Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,826,546	T→C	E126E (GAA→GAG)	astE ←	succinylglutamate desuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,826,546	0	T	C	100.0%	248.0 / NA	68	E126E (GAA→GAG)	astE	succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/31);  total (37/31)

GTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAG  >  NC_000913/1826429‑1826671                                                                                                                      |                                                                                                                              gTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAg                                                                                                          >  2:604138/1‑139 (MQ=255)     cgTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGttt                                                                                                      >  1:358819/1‑139 (MQ=255)      gTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGCCAGGTTtc                                                                                                     >  1:983815/1‑139 (MQ=255)                    ccGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCaa                                                                                       >  2:1115717/1‑139 (MQ=255)                     cGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCaaa                                                                                      >  1:804665/1‑139 (MQ=255)                       aaCTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATa                                                                                    >  1:1467337/1‑139 (MQ=255)                       aaCTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATa                                                                                    >  1:1504352/1‑139 (MQ=255)                                   aaGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGAc                                                                        <  2:358819/139‑1 (MQ=255)                                                 tGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGa                                                          <  1:604138/139‑1 (MQ=255)                                                       aTGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACAtt                                                             >  1:916094/1‑130 (MQ=255)                                                       aTGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACAtt                                                             <  2:916094/130‑1 (MQ=255)                                                        tGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAgg                                                                                                         <  1:1249292/85‑1 (MQ=255)                                                        tGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAgg                                                                                                         >  2:1249292/1‑85 (MQ=255)                                                        tGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCtt                                                                                              <  1:1391383/96‑1 (MQ=255)                                                        tGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCtt                                                                                              >  2:1391383/1‑96 (MQ=255)                                                        tGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAAc                                                                           <  2:349521/115‑1 (MQ=255)                                                        tGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAAc                                                                           >  1:349521/1‑115 (MQ=255)                                                        tGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCatat                                                   >  1:676548/1‑139 (MQ=255)                                                            gATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCt                                               <  2:1467337/139‑1 (MQ=255)                                                             aTCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACAtt                                                             <  2:797219/124‑1 (MQ=255)                                                             aTCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACAtt                                                             >  1:797219/1‑124 (MQ=255)                                                             aTCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTa                                              <  1:1115717/139‑1 (MQ=255)                                                                aaGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATgg                                           >  2:540926/1‑139 (MQ=255)                                                                 aGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGc                                          <  2:1504352/139‑1 (MQ=255)                                                                  ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCTGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                         >  2:1054771/1‑139 (MQ=255)                                                                  ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGc                                                                                  <  2:815705/98‑1 (MQ=255)                                                                  ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGc                                                                                  >  1:815705/1‑98 (MQ=255)                                                                  ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACAtt                                                             <  2:1101077/119‑1 (MQ=255)                                                                  ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACAtt                                                             >  1:1101077/1‑119 (MQ=255)                                                                  ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                         >  2:968934/1‑139 (MQ=255)                                                                  ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                         >  1:816252/1‑139 (MQ=255)                                                                  ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                         >  1:1204721/1‑139 (MQ=255)                                                                    tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAAt                                       <  2:1584317/139‑1 (MQ=255)                                                                       cAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAAc                                    >  1:1318460/1‑139 (MQ=255)                                                                       cAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAAc                                    >  2:1586304/1‑139 (MQ=255)                                                                            cacaGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTa                                              <  2:1235502/124‑1 (MQ=255)                                                                            cacaGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTa                                              >  1:1235502/1‑124 (MQ=255)                                                                                       tGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGAcc                                                                       <  2:360026/88‑1 (MQ=255)                                                                                       tGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGAcc                                                                       >  1:360026/1‑88 (MQ=255)                                                                                       tGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGt                                  >  2:1236865/1‑125 (MQ=255)                                                                                       tGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGt                                  <  1:1236865/125‑1 (MQ=255)                                                                                       tGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCtt                       <  2:578361/136‑1 (MQ=255)                                                                                       tGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCtt                       >  1:578361/1‑136 (MQ=255)                                                                                         cccTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAgcg                  <  2:1204721/139‑1 (MQ=255)                                                                                         cccTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAgcg                  <  1:1576864/139‑1 (MQ=255)                                                                                           cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcg                                                                                                                    <  1:1424015/39‑1 (MQ=255)                                                                                           cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcg                                                                                                                    >  2:1424015/1‑39 (MQ=38)                                                                                           cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAg                                                                                   >  1:586454/1‑72 (MQ=255)                                                                                           cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAg                                                                                   <  2:586454/72‑1 (MQ=255)                                                                                           cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCa                >  2:647950/1‑139 (MQ=255)                                                                                             ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGa                                                          <  1:599081/95‑1 (MQ=255)                                                                                             ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGa                                                          >  2:599081/1‑95 (MQ=255)                                                                                             ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTa                                              >  1:51046/1‑107 (MQ=255)                                                                                             ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTa                                              <  2:51046/107‑1 (MQ=255)                                                                                             ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCagg              <  2:886659/139‑1 (MQ=255)                                                                                              gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGc                                                <  1:544591/104‑1 (MQ=255)                                                                                              gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGc                                                >  2:544591/1‑104 (MQ=255)                                                                                              gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCagga             >  2:217133/1‑139 (MQ=255)                                                                                              gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCagga             >  2:802822/1‑139 (MQ=255)                                                                                              gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCag               >  1:175231/1‑137 (MQ=255)                                                                                              gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCag               <  2:175231/137‑1 (MQ=255)                                                                                                cATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCaggagg           <  1:647950/139‑1 (MQ=255)                                                                                                 aTAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAggagga          <  2:816252/139‑1 (MQ=255)                                                                                                 aTAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAggagga          <  2:1318460/139‑1 (MQ=255)                                                                                                        tCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGa   >  2:129577/1‑139 (MQ=255)                                                                                                        tCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGACTCCCGa   >  2:440156/1‑139 (MQ=255)                                                                                                         cTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTCTCCCTTGCTTCAGCGCAGGAGGATTCCCGAg  <  1:968934/139‑1 (MQ=255)                                                                                                                   cTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACAt                                                              <  2:26874/69‑1 (MQ=255)                                                                                                                      |                                                                                                                              GTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAG  >  NC_000913/1826429‑1826671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑