Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,179,330	T→C	S170S (AGT→AGC)	yegT →	nucleoside transporter, low affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,179,330	0	T	C	100.0%	274.8 / NA	76	S170S (AGT→AGC)	yegT	nucleoside transporter, low affinity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/38);  total (38/38)

TGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTT  >  NC_000913/2179195‑2179464                                                                                                                                        |                                                                                                                                        tGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGctct                                                                                                                                      >  1:1304191/1‑139 (MQ=255)  gATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCtctg                                                                                                                                     >  2:190807/1‑139 (MQ=255)       cccGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGctctgctctg                                                                                                                                >  2:72683/1‑139 (MQ=255)         cgcgCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTctgctctgct                                                                                                                              <  1:72683/139‑1 (MQ=255)            gcATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCgg                                                                                                                           >  2:1312490/1‑139 (MQ=255)                          ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑tttt                                                                                                            >  2:72094/1‑139 (MQ=255)                          ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑tttt                                                                                                            >  1:150280/1‑139 (MQ=255)                          ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACAGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑tttt                                                                                                            >  1:1151275/1‑139 (MQ=255)                             aCTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATTTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTcc                                                                                                         >  1:16851/1‑139 (MQ=255)                                      tGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGaca                                                                                                <  1:337351/139‑1 (MQ=255)                                      tGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGaca                                                                                                <  2:150280/139‑1 (MQ=255)                                             ccTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGccacc                                                                                         >  2:423137/1‑139 (MQ=255)                                                   gTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGgt                                                                                                                          <  1:1084910/101‑1 (MQ=255)                                                   gTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGgt                                                                                                                          >  2:1084910/1‑101 (MQ=255)                                                    tCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGc                                                                                  >  1:866346/1‑139 (MQ=255)                                                     cTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCa                                                                                 <  1:257233/139‑1 (MQ=255)                                                       ggCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCAcc                                                                               >  1:45976/1‑139 (MQ=255)                                                           tgtgGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCa                                                                           <  1:707773/139‑1 (MQ=255)                                                               gTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                       <  1:732365/139‑1 (MQ=255)                                                                tttCTTGCCGCAAATACTGGGTTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATg                                                                      <  2:1097634/139‑1 (MQ=255)                                                                     tGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGatat                                                                 <  2:1304191/139‑1 (MQ=255)                                                                          cAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAg                                                            >  1:277952/1‑139 (MQ=255)                                                                               aCTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATg                                                       <  1:1590151/139‑1 (MQ=255)                                                                                 tGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                     <  2:16851/139‑1 (MQ=255)                                                                                       aTGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCt                                               >  2:528189/1‑139 (MQ=255)                                                                                        tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCg                                                                                                                            <  1:643176/62‑1 (MQ=255)                                                                                        tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCg                                                                                                                            >  2:643176/1‑62 (MQ=255)                                                                                        tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTg                                              >  1:1406379/1‑139 (MQ=255)                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑t                                                                                                               <  2:278442/73‑1 (MQ=255)                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑t                                                                                                               >  1:278442/1‑73 (MQ=255)                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGa                                                                                                  <  2:112165/86‑1 (MQ=255)                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGa                                                                                                  >  1:112165/1‑86 (MQ=255)                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGc                                                                                             >  1:227299/1‑91 (MQ=255)                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGc                                                                                             <  2:227299/91‑1 (MQ=255)                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTgg                                             <  2:1105044/139‑1 (MQ=255)                                                                                           cGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGAt                                           >  2:359840/1‑139 (MQ=255)                                                                                             atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCtctg                                                                                                                                     >  2:143040/1‑48 (MQ=255)                                                                                             atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCtctg                                                                                                                                     <  1:143040/48‑1 (MQ=255)                                                                                             atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgc                                         <  2:1151275/139‑1 (MQ=255)                                                                                             atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgc                                         <  1:190807/139‑1 (MQ=255)                                                                                             atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgc                                         <  1:423137/139‑1 (MQ=255)                                                                                             atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgc                                         <  2:52708/139‑1 (MQ=255)                                                                                              tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGa                                                                                                  >  1:858502/1‑81 (MQ=255)                                                                                              tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGa                                                                                                  <  2:858502/81‑1 (MQ=255)                                                                                               atCTCACCGACTAACATCCCGCTGCTGATTACCGACGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgcgc                                       <  1:368183/139‑1 (MQ=255)                                                                                                   cACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGc                                                                                  <  2:212076/92‑1 (MQ=255)                                                                                                   cACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGc                                                                                  >  1:212076/1‑92 (MQ=255)                                                                                                    aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATc                                  >  2:329724/1‑139 (MQ=255)                                                                                                        aCTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgc                              >  1:1311129/1‑139 (MQ=255)                                                                                                        aCTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgc                              >  2:811289/1‑139 (MQ=255)                                                                                                          tAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCaaa                                                                         <  1:1533651/94‑1 (MQ=255)                                                                                                          tAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCaaa                                                                         >  2:1533651/1‑94 (MQ=255)                                                                                                          tAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgctg                            >  2:122039/1‑139 (MQ=255)                                                                                                          tAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgctg                            <  1:2395/139‑1 (MQ=255)                                                                                                            aCATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCGCCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTgcg                          >  2:1309064/1‑139 (MQ=255)                                                                                                            aCATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                     >  1:871638/1‑112 (MQ=255)                                                                                                            aCATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                     <  2:871638/112‑1 (MQ=255)                                                                                                                  cGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                       >  2:458935/1‑89 (MQ=255)                                                                                                                  cGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                       <  1:458935/89‑1 (MQ=255)                                                                                                                  cGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGaca                                                                                                >  1:296884/1‑63 (MQ=255)                                                                                                                  cGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGaca                                                                                                <  2:296884/63‑1 (MQ=255)                                                                                                                   gctgctGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGcca                                                                                           >  1:115890/1‑67 (MQ=255)                                                                                                                   gctgctGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGcca                                                                                           <  2:115890/67‑1 (MQ=255)                                                                                                                    ctgctgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGt                                                           <  1:1111336/98‑1 (MQ=255)                                                                                                                    ctgctgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGt                                                           >  2:1111336/1‑98 (MQ=255)                                                                                                                    ctgctgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGcc                                                >  1:18304/1‑109 (MQ=255)                                                                                                                    ctgctgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGcc                                                <  2:18304/109‑1 (MQ=255)                                                                                                                        tgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACtt              >  2:741061/1‑139 (MQ=255)                                                                                                                          aTTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTcc            <  1:741061/139‑1 (MQ=255)                                                                                                                          aTTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTcc            >  1:1510797/1‑139 (MQ=255)                                                                                                                             aCCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg         <  1:329724/139‑1 (MQ=255)                                                                                                                             aCCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg         <  2:277952/139‑1 (MQ=255)                                                                                                                             aCCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg         <  2:45976/139‑1 (MQ=255)                                                                                                                              ccgccgGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTa                                                               >  2:1301033/1‑84 (MQ=255)                                                                                                                              ccgccgGAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTa                                                               <  1:1301033/84‑1 (MQ=255)                                                                                                                                    gAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCAc                                                                                <  1:1592502/61‑1 (MQ=255)                                                                                                                                    gAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCAc                                                                                >  2:1592502/1‑61 (MQ=255)                                                                                                                                    gAAGCTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCttttt  >  1:236551/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        TGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCG‑TTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTT  >  NC_000913/2179195‑2179464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑