Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,788,278 G→A *278* (TAG→TAA ppsR → PEP synthase kinase and PEP synthase pyrophosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,788,2780GA100.0% 138.2 / NA 44*278* (TAG→TAAppsRPEP synthase kinase and PEP synthase pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/17);  total (27/17)

GGATGGAAGTCGCGGAAGTGGAAGCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGC  >  NC_000913/1788145‑1788404
                                                                                                                                     |                                                                                                                              
ggATGGAAGTCGCGGAAGTGGAAGCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAAc                                                                                                                           <  1:136078/139‑1 (MQ=255)
                         cTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGtt                                                                                                  >  1:749216/1‑139 (MQ=255)
                          ttGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTa                                                                                                 <  1:1378378/139‑1 (MQ=255)
                            gTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATc                                                                                               >  1:143325/1‑139 (MQ=255)
                                  tAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTa                                                                                         >  2:14072/1‑139 (MQ=255)
                                   aaaaaTCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTaaag                                                                                                                              >  2:338938/1‑101 (MQ=255)
                                     aaaTCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAacc                                                                                      <  1:971534/139‑1 (MQ=255)
                                          aGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCg                                                                                 >  1:329139/1‑139 (MQ=255)
                                                         aCAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCa                                                                                                                    >  1:1159732/1‑89 (MQ=255)
                                                         aCAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCa                                                                                                                    <  2:1159732/89‑1 (MQ=255)
                                                            gTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCt                                                               <  1:14072/139‑1 (MQ=255)
                                                              aCCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCg                                                             >  1:1355021/1‑139 (MQ=255)
                                                               ccAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGc                                                            >  2:1278641/1‑139 (MQ=255)
                                                               ccAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGc                                                            >  1:446617/1‑139 (MQ=255)
                                                                 aaTTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCAc                                                          <  2:749216/139‑1 (MQ=255)
                                                                  attattCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCAc                                                          >  1:14798/1‑138 (MQ=255)
                                                                  attattCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCAc                                                          <  2:14798/138‑1 (MQ=255)
                                                                     attCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGtt                                                                                                  <  1:448877/95‑1 (MQ=255)
                                                                     attCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGtt                                                                                                  >  2:448877/1‑95 (MQ=255)
                                                                             gaagaGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATcagca                                              >  1:8809/1‑139 (MQ=255)
                                                                              aagaGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATcagcag                                             >  1:528581/1‑139 (MQ=255)
                                                                                  gaTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGAt                                         <  1:1278641/139‑1 (MQ=255)
                                                                                     tGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTgg                                      >  1:976746/1‑139 (MQ=255)
                                                                                     tGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTgg                                      >  2:1349115/1‑139 (MQ=255)
                                                                                            aaGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCg                               >  1:1573622/1‑139 (MQ=255)
                                                                                             aGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGc                                                                                                               <  1:764339/58‑1 (MQ=255)
                                                                                             aGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGc                                                                                                               >  2:764339/1‑58 (MQ=255)
                                                                                                  cTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCt                                                               >  2:1155086/1‑101 (MQ=255)
                                                                                                  cTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCt                                                               <  1:1155086/101‑1 (MQ=255)
                                                                                                    cGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATgcgc                       >  1:1316194/1‑139 (MQ=255)
                                                                                                      atatCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCAt                     <  2:329139/139‑1 (MQ=255)
                                                                                                         tCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCAc                  >  2:1488010/1‑139 (MQ=255)
                                                                                                            tGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCAt                                                                                                                   <  1:482943/39‑1 (MQ=255)
                                                                                                            tGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCAt                                                                                                                   >  2:482943/1‑39 (MQ=255)
                                                                                                            tGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTc               >  1:1221166/1‑139 (MQ=255)
                                                                                                               gCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACGCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCg            >  1:939949/1‑139 (MQ=255)
                                                                                                                 cTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGc          >  2:191364/1‑139 (MQ=255)
                                                                                                                    aGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAg                                                                            <  1:504223/70‑1 (MQ=255)
                                                                                                                    aGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAg                                                                            >  2:504223/1‑70 (MQ=255)
                                                                                                                    aGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGacaca                                                                  <  2:405432/80‑1 (MQ=255)
                                                                                                                    aGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGacaca                                                                  >  1:405432/1‑80 (MQ=255)
                                                                                                                     gTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTc      <  2:143325/139‑1 (MQ=255)
                                                                                                                         ccGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGc  >  2:1123958/1‑139 (MQ=255)
                                                                                                                          cGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCa                                                                                              >  2:1599300/1‑46 (MQ=255)
                                                                                                                          cGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCa                                                                                              <  1:1599300/46‑1 (MQ=255)
                                                                                                                                     |                                                                                                                              
GGATGGAAGTCGCGGAAGTGGAAGCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGC  >  NC_000913/1788145‑1788404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: