Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,626,825	C→T	V186I (GTT→ATT)	dcp ←	dipeptidyl carboxypeptidase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,626,825	0	C	T	100.0%	196.8 / NA	61	V186I (GTT→ATT)	dcp	dipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/29);  total (32/29)

GCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAACGT  >  NC_000913/1626695‑1626958                                                                                                                                   |                                                                                                                                      gcCGGTTGTTGGGGGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccgc                                                                                                                               <  1:988316/139‑1 (MQ=255)     gttgttGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGa                                                                                                                           >  2:838596/1‑139 (MQ=255)          tgGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGatttat                                                                                                                      >  1:1266642/1‑139 (MQ=255)               ggtATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtgct                                                                                                                 >  1:1543356/1‑139 (MQ=255)                 tATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtgctg                                                                                                                <  1:1512988/138‑1 (MQ=255)                 tATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtgctg                                                                                                                >  2:1512988/1‑138 (MQ=255)                           cGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGc                                                                                                     >  2:358429/1‑139 (MQ=255)                           cGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGc                                                                                                     >  2:1173480/1‑139 (MQ=255)                             gAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtgct                                                                                                                 >  2:500125/1‑125 (MQ=255)                             gAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtgct                                                                                                                 <  1:500125/125‑1 (MQ=255)                                  aGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTaa                                                                                              <  2:243187/139‑1 (MQ=255)                                       tttGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAActgg                                                                                         >  1:374306/1‑139 (MQ=255)                                              tCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCa                                                                                  <  1:1173480/139‑1 (MQ=255)                                              tCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCa                                                                                  >  1:502756/1‑139 (MQ=255)                                                   aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccgccg                                                                                                                             >  2:938740/1‑91 (MQ=255)                                                   aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccgccg                                                                                                                             <  1:938740/91‑1 (MQ=255)                                                   aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccg                                                                                                                                <  2:934329/88‑1 (MQ=255)                                                   aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccg                                                                                                                                >  1:934329/1‑88 (MQ=255)                                                   aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTc                                                                             >  2:1185584/1‑139 (MQ=255)                                                   aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTc                                                                             >  1:541224/1‑139 (MQ=255)                                                   aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTAGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTc                                                                             >  2:980423/1‑139 (MQ=255)                                                    ccTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCg                                                                            <  1:598841/139‑1 (MQ=255)                                                    ccTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCg                                                                            <  1:840410/139‑1 (MQ=255)                                                     cTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGc                                                                           <  2:1266642/139‑1 (MQ=255)                                                     cTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGc                                                                           <  2:1015671/139‑1 (MQ=255)                                                       ttCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATc                                                                                                       >  2:528672/1‑109 (MQ=255)                                                       ttCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATc                                                                                                       <  1:528672/109‑1 (MQ=255)                                                       ttCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                         <  2:697597/139‑1 (MQ=255)                                                       ttCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                         <  1:366963/139‑1 (MQ=255)                                                         ctcGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTA‑TTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCtt                                                                      >  2:66606/1‑139 (MQ=255)                                                            gcgAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCt                                                                    <  2:502756/139‑1 (MQ=255)                                                               aGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtg                                                                                                                   <  1:1352651/89‑1 (MQ=255)                                                               aGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtg                                                                                                                   >  2:1352651/1‑89 (MQ=255)                                                                gccgccTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTA‑TTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTAtt                                                               <  1:66606/139‑1 (MQ=255)                                                                 ccgccTCTTCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTTTTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTAtt                                                               >  1:445623/1‑139 (MQ=255)                                                                 ccgccTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTAtt                                                               >  1:1419421/1‑139 (MQ=255)                                                                 ccgccTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTAtt                                                               >  2:967914/1‑139 (MQ=255)                                                                   gccTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCa                                                             <  1:678734/139‑1 (MQ=255)                                                                          ccgccAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACttt                                                      >  2:877825/1‑139 (MQ=255)                                                                                 cgcAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGc                                                                           >  2:1135303/1‑111 (MQ=255)                                                                                 cgcAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGc                                                                           <  1:1135303/111‑1 (MQ=255)                                                                                    aaTCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCg                                                                                                      <  2:60063/81‑1 (MQ=255)                                                                                    aaTCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCg                                                                                                      >  1:60063/1‑81 (MQ=255)                                                                                           tGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAg                                     >  2:651168/1‑139 (MQ=255)                                                                                             ctcactcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCt                                   <  2:749510/139‑1 (MQ=255)                                                                                                 ctcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTgcg                               <  2:541224/139‑1 (MQ=255)                                                                                                  tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccg                                                                                                                                >  2:691863/1‑41 (MQ=255)                                                                                                  tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccg                                                                                                                                <  1:691863/41‑1 (MQ=255)                                                                                                  tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtg                                                                                                                   >  1:1251448/1‑54 (MQ=255)                                                                                                  tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtg                                                                                                                   <  2:1251448/54‑1 (MQ=255)                                                                                                  tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCg                                                                                                      >  2:519207/1‑67 (MQ=255)                                                                                                  tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCg                                                                                                      <  1:519207/67‑1 (MQ=255)                                                                                                  tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTgcgc                              >  1:591258/1‑139 (MQ=255)                                                                                                      tCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                         >  1:1361277/1‑92 (MQ=255)                                                                                                      tCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                         <  2:1361277/92‑1 (MQ=255)                                                                                                          gCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGt                                                                              <  2:758496/83‑1 (MQ=255)                                                                                                          gCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGt                                                                              >  1:758496/1‑83 (MQ=255)                                                                                                            cAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAATCTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCt                    <  1:651168/139‑1 (MQ=255)                                                                                                               cTGCTCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGc                                                                           <  1:1155397/81‑1 (MQ=255)                                                                                                               cTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGc                                                                           >  2:1155397/1‑81 (MQ=255)                                                                                                                              cacaATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAACGt  >  1:1463601/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                      GCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAACGT  >  NC_000913/1626695‑1626958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑