Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,022,606	G→A	*246* (TAG→TAA)	fliP →	flagellar biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,022,606	0	G	A	100.0%	192.6 / NA	59	*246* (TAG→TAA)	fliP	flagellar biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/29);  total (30/29)

CCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCAC  >  NC_000913/2022470‑2022740                                                                                                                                         |                                                                                                                                       ccAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTaaag                                                                                                                                      >  2:915910/1‑139 (MQ=255)   aGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTaaagag                                                                                                                                    >  2:1460407/1‑139 (MQ=255)    gCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAgg                                                                                                                                   >  1:101097/1‑139 (MQ=255)    gCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAgg                                                                                                                                   <  1:467517/139‑1 (MQ=255)             tGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAgg                                                                                                                                   <  2:533912/130‑1 (MQ=255)             tGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAgg                                                                                                                                   >  1:533912/1‑130 (MQ=255)                    ggggATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGaa                                                                                                                   <  2:1226170/139‑1 (MQ=255)                                 gTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatgatga                                                                                                      >  1:837818/1‑139 (MQ=255)                                       ccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                  >  1:807484/1‑105 (MQ=255)                                       ccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                  <  2:807484/105‑1 (MQ=255)                                            caccaTTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGaa                                                                                           <  2:101097/139‑1 (MQ=255)                                                aTTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGa                                                                                       >  1:1165960/1‑139 (MQ=255)                                                aTTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGa                                                                                       >  2:717781/1‑139 (MQ=255)                                                    ctctGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatgatgat                                                                                                     >  1:1184591/1‑121 (MQ=255)                                                    ctctGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatgatgat                                                                                                     <  2:1184591/121‑1 (MQ=255)                                                    ctctGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGaa                                                                                   >  1:877334/1‑139 (MQ=255)                                                     tctGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGaaa                                                                                  >  1:671837/1‑139 (MQ=255)                                                           cTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                            <  2:292737/139‑1 (MQ=255)                                                           cTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                            <  2:837818/139‑1 (MQ=255)                                                            tttAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCgcgc                                                                           >  1:592073/1‑139 (MQ=255)                                                                   tGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACt                                                                    >  1:702036/1‑139 (MQ=255)                                                                    gATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTg                                                                   >  2:1312819/1‑139 (MQ=255)                                                                        cTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTg                                                               <  1:1252482/139‑1 (MQ=255)                                                                           tttGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGccc                                                            <  1:1460407/139‑1 (MQ=255)                                                                                aCTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCg                                                                                                                <  2:1235091/82‑1 (MQ=255)                                                                                aCTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCg                                                                                                                >  1:1235091/1‑82 (MQ=255)                                                                                    gtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAAt                                                                                                                  >  2:102147/1‑76 (MQ=255)                                                                                    gtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAAt                                                                                                                  <  1:102147/76‑1 (MQ=255)                                                                                     tggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                            <  1:1297099/113‑1 (MQ=255)                                                                                     tggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                            >  2:1297099/1‑113 (MQ=255)                                                                                     tggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTAttgtt                                                  >  1:1225767/1‑139 (MQ=255)                                                                                     tggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTAttgtt                                                  >  1:340653/1‑139 (MQ=255)                                                                                       gATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTgg                                                <  2:1165960/139‑1 (MQ=255)                                                                                         tggctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAAt                                                                                                                             >  1:872521/1‑60 (MQ=255)                                                                                         tggctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAAt                                                                                                                             <  2:872521/60‑1 (MQ=255)                                                                                         tggctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatg                                                                                                          >  1:567096/1‑79 (MQ=255)                                                                                         tggctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatg                                                                                                          <  2:567096/79‑1 (MQ=255)                                                                                         tggctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGc                                                              >  1:749259/1‑123 (MQ=255)                                                                                         tggctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGc                                                              <  2:749259/123‑1 (MQ=255)                                                                                            ctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTAtt                                                     >  1:1515529/1‑129 (MQ=255)                                                                                            ctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTAtt                                                     <  2:1515529/129‑1 (MQ=255)                                                                                              ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGa                                                                                                                    >  1:877486/1‑64 (MQ=255)                                                                                              ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGa                                                                                                                    <  2:877486/64‑1 (MQ=255)                                                                                              ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                            >  1:1075203/1‑104 (MQ=255)                                                                                              ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                            <  2:1075203/104‑1 (MQ=255)                                                                                              ggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGtt                                         <  2:592073/139‑1 (MQ=255)                                                                                               gcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTg                                        <  2:1225767/139‑1 (MQ=255)                                                                                               gcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTg                                        >  2:1435323/1‑139 (MQ=255)                                                                                                    tGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCAc                                   <  1:717781/139‑1 (MQ=255)                                                                                                     gCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACt                                                                    <  2:171875/105‑1 (MQ=255)                                                                                                     gCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACt                                                                    >  1:171875/1‑105 (MQ=255)                                                                                                      cTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACgg                                 >  2:600353/1‑139 (MQ=255)                                                                                                         gTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGcc                              >  2:163572/1‑139 (MQ=255)                                                                                                             gTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTat                          >  1:516037/1‑139 (MQ=255)                                                                                                                cGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTatcat                       <  2:702036/139‑1 (MQ=255)                                                                                                                cGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTatcat                       <  2:378226/139‑1 (MQ=255)                                                                                                                cGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTatcat                       <  1:1562304/139‑1 (MQ=255)                                                                                                                cGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTatcat                       <  1:915910/139‑1 (MQ=255)                                                                                                                  cTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTAtcatca                     <  1:1514907/139‑1 (MQ=255)                                                                                                                            gCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCa           >  1:1394163/1‑139 (MQ=255)                                                                                                                                     gCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCAc  >  2:1554001/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       CCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCAC  >  NC_000913/2022470‑2022740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑