Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,521,852 T→C D209G (GAT→GGT)  xapR ← transcriptional activator of xapAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,521,8520TC98.4% 215.7 / ‑5.6 61D209G (GAT→GGT) xapRtranscriptional activator of xapAB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (26/34);  minor base A (1/0);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAG  >  NC_000913/2521719‑2521986
                                                                                                                                     |                                                                                                                                      
ttGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcag                                                                                                                                   >  2:198701/1‑139 (MQ=255)
 tgtgCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                                  <  1:1402887/139‑1 (MQ=255)
 tgtgCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                                  <  2:858412/139‑1 (MQ=255)
 tgtgCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                                  <  2:814147/139‑1 (MQ=255)
 tgtgCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                                  <  2:177246/139‑1 (MQ=255)
 tgtgCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                                  <  1:880426/139‑1 (MQ=255)
  gtgCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagtc                                                                                                                                 <  1:85681/139‑1 (MQ=255)
   tgCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagtca                                                                                                                                <  2:87821/139‑1 (MQ=255)
        aGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTa                                                                                                                           >  1:433901/1‑139 (MQ=255)
            gTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACg                                                                                                                       <  2:1342066/139‑1 (MQ=255)
                      aaTGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTg                                                                                                             <  2:818670/139‑1 (MQ=255)
                       aTGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGt                                                                                                            <  2:975299/139‑1 (MQ=255)
                                 aaTGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATAc                                                                                                  <  2:139290/139‑1 (MQ=255)
                                  aTGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACt                                                                                                 >  1:1506897/1‑139 (MQ=255)
                                        aTACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCAc                                                                                           <  1:5424/139‑1 (MQ=255)
                                              aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCca                                                                                                                                    >  2:798511/1‑92 (MQ=255)
                                              aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCca                                                                                                                                    <  1:798511/92‑1 (MQ=255)
                                              aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCTGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAgcg                                                                                     >  2:1455678/1‑139 (MQ=255)
                                              aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                                >  2:5166/1‑112 (MQ=255)
                                              aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAgcg                                                                                     >  2:361133/1‑139 (MQ=255)
                                              aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGCTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                                <  1:5166/112‑1 (MQ=255)
                                                cATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCt                                                                                   <  2:1256428/139‑1 (MQ=255)
                                                         tACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCggg                                                                          >  1:713565/1‑139 (MQ=255)
                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGa                                                                         >  2:426178/1‑139 (MQ=255)
                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGa                                                                         >  2:560577/1‑139 (MQ=255)
                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGa                                                                         <  1:426178/139‑1 (MQ=255)
                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAAACCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGa                                                                         >  1:1033747/1‑139 (MQ=255)
                                                              tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAACAAACCGCAGTCAGTGTAAACGGGCGGCATTGCAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGt                                                                     >  2:117560/1‑139 (MQ=255)
                                                              tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTa                                                                                                           <  1:731969/101‑1 (MQ=255)
                                                              tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTa                                                                                                           >  2:731969/1‑101 (MQ=255)
                                                              tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGt                                                                     >  1:627297/1‑139 (MQ=255)
                                                              tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGt                                                                     <  2:627297/139‑1 (MQ=255)
                                                              tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGt                                                                     >  1:363205/1‑139 (MQ=255)
                                                               ttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTg                                                                    >  2:567252/1‑139 (MQ=255)
                                                                            aCTTCGCGAATAACAACCGGTGAAAATCCTACCTGCTGGCAAACTAGCTGCAGAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCggg                                                                          >  1:932201/1‑120 (MQ=255)
                                                                            aCTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCggg                                                                          <  2:932201/120‑1 (MQ=255)
                                                                                cgcgAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACg                                                                       >  1:199390/1‑119 (MQ=255)
                                                                                cgcgAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACg                                                                       <  2:199390/119‑1 (MQ=255)
                                                                                 gcgAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAgatga                                                  >  1:991112/1‑139 (MQ=255)
                                                                                 gcgAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAgatga                                                  <  1:198701/139‑1 (MQ=255)
                                                                                      tAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGctc                                             >  2:664099/1‑139 (MQ=255)
                                                                                               gttaaaaTCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTg                                                                                                             <  1:1574201/63‑1 (MQ=255)
                                                                                               gttaaaaTCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTg                                                                                                             >  2:1574201/4‑66 (MQ=255)
                                                                                               gTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACaaa                                                                                                      <  1:1436/73‑1 (MQ=255)
                                                                                               gTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACaaa                                                                                                      >  2:1436/1‑73 (MQ=255)
                                                                                                         cACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGa                          >  2:739363/1‑139 (MQ=255)
                                                                                                          aCCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                                <  2:1517201/52‑1 (MQ=255)
                                                                                                          aCCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                                >  1:1517201/1‑52 (MQ=255)
                                                                                                             tgctgGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTTTAACAAAATACTCGTCACGTAGCACTTCCAGCGGGACGGTGGAAAATGATTAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACg                      <  2:1023968/139‑1 (MQ=255)
                                                                                                              gctgGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGc                     <  2:991112/139‑1 (MQ=255)
                                                                                                                 ggCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGa                  >  2:268332/1‑139 (MQ=255)
                                                                                                                       cTCGCTGCAAAAAACCCCTGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATg            <  1:1455678/139‑1 (MQ=255)
                                                                                                                       cTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATg            <  2:713565/139‑1 (MQ=255)
                                                                                                                       cTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATg            <  2:1053181/139‑1 (MQ=255)
                                                                                                                       cTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATg            <  1:560577/139‑1 (MQ=255)
                                                                                                                        tCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGc           <  2:1474394/139‑1 (MQ=255)
                                                                                                                          gCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTg                                                                                                             <  1:951085/39‑1 (MQ=255)
                                                                                                                          gCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTg                                                                                                             >  2:951085/1‑39 (MQ=255)
                                                                                                                            tGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAAcc       >  2:475376/1‑139 (MQ=255)
                                                                                                                                 aaaaCCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAg  <  1:664099/139‑1 (MQ=255)
                                                                                                                                 aaaaCCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAg  <  1:361133/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAG  >  NC_000913/2521719‑2521986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: