Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,557,112 C→T *309* (TAG→TAA)  ddpF ← D,D‑dipeptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,557,1120CT100.0% 151.4 / NA 47*309* (TAG→TAA) ddpFD,D‑dipeptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/20);  total (27/20)

GAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTT  >  NC_000913/1556981‑1557241
                                                                                                                                   |                                                                                                                                 
gAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg                                                                                                                            <  1:572558/139‑1 (MQ=255)
      aaaagcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGcgcccgcc                                                                                                                      >  1:96760/1‑139 (MQ=255)
      aaaagcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGcgcccgcc                                                                                                                      >  2:159894/1‑139 (MQ=255)
       aaagcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg                                                                                                                            <  1:1367018/132‑1 (MQ=255)
       aaagcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg                                                                                                                            >  2:1367018/1‑132 (MQ=255)
             ggATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg                                                                                                               >  2:362957/1‑139 (MQ=255)
                            cAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc                                                                                                <  1:271334/139‑1 (MQ=255)
                             aGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCATTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACgtccgtcc                                                                                               <  2:1561725/139‑1 (MQ=255)
                              ggTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGtcct                                                                                              <  1:1267350/139‑1 (MQ=255)
                                  cTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCt                                                                                          <  2:96760/139‑1 (MQ=255)
                                   tGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg                                                                                                                            >  2:1451461/1‑104 (MQ=255)
                                   tGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg                                                                                                                            <  1:1451461/104‑1 (MQ=255)
                                     aaaGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTAt                                                                                       >  2:181688/1‑139 (MQ=255)
                                     aaaGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTAt                                                                                       >  2:542714/1‑139 (MQ=255)
                                       aGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTg                                                                                     <  1:362957/139‑1 (MQ=255)
                                          ggAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTa                                                                                  >  2:1573328/1‑139 (MQ=255)
                                          ggAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTa                                                                                  >  2:116502/1‑139 (MQ=255)
                                            aaaTTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAAt                                                                                <  1:181688/139‑1 (MQ=255)
                                                    cTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                        <  1:116502/139‑1 (MQ=255)
                                                      ggATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCAc                                                                                                                 >  1:230662/1‑96 (MQ=255)
                                                      ggATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCAc                                                                                                                 <  2:230662/96‑1 (MQ=255)
                                                          tACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTc                                                                  >  1:3423/1‑139 (MQ=255)
                                                                 tGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCtt                                                                                         <  2:923051/109‑1 (MQ=255)
                                                                 tGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCg                                                           >  2:365867/1‑139 (MQ=255)
                                                                 tGAAATCGTAAAGCATTACCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACATCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCtt                                                                                         >  1:923051/1‑109 (MQ=255)
                                                                     aTCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTggg                                                       >  2:832671/1‑139 (MQ=255)
                                                                          aaaGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGCTTGCCGGACTTCACATCCGTGGGTTGcc                                                  >  2:966442/1‑139 (MQ=255)
                                                                          aaaGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                        <  1:66698/117‑1 (MQ=255)
                                                                          aaaGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                        >  2:66698/1‑117 (MQ=255)
                                                                                 tGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                        >  1:465388/1‑110 (MQ=255)
                                                                                 tGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                        <  2:465388/110‑1 (MQ=255)
                                                                                 tGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGc                                           >  2:1568591/1‑139 (MQ=255)
                                                                                   ccGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCaa                                         >  2:180828/1‑139 (MQ=255)
                                                                                           cgcgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCa                                                                                                        >  2:869826/1‑68 (MQ=255)
                                                                                           cgcgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCa                                                                                                        <  1:869826/68‑1 (MQ=255)
                                                                                             cgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTg                                                    <  2:1549529/118‑1 (MQ=255)
                                                                                             cgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTg                                                    >  1:1549529/1‑118 (MQ=255)
                                                                                             cgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGa                               >  2:260007/1‑139 (MQ=255)
                                                                                                     gcgTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                        >  1:779348/1‑90 (MQ=255)
                                                                                                     gcgTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                        <  2:779348/90‑1 (MQ=255)
                                                                                                      cgTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgt                                                                                                     >  2:375562/1‑60 (MQ=255)
                                                                                                      cgTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgt                                                                                                     <  1:375562/60‑1 (MQ=255)
                                                                                                      cgTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGc                      >  2:130218/1‑139 (MQ=255)
                                                                                                              ccTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGc              >  2:1238215/1‑139 (MQ=255)
                                                                                                                          ttAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGttt  <  2:1258106/139‑1 (MQ=255)
                                                                                                                             gCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAGTGATTGCCg                                                                        >  1:1064950/1‑66 (MQ=255)
                                                                                                                             gCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                        <  2:1064950/66‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
GAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTT  >  NC_000913/1556981‑1557241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑