Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,130,853	C→T	S9N (AGC→AAC)  *406* (TAG→TAA)	wcaD ← wcaC ←	putative colanic acid polymerase putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,130,853	0	C	T	100.0%	184.9 / NA	57	S9N (AGC→AAC)  *406* (TAG→TAA)	wcaD wcaC	putative colanic acid polymerase putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

ATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCA  >  NC_000913/2130730‑2130986                                                                                                                            |                                                                                                                                      aTGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTa                                                                                                                                     >  2:659490/1‑126 (MQ=255) aTGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTa                                                                                                                                     <  1:659490/126‑1 (MQ=255)  tGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                                       <  1:1081161/139‑1 (MQ=255)  tGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                                       <  2:1259894/139‑1 (MQ=255)           aaaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACAt                                                                                                              >  1:178104/1‑139 (MQ=255)                agcagTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActcc                                                                                                         >  1:542417/1‑139 (MQ=255)                 gcagTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTGACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActcct                                                                                                        >  1:1085160/1‑139 (MQ=255)                   agTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAt                                                                                                                                <  2:80592/113‑1 (MQ=255)                   agTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAt                                                                                                                                >  1:80592/1‑113 (MQ=255)                      aaCAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGc                                                                                                   <  2:231736/139‑1 (MQ=255)                        cAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCAt                                                                                                 <  2:496269/139‑1 (MQ=255)                          aGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                                       >  2:860932/1‑115 (MQ=255)                          aGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                                       <  1:860932/115‑1 (MQ=255)                          aGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCt                                                                                               <  2:85353/139‑1 (MQ=255)                           gACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtg                                                                                              <  2:114263/139‑1 (MQ=255)                           gACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtg                                                                                              <  2:1570457/139‑1 (MQ=255)                               ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAt                                                                                                                                <  1:676917/101‑1 (MQ=255)                               ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAt                                                                                                                                >  2:676917/1‑101 (MQ=255)                               ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTc                                                                                                                              <  2:1499769/103‑1 (MQ=255)                               ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTc                                                                                                                              >  1:1499769/1‑103 (MQ=255)                               ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                          <  2:271863/139‑1 (MQ=255)                               ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                          >  1:66799/1‑139 (MQ=255)                               ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                          >  2:440831/1‑139 (MQ=255)                               ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                          <  2:66799/139‑1 (MQ=255)                                ggaaaaaaGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                          <  1:1071393/136‑1 (MQ=255)                                gTAAAAAAGTGACGATTGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAt                                                                                                                                <  2:855325/100‑1 (MQ=255)                                gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAt                                                                                                                                >  1:855325/1‑100 (MQ=255)                                gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                          >  2:1071393/1‑138 (MQ=255)                                 tAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTcc                                                                                        <  1:440831/139‑1 (MQ=255)                                                       gggAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGt                                                                  <  2:410462/139‑1 (MQ=255)                                                           aaCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGt                                                                  <  2:492309/135‑1 (MQ=255)                                                           aaCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGt                                                                  >  1:492309/1‑135 (MQ=255)                                                           aaCTGTCGCCAAGGTGGGCGATTGTGACGTTAACCAGCAGATAAATCGGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCGGTGTAGGCGGCGCGGCTTAGTTGGc                                                              >  1:215448/1‑139 (MQ=255)                                                            aCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCt                                                             >  2:1591447/1‑139 (MQ=255)                                                            aCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCt                                                             >  2:1608909/1‑139 (MQ=255)                                                              tttCGCCAAGCTGGGCGATTTTGACTTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCAACTGTAGGCGGCGCGGCTGCGTTGGCTGa                                                           >  1:1127141/1‑139 (MQ=255)                                                               ttCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGaa                                                          <  2:906329/139‑1 (MQ=255)                                                               ttCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGaa                                                          <  1:1591447/139‑1 (MQ=255)                                                                       gCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTcagcca                                                  >  1:559459/1‑139 (MQ=255)                                                                        cTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                          >  1:270522/1‑98 (MQ=255)                                                                        cTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                          <  2:270522/98‑1 (MQ=255)                                                                        cTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTcagccag                                                 >  2:1582426/1‑139 (MQ=255)                                                                            gCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTg                                             <  2:559459/139‑1 (MQ=255)                                                                            gCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTg                                             <  1:276515/139‑1 (MQ=255)                                                                                 tttGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgtt                                                                                            <  1:648727/87‑1 (MQ=255)                                                                                 tttGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgtt                                                                                            >  2:648727/1‑87 (MQ=255)                                                                                     aCGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAat                                    >  2:82090/1‑139 (MQ=255)                                                                                                cagATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTgcgc                         >  2:381395/1‑139 (MQ=255)                                                                                                     aaaTCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGAttt                    >  1:1557110/1‑139 (MQ=255)                                                                                                            cGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGttt             >  2:1254110/1‑139 (MQ=255)                                                                                                             ggCAGCAACAGGTAGTTACAGATTCTGATAGAAGt                                                                                                                    >  2:1154810/1‑35 (MQ=255)                                                                                                             ggCAGCAACAGGTAGTTACAGATTCTGATAGAAGt                                                                                                                    <  1:1154810/35‑1 (MQ=255)                                                                                                                agcaACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCtt         >  1:72087/1‑139 (MQ=255)                                                                                                                 gcaACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                             >  1:747510/1‑38 (MQ=255)                                                                                                                 gcaACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                             <  2:747510/38‑1 (MQ=255)                                                                                                                    aCAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACt     >  1:724841/1‑139 (MQ=255)                                                                                                                       ggTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCa  <  1:1608909/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                      ATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCA  >  NC_000913/2130730‑2130986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑