| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,338,234 | A→G | L396P (CTG→CCG) | gyrA ← | DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,338,234 | 0 | A | G | 94.1% | 106.1 / ‑2.1 | 34 | L396P (CTG→CCG) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (12/20); total (13/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01 | |||||||||||
AGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCAGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGAC > NC_000913/2338111‑2338354 | aGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGt < 1:17320/139‑1 (MQ=255)aGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGt < 1:414913/139‑1 (MQ=255) cgcAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGGAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGAGCGGGTCGATGTTCGCCAgc > 1:1053513/1‑139 (MQ=255) cagcaTCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGt < 2:274017/123‑1 (MQ=255) cagcaTCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGt > 1:274017/1‑123 (MQ=255) gtcgCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCt < 2:108662/139‑1 (MQ=255) cGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCAc > 2:488004/1‑121 (MQ=255) cGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCAc < 1:488004/121‑1 (MQ=255) aTCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGGCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAg < 2:1053513/139‑1 (MQ=255) cgGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCAc < 1:1158253/112‑1 (MQ=255) cgGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCAc > 2:1158253/1‑112 (MQ=255) aCGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATcgc < 2:976338/139‑1 (MQ=255) aCGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGATCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATcgc < 2:590581/139‑1 (MQ=255) cTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGc < 2:362649/139‑1 (MQ=255) ccACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGt > 1:187166/1‑139 (MQ=255) gATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGc < 2:776596/91‑1 (MQ=255) gATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGc > 1:776596/1‑91 (MQ=255) gATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGaa < 2:490702/139‑1 (MQ=255) ccAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCaa > 1:509340/1‑93 (MQ=255) ccAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCaa < 2:509340/93‑1 (MQ=255) aGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGc < 2:387278/120‑1 (MQ=255) aGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGc > 1:387278/1‑120 (MQ=255) aGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTacgac > 1:1449853/1‑139 (MQ=255) gcgcAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTacgacg < 2:1449853/139‑1 (MQ=255) cgcgGTTTTCGCTTCTGCAGGCGTCGGCTCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACgg < 2:672612/139‑1 (MQ=255) gCTTCTGCAGGCGTCGGCGCATGACGGATCAGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTc > 1:118358/1‑113 (MQ=255) gCTTCTGCAGGCGTCGGCGCATGACGGATCAGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTc < 2:118358/113‑1 (MQ=255) tGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTGCGCAGTTCGAAAATAGTACGACGGGTCACCACTTCAcgg < 2:523589/139‑1 (MQ=255) tGCAGGCGTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCAcgg < 1:765686/139‑1 (MQ=255) gTCGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGAc > 2:261997/1‑139 (MQ=255) cGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATcgc > 2:145621/1‑83 (MQ=255) cGGCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATcgc < 1:145621/83‑1 (MQ=255) ggCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTaa < 2:3400/56‑1 (MQ=255) ggCGCATGACGGATCGGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTaa > 1:3400/1‑56 (MQ=255) | AGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCAGTTCGATGATCGGGTCGATGTTCGCCAGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGAC > NC_000913/2338111‑2338354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |