Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,085,831 A→G L411P (CTG→CCG)  plaP ← putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,8310AG100.0% 946.7 / NA 261L411P (CTG→CCG) plaPputrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (134/127);  total (134/127)

Only 100 of 261 total aligned reads displayed.

AAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAG  >  NC_000913/2085696‑2085964
                                                                                                                                       |                                                                                                                                     
aaaGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                    >  2:110873/1‑139 (MQ=255)
  aGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCa                                                                                                                                  >  2:1534191/1‑139 (MQ=255)
      gatTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCaa                                                                                                                              >  2:946305/1‑139 (MQ=255)
      gatTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCaa                                                                                                                              >  2:116075/1‑139 (MQ=255)
          aTGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                          <  2:1369609/139‑1 (MQ=255)
              tACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgc                                                                                                                      <  1:368973/139‑1 (MQ=255)
               aCGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                     <  1:307676/139‑1 (MQ=255)
                cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCa                                                                                                                               >  1:1005332/1‑128 (MQ=255)
                cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCa                                                                                                                               <  2:1005332/128‑1 (MQ=255)
                cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcgc                                                                                                                    >  2:1330121/1‑139 (MQ=255)
                cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcgc                                                                                                                    >  1:422974/1‑139 (MQ=255)
                  tCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                                  >  2:1481698/1‑139 (MQ=255)
                  tCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                                  >  1:390179/1‑139 (MQ=255)
                     tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcaccac                                                                                                               >  2:274520/1‑139 (MQ=255)
                     tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcaccac                                                                                                               >  1:812507/1‑139 (MQ=255)
                      cGTACTGCGGAACCGGCTTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCCCGGCGCACCAACGGTCAGCGCACcaca                                                                                                              >  1:202443/1‑139 (MQ=255)
                      cGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                    <  2:957651/117‑1 (MQ=255)
                      cGTACTGCGGAACCGGATTACGGAAGCTCTTGCTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                    >  1:957651/1‑117 (MQ=255)
                        tACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  1:1256911/139‑1 (MQ=255)
                        tACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  1:769500/139‑1 (MQ=255)
                            gCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                        >  2:1238105/1‑139 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCATCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  1:1564451/1‑139 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGca                                                                                                                   <  1:1597170/127‑1 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  1:112545/1‑139 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       <  1:787498/139‑1 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  1:1469150/1‑139 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  1:510124/1‑139 (MQ=255)
                                aaCCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAgg                                                                                                    <  1:1238105/139‑1 (MQ=255)
                                  ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  <  1:766717/139‑1 (MQ=255)
                                          cGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                      >  2:450453/1‑127 (MQ=255)
                                          cGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACt                                                                                          >  1:1196780/1‑139 (MQ=255)
                                          cGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACt                                                                                          >  1:564603/1‑139 (MQ=255)
                                           ggAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTg                                                                                         <  1:1542717/139‑1 (MQ=255)
                                               gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAg                                                                                     >  1:69780/1‑139 (MQ=255)
                                                ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                     >  1:439127/1‑106 (MQ=255)
                                                ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAg                                                                                                                       <  1:126986/104‑1 (MQ=255)
                                                          cGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAg                                                                          >  1:257132/1‑139 (MQ=255)
                                                           gCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAAc                                                                                                 >  1:903070/1‑115 (MQ=255)
                                                            cAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                        <  1:98674/139‑1 (MQ=255)
                                                             aaGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGa                                                                                                   >  2:1308343/1‑111 (MQ=255)
                                                             aaGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGt                                                                       <  2:899211/139‑1 (MQ=255)
                                                               gCCAGGTAACTCAGACCGATAGCAGCCCAGATCATACCCAGAACCATTGAGCTTGCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                          <  1:1152537/86‑1 (MQ=255)
                                                               gCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                          >  2:1152537/1‑86 (MQ=255)
                                                               gCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCt                                                                     <  2:173778/139‑1 (MQ=255)
                                                                    gTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                          >  1:267369/1‑81 (MQ=255)
                                                                    gTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTa                                                                >  2:1516704/1‑139 (MQ=255)
                                                                      aaaTCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              <  2:514866/139‑1 (MQ=255)
                                                                      aaaTCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              <  1:1604735/139‑1 (MQ=255)
                                                                      aaaTCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              <  2:571548/139‑1 (MQ=255)
                                                                         tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  <  2:48642/100‑1 (MQ=255)
                                                                         tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  <  2:1259639/100‑1 (MQ=255)
                                                                             aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCa                                                       >  2:1291349/1‑139 (MQ=255)
                                                                              ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      <  1:149796/139‑1 (MQ=255)
                                                                                  tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCa                                                                           <  1:1517966/114‑1 (MQ=255)
                                                                                   agcagcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGc                                                             <  2:416291/127‑1 (MQ=255)
                                                                                     cagcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  >  1:741122/1‑88 (MQ=255)
                                                                                       gcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      >  2:72168/1‑130 (MQ=255)
                                                                                       gcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      <  1:72168/130‑1 (MQ=255)
                                                                                         ccAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAAc                                           <  1:683648/139‑1 (MQ=255)
                                                                                           aGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  1:962611/1‑77 (MQ=255)
                                                                                           aGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       <  2:962611/77‑1 (MQ=255)
                                                                                             aTCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCg                                       >  1:520913/1‑139 (MQ=255)
                                                                                                aGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCgaga                                    <  2:1559396/139‑1 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                    >  1:833677/1‑41 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                     >  2:664243/1‑56 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  >  1:1330552/1‑75 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAg                                                                                     >  2:1414998/1‑88 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAg                                                                                     <  1:1414998/88‑1 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATg                                  >  2:337042/1‑139 (MQ=255)
                                                                                                      aGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                        <  2:759280/97‑1 (MQ=255)
                                                                                                      aGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                              >  1:1326027/1‑139 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                >  2:215875/1‑72 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCt                                                            >  1:1296552/1‑108 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      <  2:1597276/114‑1 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                             <  2:1564451/139‑1 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                             <  2:822329/139‑1 (MQ=255)
                                                                                                        aaCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                            <  1:1001046/139‑1 (MQ=255)
                                                                                                            aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                                  <  2:558782/49‑1 (MQ=255)
                                                                                                             ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  1:1551192/1‑59 (MQ=255)
                                                                                                             ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                            >  2:246446/1‑134 (MQ=255)
                                                                                                             ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGtt                       >  2:295441/1‑139 (MQ=255)
                                                                                                              tGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCg                                       <  1:1380502/122‑1 (MQ=255)
                                                                                                                  cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGa                                                                                              <  1:72775/63‑1 (MQ=255)
                                                                                                                  cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGa                                                                                              >  2:72775/1‑63 (MQ=255)
                                                                                                                  cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              >  2:871554/1‑95 (MQ=255)
                                                                                                                  cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              <  1:871554/95‑1 (MQ=255)
                                                                                                                    ttcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  <  2:1063943/57‑1 (MQ=255)
                                                                                                                     tcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAg               >  2:1208376/1‑139 (MQ=255)
                                                                                                                     tcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCCCACGGATCCAGAACTGCGAGAt                                   <  2:797183/119‑1 (MQ=255)
                                                                                                                            ggTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  1:1096674/1‑44 (MQ=255)
                                                                                                                            ggTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGaaa                <  2:786362/131‑1 (MQ=255)
                                                                                                                            ggTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGc        >  2:69121/1‑139 (MQ=255)
                                                                                                                               tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTtct                                                         <  2:602535/87‑1 (MQ=255)
                                                                                                                               tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                            <  1:485390/116‑1 (MQ=255)
                                                                                                                                aaCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              >  1:616598/1‑81 (MQ=255)
                                                                                                                                 aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCtt                                                           >  1:170690/1‑83 (MQ=255)
                                                                                                                                 aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      <  1:587555/88‑1 (MQ=255)
                                                                                                                                 aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccacca   <  2:520913/139‑1 (MQ=255)
                                                                                                                                 aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccacca   >  2:170393/1‑139 (MQ=255)
                                                                                                                                  cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAg  <  1:240643/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
AAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAG  >  NC_000913/2085696‑2085964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: