Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,521,048 A→G V673A (GTC→GCC)  yncD ← putative iron outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,521,0480AG98.1% 365.4 / NA 103V673A (GTC→GCC) yncDputative iron outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (49/52);  total (50/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

GCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGCCG  >  NC_000913/1520912‑1521180
                                                                                                                                        |                                                                                                                                    
gCAATACAATCGTTAAAGTAAATGCCCGGCAAGTACCGGGCATCAAGGTAATTACTCAAAGCTCCACGCAATATCCATGCCGACACCATAATTTCTGCCGGTCGAACGTTCGTAATATCGCCCGTTTGACTCATTGACa                                                                                                                                    <  1:1095209/139‑1 (MQ=255)
    tACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGa                                                                                                                                <  1:805479/139‑1 (MQ=255)
    tACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGa                                                                                                                                <  2:970107/139‑1 (MQ=255)
            ttAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCa                                                                                                                        >  2:249015/1‑139 (MQ=255)
              aaaGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAAc                                                                                                                      >  1:1265391/1‑139 (MQ=255)
              aaaGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAAc                                                                                                                      >  1:556768/1‑139 (MQ=255)
                    aaTGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCa                                                                                                                        >  2:1457276/1‑131 (MQ=255)
                    aaTGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCa                                                                                                                        <  1:1457276/131‑1 (MQ=255)
                     aTGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCt                                                                                                               >  2:1327809/1‑139 (MQ=255)
                       gCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCttt                                                                                                             >  1:1446558/1‑139 (MQ=255)
                            gCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCCTTGACAATGACAGAAAAAACGTATTCTTTATCGa                                                                                                        >  1:17424/1‑139 (MQ=255)
                            gCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTAtt                                                                                                                 >  2:678593/1‑130 (MQ=255)
                                       gCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAAc                                                                                                                      >  1:286383/1‑114 (MQ=255)
                                       gCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAAc                                                                                                                      <  2:286383/114‑1 (MQ=255)
                                       gCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCg                                                                                             >  2:112228/1‑139 (MQ=255)
                                       gCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCg                                                                                             >  2:292091/1‑139 (MQ=255)
                                         aTCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGac                                                                                           >  2:145533/1‑139 (MQ=255)
                                            aaGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACg                                                                                        >  2:1248644/1‑139 (MQ=255)
                                                        cAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                            <  2:1446558/139‑1 (MQ=255)
                                                         aaaTCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATcc                                                                           >  2:1521074/1‑139 (MQ=255)
                                                                cACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGaa                                                                                                                           >  2:176165/1‑84 (MQ=255)
                                                                cACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGaa                                                                                                                           <  1:176165/84‑1 (MQ=255)
                                                                 aCGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTaa                                                                   >  2:131842/1‑139 (MQ=255)
                                                                 aCGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTaa                                                                   >  2:877224/1‑139 (MQ=255)
                                                                   gCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAAt                                                                 <  2:1265391/139‑1 (MQ=255)
                                                                   gCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAAt                                                                 <  1:89932/139‑1 (MQ=255)
                                                                       tatTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAAcc                                                                                                                         >  2:1436347/1‑79 (MQ=255)
                                                                       tatTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAAcc                                                                                                                         <  1:1436347/79‑1 (MQ=255)
                                                                       tatTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAAc                                                                                                                      >  2:1456750/1‑82 (MQ=255)
                                                                       tatTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAAc                                                                                                                      <  1:1456750/82‑1 (MQ=255)
                                                                        atTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                            <  1:660669/123‑1 (MQ=255)
                                                                        atTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCGTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                            >  2:660669/1‑123 (MQ=255)
                                                                         ttCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGt                                                           >  2:736247/1‑139 (MQ=255)
                                                                           cATGCGGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTaa                                                         <  1:551421/139‑1 (MQ=255)
                                                                            aTGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                        <  1:877224/139‑1 (MQ=255)
                                                                            aTGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                        <  1:1248644/139‑1 (MQ=255)
                                                                            aTGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                        <  2:763894/139‑1 (MQ=255)
                                                                            aTGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                        <  1:450572/139‑1 (MQ=255)
                                                                            aTGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                        <  1:249015/139‑1 (MQ=255)
                                                                             tGCCGACTCCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACATAACCAACGTATTCTTTATCGTATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAtt                                                       <  2:17424/139‑1 (MQ=255)
                                                                             tGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                          <  2:854104/120‑1 (MQ=255)
                                                                             tGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                          >  1:854104/1‑120 (MQ=255)
                                                                             tGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAtt                                                       <  1:134987/139‑1 (MQ=255)
                                                                              gCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGa                                                                                       <  2:138157/106‑1 (MQ=255)
                                                                              gCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGa                                                                                       >  1:138157/1‑106 (MQ=255)
                                                                               ccGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGt                                                     >  1:1264588/1‑139 (MQ=255)
                                                                                 gACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGaa                                                                                                                           >  1:915265/1‑67 (MQ=255)
                                                                                 gACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGaa                                                                                                                           <  2:915265/67‑1 (MQ=255)
                                                                                 gACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAAt                                                                                                      >  2:392728/1‑88 (MQ=255)
                                                                                 gACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAAt                                                                                                      <  1:392728/88‑1 (MQ=255)
                                                                                 gACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTAt                                                   >  1:811078/1‑139 (MQ=255)
                                                                                 gACACCATAATTTCGTCCGGGCGAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                      <  1:743238/103‑1 (MQ=255)
                                                                                 gACACCATAATTTCGTCCGGGCGAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                      >  2:743238/1‑103 (MQ=255)
                                                                                   cacCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCt                                                                                                               >  2:734495/1‑77 (MQ=255)
                                                                                   cacCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCt                                                                                                               <  1:734495/77‑1 (MQ=255)
                                                                                   cacCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTg                                                      <  2:719354/134‑1 (MQ=255)
                                                                                   cacCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTg                                                      >  1:719354/1‑134 (MQ=255)
                                                                                      cATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATa                                                                                                     <  1:1113893/84‑1 (MQ=255)
                                                                                      cATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATa                                                                                                     >  2:1113893/1‑84 (MQ=255)
                                                                                      cATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACg                                                                                        >  2:72090/1‑97 (MQ=255)
                                                                                      cATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACg                                                                                        <  1:72090/97‑1 (MQ=255)
                                                                                        tAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATAcc                                            <  2:1264588/139‑1 (MQ=255)
                                                                                              cGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGa                                      >  2:783862/1‑139 (MQ=255)
                                                                                                tCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                            <  1:864527/99‑1 (MQ=255)
                                                                                                tCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                            <  1:276651/99‑1 (MQ=255)
                                                                                                tCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                            >  2:864527/1‑99 (MQ=255)
                                                                                                tCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                            >  2:276651/1‑99 (MQ=255)
                                                                                                tCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                        <  2:507430/119‑1 (MQ=255)
                                                                                                tCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                        >  1:507430/1‑119 (MQ=255)
                                                                                                   gggCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAg                                 <  1:148294/139‑1 (MQ=255)
                                                                                                    ggCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                          >  1:230769/1‑97 (MQ=255)
                                                                                                    ggCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                          <  1:1578021/97‑1 (MQ=255)
                                                                                                    ggCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                          <  2:230769/97‑1 (MQ=255)
                                                                                                     gCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTGTCAACACGACCAAATAAATCca                                                                          >  2:987754/1‑96 (MQ=255)
                                                                                                     gCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                          <  1:987754/96‑1 (MQ=255)
                                                                                                     gCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGcc                               <  2:556768/139‑1 (MQ=255)
                                                                                                          ggTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCcgacga                          <  2:811078/139‑1 (MQ=255)
                                                                                                          ggTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCcgacga                          <  1:145533/139‑1 (MQ=255)
                                                                                                          ggTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCcgacga                          <  2:823789/139‑1 (MQ=255)
                                                                                                          ggTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCcgacga                          <  1:783862/139‑1 (MQ=255)
                                                                                                              cGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                      >  2:1596681/1‑75 (MQ=255)
                                                                                                              cGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                      <  1:184081/75‑1 (MQ=255)
                                                                                                              cGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                      <  1:1596681/75‑1 (MQ=255)
                                                                                                              cGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                      >  2:184081/1‑75 (MQ=255)
                                                                                                                     tCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGAc               >  1:1418838/1‑139 (MQ=255)
                                                                                                                     tCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGAc               >  1:861870/1‑139 (MQ=255)
                                                                                                                      cGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGAc               <  2:176933/138‑1 (MQ=255)
                                                                                                                      cGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGAc               >  1:176933/1‑138 (MQ=255)
                                                                                                                      cGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACg              >  2:965025/1‑139 (MQ=255)
                                                                                                                      cGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAAACCACAGTTAAATTGTGGTAATTGTATGTATACCCGGTGAATAAGCCGACGAGAGTATAAGACg              >  2:1011448/1‑139 (MQ=255)
                                                                                                                        cccGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTa                                                                                                            >  1:645332/1‑43 (MQ=255)
                                                                                                                        cccGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTa                                                                                                            <  2:645332/43‑1 (MQ=255)
                                                                                                                        cccGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                          <  2:1048887/77‑1 (MQ=255)
                                                                                                                        cccGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                          >  1:1048887/1‑77 (MQ=255)
                                                                                                                        cccGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCcgac                            >  2:809218/1‑123 (MQ=255)
                                                                                                                        cccGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCcgac                            <  1:809218/123‑1 (MQ=255)
                                                                                                                               gACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTg     <  1:131842/139‑1 (MQ=255)
                                                                                                                                aCTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGc    <  1:532888/139‑1 (MQ=255)
                                                                                                                                 cTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGcc   >  2:1350186/1‑139 (MQ=255)
                                                                                                                                  tCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGCCg  <  2:1418838/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
GCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGCCG  >  NC_000913/1520912‑1521180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: