Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	177,662	C→T	*267* (TAG→TAA)	btuF ←	vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	177,662	0	C	T	100.0%	152.3 / NA	48	*267* (TAG→TAA)	btuF	vitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/24);  total (24/24)

GAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGCTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTA  >  NC_000913/177528‑177784                                                                                                                                       |                                                                                                                           gAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        >  2:415504/1‑139 (MQ=255)      ccAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTAcc                                                                                                                   >  1:572532/1‑139 (MQ=255)       cAAAAGGGTCCATACGCACTTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCt                                                                                                                  >  1:894865/1‑139 (MQ=255)           aGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGa                                                                                                              <  2:1040945/139‑1 (MQ=255)             ggTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGaaa                                                                                                            <  2:1299186/139‑1 (MQ=255)                ccATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAgcg                                                                                                         <  1:415504/139‑1 (MQ=255)                        aaTTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        <  1:1325431/116‑1 (MQ=255)                        aaTTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        >  2:1325431/1‑116 (MQ=255)                        aaTTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACag                                                                                                 <  1:475990/139‑1 (MQ=255)                        aaTTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACag                                                                                                 <  2:572532/139‑1 (MQ=255)                         aTTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        >  2:209303/1‑115 (MQ=255)                         aTTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        <  1:209303/115‑1 (MQ=255)                                 gCTAACAAAACGCCGGAGATGGCAAATCCCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGTGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCtgttgt                                                                                        <  2:184603/139‑1 (MQ=255)                                           cGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                               >  1:1543142/1‑122 (MQ=255)                                           cGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                               <  2:1543142/122‑1 (MQ=255)                                                          aTACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAgcg                                                                                                         >  1:1501831/1‑97 (MQ=255)                                                          aTACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAgcg                                                                                                         <  2:1501831/97‑1 (MQ=255)                                                                cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        >  2:503268/1‑76 (MQ=255)                                                                cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        <  1:503268/76‑1 (MQ=255)                                                                cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCg                                                                                  <  2:564015/114‑1 (MQ=255)                                                                cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCg                                                                                  >  1:564015/1‑114 (MQ=255)                                                                cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGTCTTGc                                                              <  1:661570/134‑1 (MQ=255)                                                                cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGc                                                              >  2:661570/1‑134 (MQ=255)                                                                cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGtt                                                         >  1:652297/1‑139 (MQ=255)                                                                         cgatatCCAACCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGt                                                <  1:952774/137‑1 (MQ=255)                                                                             atCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACt                                            <  2:652297/139‑1 (MQ=255)                                                                                  gCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACa                                                                                                  >  2:777009/1‑80 (MQ=255)                                                                                  gCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACa                                                                                                  <  1:777009/80‑1 (MQ=255)                                                                                      aTAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAgg                                   >  1:596593/1‑139 (MQ=255)                                                                                                    ttCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCg                                                                                  <  1:188039/78‑1 (MQ=255)                                                                                                    ttCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCg                                                                                  >  2:188039/1‑78 (MQ=255)                                                                                                    ttCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGt                                         >  1:540022/1‑119 (MQ=255)                                                                                                    ttCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGt                                         <  2:540022/119‑1 (MQ=255)                                                                                                    ttCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAAttt                     >  2:1101792/1‑139 (MQ=255)                                                                                                         tCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAg                 <  1:450688/138‑1 (MQ=255)                                                                                                         tCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAg                 >  2:450688/1‑138 (MQ=255)                                                                                                         tCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGc                >  2:51136/1‑139 (MQ=255)                                                                                                         tCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGc                >  1:675950/1‑139 (MQ=255)                                                                                                             cACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGtt            <  2:894865/139‑1 (MQ=255)                                                                                                              aCTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTc           >  1:597886/1‑139 (MQ=255)                                                                                                                  aGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTc                                           <  2:426473/103‑1 (MQ=255)                                                                                                                  aGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTc                                           >  1:426473/1‑103 (MQ=255)                                                                                                                   gCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCAcccc      >  2:30083/1‑139 (MQ=255)                                                                                                                       cTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTa  <  1:1101792/139‑1 (MQ=255)                                                                                                                                 cAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCa                                                             >  2:308530/1‑70 (MQ=255)                                                                                                                                 cAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCa                                                             <  1:308530/70‑1 (MQ=255)                                                                                                                                   ggCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAg                                                                                    >  1:839219/1‑45 (MQ=255)                                                                                                                                   ggCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAg                                                                                    <  2:839219/45‑1 (MQ=255)                                                                                                                                       |                                                                                                                           GAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGCTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTA  >  NC_000913/177528‑177784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑