Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	217,138	A→G	Y546Y (TAT→TAC)	proS ←	prolyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	217,138	0	A	G	100.0%	311.4 / NA	86	Y546Y (TAT→TAC)	proS	prolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/40);  total (46/40)

TTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAGCAGCACTTCGATACCT  >  NC_000913/217004‑217267                                                                                                                                       |                                                                                                                                  tttATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTAtt                                                                                                                               >  1:1600251/1‑139 (MQ=255)     taCAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGat                                                                                                                           <  1:168152/139‑1 (MQ=255)          tCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATAtcgt                                                                                                                      >  2:591042/1‑139 (MQ=255)          tCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATAtcgt                                                                                                                      >  2:796447/1‑139 (MQ=255)               gaaaaaaaaGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTGTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTg                                                                                                                 >  1:966311/3‑139 (MQ=255)               gTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTg                                                                                                                 >  1:1466143/1‑139 (MQ=255)               gTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTg                                                                                                                 >  1:973138/1‑139 (MQ=255)               gTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATCCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTg                                                                                                                 >  1:766439/1‑139 (MQ=255)                           cccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACgg                                                                                                     <  2:973138/139‑1 (MQ=255)                            ccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTAtt                                                                                                                               >  2:1548987/1‑112 (MQ=255)                            ccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTAtt                                                                                                                               <  1:1548987/112‑1 (MQ=255)                              tGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCg                                                                                                  <  2:590488/139‑1 (MQ=255)                                aaaTTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                <  2:1600251/139‑1 (MQ=255)                                aaaTTCAGGACCCTTTCTGGCATCAGCCCTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                <  2:966311/139‑1 (MQ=255)                                          cccTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATa                                                                                      <  2:1466143/139‑1 (MQ=255)                                          cccTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATa                                                                                      <  2:36436/139‑1 (MQ=255)                                                      tCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATa                                                                          >  2:900620/1‑139 (MQ=255)                                                          ccTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGa                                                                      >  2:62235/1‑139 (MQ=255)                                                          ccTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGa                                                                      <  2:1276044/139‑1 (MQ=255)                                                           cTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGAt                                                                     <  1:796447/139‑1 (MQ=255)                                                                    gTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                <  2:16366/103‑1 (MQ=255)                                                                    gTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                >  1:16366/1‑103 (MQ=255)                                                                    gTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCat                                                            >  1:899140/1‑139 (MQ=255)                                                                          ccAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGc                                                      >  1:1400991/1‑139 (MQ=255)                                                                          ccAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGc                                                      >  2:284394/1‑139 (MQ=255)                                                                             gATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                >  2:730695/1‑94 (MQ=255)                                                                             gATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                <  1:730695/94‑1 (MQ=255)                                                                             gATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATa                                                                          >  1:697518/1‑116 (MQ=255)                                                                             gATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCaaa                                                   <  2:899140/139‑1 (MQ=255)                                                                             gATATTCGACGATGTCACCAGTCTTAATTAAATGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATa                                                                          <  2:697518/116‑1 (MQ=255)                                                                              atatTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTc                                                                                                               >  1:365485/1‑78 (MQ=255)                                                                              atatTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTc                                                                                                               <  2:365485/78‑1 (MQ=255)                                                                              atatTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGAt                                                                     >  2:350442/1‑120 (MQ=255)                                                                              atatTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGAt                                                                     <  1:350442/120‑1 (MQ=255)                                                                               tatTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                <  1:1337758/92‑1 (MQ=255)                                                                               tatTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                >  2:1337758/1‑92 (MQ=255)                                                                                  tCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCa                                              >  2:684431/1‑139 (MQ=255)                                                                                         tGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCg                                                                                                  >  1:1476870/1‑80 (MQ=255)                                                                                         tGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCg                                                                                                  <  2:1476870/80‑1 (MQ=255)                                                                                         tGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCg                                                                                                  <  1:1381574/80‑1 (MQ=255)                                                                                         tGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCg                                                                                                  >  2:1381574/1‑80 (MQ=255)                                                                                          gTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGa                                      <  1:684431/139‑1 (MQ=255)                                                                                           tCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACgg                                                                                                     >  1:735767/1‑75 (MQ=255)                                                                                           tCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACgg                                                                                                     <  2:735767/75‑1 (MQ=255)                                                                                           tCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCg                                                                                                  <  1:775305/78‑1 (MQ=255)                                                                                           tCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCg                                                                                                  >  2:775305/1‑78 (MQ=255)                                                                                                    ttaattaaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATAtcg                                                                                                                       >  2:1165524/1‑48 (MQ=255)                                                                                                    ttaattaaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATAtcg                                                                                                                       <  1:1165524/48‑1 (MQ=255)                                                                                                    ttaattaaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGc                            <  1:509650/139‑1 (MQ=255)                                                                                                     taattaaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCg                                                                               <  2:119558/87‑1 (MQ=255)                                                                                                     taattaaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCg                                                                               >  1:119558/1‑87 (MQ=255)                                                                                                       attaaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                >  2:1568092/1‑68 (MQ=255)                                                                                                       attaaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                <  1:1568092/68‑1 (MQ=255)                                                                                                       attaaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGt                         >  1:499664/1‑139 (MQ=255)                                                                                                         taaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGAt                                                                     >  1:1597834/1‑93 (MQ=255)                                                                                                         taaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGAt                                                                     <  2:1597834/93‑1 (MQ=255)                                                                                                            cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCg                                                                       <  1:526954/88‑1 (MQ=255)                                                                                                            cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCg                                                                       >  2:526954/1‑88 (MQ=255)                                                                                                            cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGc                                   <  1:1594520/124‑1 (MQ=255)                                                                                                            cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGc                                   >  2:1594520/1‑124 (MQ=255)                                                                                                            cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATcc                    >  1:1301626/1‑139 (MQ=255)                                                                                                            cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATcc                    >  2:205704/1‑139 (MQ=255)                                                                                                                ttCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGtgtg                                                                                 >  1:4912/1‑74 (MQ=255)                                                                                                                ttCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGtgtg                                                                                 <  2:4912/74‑1 (MQ=255)                                                                                                                 tctcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                <  1:1388911/58‑1 (MQ=255)                                                                                                                 tctcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                >  2:1388911/1‑58 (MQ=255)                                                                                                                 tctcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGc                                                                                >  2:416444/1‑74 (MQ=255)                                                                                                                 tctcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGc                                                                                <  1:416444/74‑1 (MQ=255)                                                                                                                  ctcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGca                                                                                           >  1:1132573/1‑62 (MQ=255)                                                                                                                  ctcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGca                                                                                           <  2:1132573/62‑1 (MQ=255)                                                                                                                  ctcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTc                                                               >  2:415155/1‑90 (MQ=255)                                                                                                                  ctcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTc                                                               <  1:415155/90‑1 (MQ=255)                                                                                                                   tcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAgcagca             <  1:284394/139‑1 (MQ=255)                                                                                                                    cGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAGCAGCAc            >  1:475079/1‑139 (MQ=255)                                                                                                                         ttGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                >  2:1521443/1‑50 (MQ=255)                                                                                                                         ttGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                <  1:1521443/50‑1 (MQ=255)                                                                                                                         ttGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAGCAGCACTTCGa       >  1:438313/1‑139 (MQ=255)                                                                                                                         ttGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAGCAGCACTTCGa       >  2:51989/1‑139 (MQ=255)                                                                                                                         ttGCGACGATATTTGTATTAGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACACCCGGACGCTCTTTGCGGTCATCCAGCAGCACTTCGa       >  2:1412750/1‑139 (MQ=255)                                                                                                                          tGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACg                                    >  1:1443847/1‑109 (MQ=255)                                                                                                                          tGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACg                                    <  2:1443847/109‑1 (MQ=255)                                                                                                                           gCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCcagcag               >  1:1570034/1‑129 (MQ=255)                                                                                                                           gCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCcagcag               <  2:1570034/129‑1 (MQ=255)                                                                                                                              acgaTATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAGCAGCACTTCGATACCt  <  2:766439/139‑1 (MQ=255)                                                                                                                                   atTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCagcagc              <  1:1535833/122‑1 (MQ=255)                                                                                                                                   atTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCagcagc              >  2:1535833/1‑122 (MQ=255)                                                                                                                                       |                                                                                                                                  TTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAGCAGCACTTCGATACCT  >  NC_000913/217004‑217267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑