Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	198,075	A→G	T50A (ACA→GCA)	bamA →	BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	198,075	0	A	G	100.0%	272.4 / NA	76	T50A (ACA→GCA)	bamA	BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/44);  total (32/44)

AGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGATGGTGATACCCTTCTGGTTCAGGTAAAAGAACGTCCGACCA  >  NC_000913/197941‑198207                                                                                                                                       |                                                                                                                                     aGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAgg                                                                                                                                  >  2:918001/1‑139 (MQ=255)     gCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGac                                                                                                                              <  2:1315013/139‑1 (MQ=255)      cTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGaca                                                                                                                             <  1:951200/139‑1 (MQ=255)      cTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGaca                                                                                                                             >  2:138048/1‑139 (MQ=255)          tAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGt                                                                                                                         <  1:1082394/139‑1 (MQ=255)               tCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAatg                                                                                                                    <  2:36295/139‑1 (MQ=255)                 gctgctGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAatgat                                                                                                                  <  2:925835/139‑1 (MQ=255)                 gctgctGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAatgat                                                                                                                  <  1:1228986/139‑1 (MQ=255)                  ctgctgTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAatgatg                                                                                                                 <  2:474148/139‑1 (MQ=255)                  ctgctgTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCGCAGTATGCCGGTGCGCGCAGGCGACACGGTTAatgatg                                                                                                                 <  1:1425078/139‑1 (MQ=255)                   tgctgTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGGCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAAtgatga                                                                                                                <  1:1132451/139‑1 (MQ=255)                     ctgTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTGGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAg                                                                                                              >  1:1022700/1‑139 (MQ=255)                     ctgTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAg                                                                                                              <  1:358915/139‑1 (MQ=255)                       gTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGat                                                                                                            <  2:173389/139‑1 (MQ=255)                        tttAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGata                                                                                                           <  1:1080174/139‑1 (MQ=255)                        tttAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGata                                                                                                           <  1:918001/139‑1 (MQ=255)                         ttAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGatat                                                                                                          <  1:138048/139‑1 (MQ=255)                               gcgcCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGac                                                                                                                              <  2:403374/113‑1 (MQ=255)                               gcgcCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGac                                                                                                                              >  1:403374/1‑113 (MQ=255)                               gcgcCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTaa                                                                                                    >  2:1376862/1‑139 (MQ=255)                                       gTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGa                                                                                                                               >  2:754830/1‑104 (MQ=255)                                       gTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGa                                                                                                                               <  1:754830/104‑1 (MQ=255)                                         ataCGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTcgc                                                                                          <  1:907324/139‑1 (MQ=255)                                                gCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGt                                                                                   >  2:1506628/1‑139 (MQ=255)                                                gCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGt                                                                                   >  2:164405/1‑139 (MQ=255)                                                 cTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGtt                                                                                  >  1:1527015/1‑139 (MQ=255)                                                  tGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGac                                                                                                                              <  1:1392710/94‑1 (MQ=255)                                                  tGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGac                                                                                                                              >  2:1392710/1‑94 (MQ=255)                                                  tGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAatgatg                                                                                                                 <  2:583303/107‑1 (MQ=255)                                                  tGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAatgatg                                                                                                                 >  1:583303/1‑107 (MQ=255)                                                         ttCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGac                                                                                                                              <  2:1234858/87‑1 (MQ=255)                                                         ttCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGac                                                                                                                              >  1:1234858/1‑87 (MQ=255)                                                         ttCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGat                                                                                                            >  2:644214/1‑105 (MQ=255)                                                         ttCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGat                                                                                                            <  1:644214/105‑1 (MQ=255)                                                           cGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGc                                                                        >  2:789639/1‑139 (MQ=255)                                                           cGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGc                                                                        <  1:1610085/139‑1 (MQ=255)                                                           cGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGc                                                                        >  2:728332/1‑139 (MQ=255)                                                                   aaGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGa                                                                >  1:78292/1‑139 (MQ=255)                                                                      atatTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCGCTCCTCAGTATGCCGGTGCGCGCAGGCGACATGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGa                                                             <  1:1047718/139‑1 (MQ=255)                                                                      atatTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCTCTCTGTTTGCTACCGGCAACTTTGAGGa                                                             <  2:997855/139‑1 (MQ=255)                                                                        atTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGata                                                                                                           >  1:18928/1‑91 (MQ=255)                                                                        atTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGata                                                                                                           <  2:18928/91‑1 (MQ=255)                                                                          tCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGtt                                                         >  2:270509/1‑139 (MQ=255)                                                                          tCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGtt                                                         <  1:789639/139‑1 (MQ=255)                                                                               tCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGat                                                                                                            >  2:1103689/1‑83 (MQ=255)                                                                               tCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGat                                                                                                            <  1:1103689/83‑1 (MQ=255)                                                                               tCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTcgcg                                                                                         <  1:155698/102‑1 (MQ=255)                                                                               tCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTcgcg                                                                                         >  2:155698/1‑102 (MQ=255)                                                                                 gAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGaca                                                                                                                             <  1:725883/64‑1 (MQ=255)                                                                                 gAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGaca                                                                                                                             >  2:725883/1‑64 (MQ=255)                                                                                 gAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTcc                                                  >  2:1111860/1‑139 (MQ=255)                                                                                   aGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAgg                                                              >  2:928880/1‑125 (MQ=255)                                                                                   aGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAgg                                                              <  1:928880/125‑1 (MQ=255)                                                                                      ccTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGt                                             <  1:1506628/139‑1 (MQ=255)                                                                                       cTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTg                                            <  2:58702/139‑1 (MQ=255)                                                                                        ttCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCATTCGACACGGTTAATGATGAAGATACCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGa                                           <  2:1022700/139‑1 (MQ=255)                                                                                        ttCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTcgc                                                      >  2:159771/1‑128 (MQ=255)                                                                                        ttCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTcgc                                                      <  1:159771/128‑1 (MQ=255)                                                                                        ttCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGa                                           <  1:1376862/139‑1 (MQ=255)                                                                                                 tCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTcgcg                                                     <  2:1517667/120‑1 (MQ=255)                                                                                                 tCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTcgcg                                                     >  1:1517667/1‑120 (MQ=255)                                                                                                 gcGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGATGGTGATAc                                  >  1:1006199/2‑139 (MQ=255)                                                                                                     cGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGATGGTGATACCCtt                              <  1:1111860/139‑1 (MQ=255)                                                                                                      gTTGGTGCGGACCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGCGCTTATTCGTAATACCCTTCGCGCTCTGTTTGCTCCCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGATGGTGATACCCtgc                             <  1:1006004/139‑3 (MQ=255)                                                                                                            gCGGCCCTCCTCAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGATGTTGATACCCTTCTGGTTc                       <  2:1006199/139‑1 (MQ=255)                                                                                                                  ctcctcAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTcgcg                                                                                         <  2:496075/67‑1 (MQ=255)                                                                                                                  ctcctcAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTcgcg                                                                                         >  1:496075/1‑67 (MQ=255)                                                                                                                    cctcAGTATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGATGGTGATACCCTTCTGGTTCAGGTaaaa               >  2:381394/1‑139 (MQ=255)                                                                                                                          tATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCt                                                 <  2:619284/99‑1 (MQ=255)                                                                                                                          tATGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCt                                                 >  1:619284/1‑99 (MQ=255)                                                                                                                           aTGCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGATGGTGATACCCTTCTGGTTCAGGTAAAAGAACGTc        <  2:78292/139‑1 (MQ=255)                                                                                                                             gCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGat                                                                                                            >  2:169269/1‑37 (MQ=255)                                                                                                                             gCCGGTGCGCGCAGGCGACACGGTTAATGATGAAGat                                                                                                            <  1:169269/37‑1 (MQ=255)                                                                                                                               cGGTGCGCGCAGGCGACACGGTTAATGATGAAGat                                                                                                            >  2:1574833/1‑35 (MQ=255)                                                                                                                               cGGTGCGCGCAGGCGACACGGTTAATGATGAAGat                                                                                                            <  1:1574833/35‑1 (MQ=255)                                                                                                                                 gTGCGCGCAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGATGGTGATACCCTTCTGGTTCAGGTAAAAGAACGTCCGACCa  >  2:391216/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                     AGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGATGGTGATACCCTTCTGGTTCAGGTAAAAGAACGTCCGACCA  >  NC_000913/197941‑198207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑