Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,525,930 C→T *309* (TAG→TAA yfeR ← transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,9300CT100.0% 188.1 / NA 59*309* (TAG→TAAyfeRtranscriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/28);  total (31/28)

TACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCA  >  NC_000913/2525801‑2526054
                                                                                                                                 |                                                                                                                            
cacacattactacgggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGa                                                                                                                     <  2:639328‑M2/14‑1 (MQ=255)
   cattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                                  <  1:1123064‑M2/17‑1 (MQ=255)
   cattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                                  <  2:769061‑M2/17‑1 (MQ=255)
                  tcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTcc                                                                                                   >  2:1535023‑M2/108‑139 (MQ=255)
                           atatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGcc                                                                                          >  1:566451‑M2/99‑139 (MQ=255)
                                cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAg                                                                                     >  2:278324‑M2/94‑139 (MQ=255)
                                cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAg                                                                                     >  1:352167‑M2/94‑139 (MQ=255)
                                    tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                 <  1:278324‑M2/50‑1 (MQ=255)
                                     gacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                >  2:404576‑M2/89‑139 (MQ=255)
                                     gacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                >  1:644168‑M2/89‑139 (MQ=255)
                                          actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                           <  1:107166‑M2/56‑1 (MQ=255)
                                          actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                           <  1:1535023‑M2/56‑1 (MQ=255)
                                          actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                           <  2:823701‑M2/56‑1 (MQ=255)
                                            tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                         <  2:21542‑M2/58‑1 (MQ=255)
                                             gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTatat                                                                                                                         >  2:773522‑M2/81‑90 (MQ=255)
                                             gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTatat                                                                                                                         <  1:773522‑M2/10‑1 (MQ=255)
                                                                    cgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcc                                                                                                         <  2:679982‑M2/26‑1 (MQ=255)
                                                                    cgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcc                                                                                                         >  1:679982‑M2/58‑83 (MQ=255)
                                                                    cgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                         >  2:911291‑M2/58‑115 (MQ=255)
                                                                    cgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                         <  1:911291‑M2/58‑1 (MQ=255)
                                                                    cgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGc                                                 >  1:634340‑M2/58‑139 (MQ=255)
                                                                          tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCACCCAGCCATTAGCGCATTGCCCTGGTCTCg                                                                   <  1:203720‑M2/64‑1 (MQ=255)
                                                                          tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCACCCAGCCATTAGCGCATTGCCCTGGTCTCg                                                                   >  2:203720‑M2/52‑115 (MQ=255)
                                                                           ctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                          >  2:1112982‑M2/51‑59 (MQ=255)
                                                                           ctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                          <  1:1112982‑M2/9‑1 (MQ=255)
                                                                            tactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcgg                                         <  1:505284‑M2/90‑1 (MQ=255)
                                                                                 catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                                    >  1:166339‑M2/45‑139 (MQ=255)
                                                                                 catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                                    >  2:173823‑M2/45‑139 (MQ=255)
                                                                                   tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                  >  2:686724‑M2/43‑139 (MQ=255)
                                                                                   tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                  >  2:388806‑M2/43‑139 (MQ=255)
                                                                                   tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                  >  2:325300‑M2/43‑139 (MQ=255)
                                                                                   tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                  >  2:1358837‑M2/43‑139 (MQ=255)
                                                                                        tttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg                             <  1:325300‑M2/102‑1 (MQ=255)
                                                                                         ttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                            <  1:1358837‑M2/103‑1 (MQ=255)
                                                                                         ttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                            <  1:173823‑M2/103‑1 (MQ=255)
                                                                                           aacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                        <  1:1517039‑M2/11‑1 (MQ=255)
                                                                                           aacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                        >  2:1517039‑M2/35‑45 (MQ=255)
                                                                                            acttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACg                         <  1:388806‑M2/106‑1 (MQ=255)
                                                                                                 tgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                           >  1:109027‑M2/29‑84 (MQ=255)
                                                                                                 tgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                           <  2:109027‑M2/56‑1 (MQ=255)
                                                                                                      gattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg               <  2:634340‑M2/116‑1 (MQ=255)
                                                                                                        ttactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt                       <  2:991231‑M2/108‑1 (MQ=255)
                                                                                                        ttactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt                       >  1:991231‑M2/22‑129 (MQ=255)
                                                                                                        ttactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg             >  2:1241514‑M2/22‑139 (MQ=255)
                                                                                                          actgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa           >  2:1164888‑M2/20‑139 (MQ=255)
                                                                                                              ccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                        >  2:1168022‑M2/16‑58 (MQ=255)
                                                                                                              ccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                        <  1:1168022‑M2/43‑1 (MQ=255)
                                                                                                                 gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                 >  1:821947‑M2/13‑62 (MQ=255)
                                                                                                                 gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                 <  2:821947‑M2/50‑1 (MQ=255)
                                                                                                                 gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCAt    >  2:71942‑M2/13‑139 (MQ=255)
                                                                                                                   gctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTAGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCa  >  2:1003789‑M2/11‑139 (MQ=255)
                                                                                                                     tcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                           >  1:1456587‑M2/9‑64 (MQ=255)
                                                                                                                     tcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                           <  2:1456587‑M2/56‑1 (MQ=255)
                                                                                                                      cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                              <  2:121788‑M2/37‑1 (MQ=255)
                                                                                                                      cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                              >  1:121788‑M2/8‑44 (MQ=255)
                                                                                                                      cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                            <  1:983780‑M2/87‑1 (MQ=255)
                                                                                                                      cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                            >  2:983780‑M2/8‑94 (MQ=255)
                                                                                                                        cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa                           >  2:644255‑M2/6‑109 (MQ=255)
                                                                                                                        cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa                           <  1:644255‑M2/104‑1 (MQ=255)
                                                                                                                                 |                                                                                                                            
TACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCA  >  NC_000913/2525801‑2526054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: