑139 (MQ=255)                                                                                                ggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGtt                                >  1:64655/1‑139 (MQ=255)                                                                                                ggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGtt                                >  1:1363841/1‑139 (MQ=255)                                                                                                      aaCCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGga                          <  2:563535/139‑1 (MQ=255)                                                                                                       aCCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgat                         <  2:810519/139‑1 (MQ=255)                                                                                                           gTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCt                                                                                                                 <  1:661029/47‑1 (MQ=255)                                                                                                           gTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCt                                                                                                                 >  2:661029/1‑47 (MQ=255)                                                                                                               gCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAg                 >  2:452302/1‑139 (MQ=255)                                                                                                                     aCCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGcc                                                                    >  2:636683/1‑82 (MQ=255)                                                                                                                     aCCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGcc                                                                    <  1:636683/82‑1 (MQ=255)                                                                                                                          aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGtt                                                                                                            >  2:633822/1‑37 (MQ=255)                                                                                                                          aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGtt                                                                                                            <  1:633822/37‑1 (MQ=255)                                                                                                                          aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGc      <  1:1323827/139‑1 (MQ=255)                                                                                                                              aGCAGTAAAGGCATTTGGGATATTGCTTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGGCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAata  <  1:374354/139‑1 (MQ=255)                                                                                                                              aGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAata  >  2:1428359/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                       CAACCGTGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAATAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATA  >  NC_000913/1402930‑1403193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑