Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,548,360	C→T	D320D (GAC→GAT)	fdnG →	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,548,360	0	C	T	100.0%	185.2 / NA	57	D320D (GAC→GAT)	fdnG	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/25);  total (32/25)

GGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTG  >  NC_000913/1548232‑1548492                                                                                                                                 |                                                                                                                                     ggTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGt                                                                                                                                   <  2:22170/132‑1 (MQ=255) ggTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGt                                                                                                                                   >  1:22170/1‑132 (MQ=255)       gACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCt                                                                                                                      >  1:984348/1‑139 (MQ=255)        aCATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTa                                                                                                                     <  2:360683/139‑1 (MQ=255)            tACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTacgac                                                                                                                 >  2:940761/1‑139 (MQ=255)            tACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTacgac                                                                                                                 >  1:831364/1‑139 (MQ=255)              cGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGc                                                                                                               <  1:7706/139‑1 (MQ=255)                  ccTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTa                                                                                                                     <  1:836052/129‑1 (MQ=255)                  ccTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTa                                                                                                                     >  2:836052/1‑129 (MQ=255)                  ccTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaa                                                                                                           >  2:728474/1‑139 (MQ=255)                    tGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaaaa                                                                                                         >  2:1228288/1‑139 (MQ=255)                    tGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACATTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaaaa                                                                                                         >  2:753658/1‑139 (MQ=255)                      tCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAAc                                                                                                       >  2:1181198/1‑139 (MQ=255)                          gCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCa                                                                                                   <  2:831364/139‑1 (MQ=255)                          gCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCa                                                                                                   <  2:1398396/139‑1 (MQ=255)                             ttttGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATa                                                                                                >  1:970726/1‑139 (MQ=255)                               ttGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGt                                                                                                                                   >  1:36425/1‑102 (MQ=255)                               ttGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGt                                                                                                                                   <  2:36425/102‑1 (MQ=255)                                tGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGa                                                                                             >  2:569065/1‑139 (MQ=255)                                tGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGa                                                                                             >  1:1440346/1‑139 (MQ=255)                                     tACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAAt                                                                                        <  1:1177977/139‑1 (MQ=255)                                         tGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc                                                                                    <  1:940761/139‑1 (MQ=255)                                                 aacaacAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCa                                                                                                                            >  2:1153964/1‑91 (MQ=255)                                                 aacaacAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACt                                                                            >  1:110557/1‑139 (MQ=255)                                                 aacaacAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACt                                                                            >  1:1339595/1‑139 (MQ=255)                                                 aacaacAAAATCAAAGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCa                                                                                                                            <  1:1153964/91‑1 (MQ=255)                                                      caaAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAg                                                                       >  2:1598101/1‑139 (MQ=255)                                                                    aaTACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc                                                                                    <  1:487748/112‑1 (MQ=255)                                                                    aaTACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc                                                                                    >  2:487748/1‑112 (MQ=255)                                                                     aTACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCa                                                                                                                            <  1:401681/71‑1 (MQ=255)                                                                     aTACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCa                                                                                                                            >  2:401681/1‑71 (MQ=255)                                                                            aaGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGa                                                 <  1:1228288/139‑1 (MQ=255)                                                                              gCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGaaa                                               >  1:581892/1‑139 (MQ=255)                                                                                aTTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCa                                                                                                   >  2:242278/1‑85 (MQ=255)                                                                                aTTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCa                                                                                                   <  1:242278/85‑1 (MQ=255)                                                                                  tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCaa                                                                                                  >  1:599453/1‑84 (MQ=255)                                                                                  tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCaa                                                                                                  <  2:599453/84‑1 (MQ=255)                                                                                  tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCg                                                                   <  1:14868/115‑1 (MQ=255)                                                                                  tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCg                                                                   >  2:14868/1‑115 (MQ=255)                                                                                     acCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATTGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATg                                        <  2:984348/139‑1 (MQ=255)                                                                                         aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAAt                                                                                        <  2:531746/87‑1 (MQ=255)                                                                                         aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAAt                                                                                        >  1:531746/1‑87 (MQ=255)                                                                                         aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAac                                    >  2:1533294/1‑139 (MQ=255)                                                                                          cGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAaca                                   <  1:1181198/139‑1 (MQ=255)                                                                                                ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAAcg                                             <  1:185105/123‑1 (MQ=255)                                                                                                ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAAcg                                             >  2:185105/1‑123 (MQ=255)                                                                                                       gTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgc                      >  1:379458/1‑139 (MQ=255)                                                                                                        tGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg                     <  2:970726/139‑1 (MQ=255)                                                                                                          cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCgcgc                    <  2:530923/138‑1 (MQ=255)                                                                                                          cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCgcgc                    >  1:530923/1‑138 (MQ=255)                                                                                                          cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCt                   >  1:692393/1‑139 (MQ=255)                                                                                                            tgatgaTTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCtgt                 >  1:329453/1‑139 (MQ=255)                                                                                                                gatTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCtgtgtgt             <  2:1162185/139‑1 (MQ=255)                                                                                                                    ttGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGaa         <  1:1533294/139‑1 (MQ=255)                                                                                                                    ttGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGaa         <  2:1605292/139‑1 (MQ=255)                                                                                                                          tCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACctgct   >  2:605137/1‑139 (MQ=255)                                                                                                                           cGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACctgctg  >  1:1084028/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                     GGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTG  >  NC_000913/1548232‑1548492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑