Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,697,915	C→T	*212* (TAG→TAA)	pgpC ←	phosphatidylglycerophosphatase C, membrane bound

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,697,915	0	C	T	100.0%	148.7 / NA	47	*212* (TAG→TAA)	pgpC	phosphatidylglycerophosphatase C, membrane bound
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/22);  total (25/22)

CGCTCATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCT  >  NC_000913/2697781‑2698045                                                                                                                                       |                                                                                                                                        cGCTCATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTAtt                                                                                                                                     <  1:456657/139‑1 (MQ=255)   cTCATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTcc                                                                                                                                   <  1:10778/139‑1 (MQ=255)             gCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGa                                                                                                                         >  1:1423429/1‑139 (MQ=255)                  tttCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAGAACGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                                    >  1:568947/1‑139 (MQ=255)                                                 tATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGAc                                                                                     >  2:262539/1‑139 (MQ=255)                                                 tATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGAc                                                                                     <  2:1423429/139‑1 (MQ=255)                                                 tATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGATGGAGTTCACCGCGCTGGGTTACTCGCCAACGCTGCTGAc                                                                                     <  2:982539/139‑1 (MQ=255)                                                      gTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGAAGCTGACAGaaa                                                                                >  2:527443/1‑139 (MQ=255)                                                       tGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAAt                                                                               >  2:600452/1‑139 (MQ=255)                                                        ggCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAg                                                                                                                                 <  1:1107446/88‑1 (MQ=255)                                                        ggCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAg                                                                                                                                 >  2:1107446/1‑88 (MQ=255)                                                          cTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATaaa                                                                            <  1:1178268/139‑1 (MQ=255)                                                           tAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAag                                                                           >  2:342903/1‑139 (MQ=255)                                                           tAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAag                                                                           >  2:1454973/1‑139 (MQ=255)                                                                  gACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGa                                                                    <  1:1454973/139‑1 (MQ=255)                                                                    cTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                                  <  2:341506/139‑1 (MQ=255)                                                                       cAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTc                                                               >  2:774302/1‑139 (MQ=255)                                                                        aGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                               <  1:1532408/90‑1 (MQ=255)                                                                        aGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                               >  2:1532408/1‑90 (MQ=255)                                                                                 gAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTg                                                     <  2:568947/139‑1 (MQ=255)                                                                                        ccGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCtat                                              <  1:262539/139‑1 (MQ=255)                                                                                        ccGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCtat                                              <  1:774302/139‑1 (MQ=255)                                                                                             tataAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGAAGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑a                                    <  1:527443/139‑1 (MQ=255)                                                                                                     cgcgGCGCATTATACATGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACg                                                                                             >  1:695169/1‑79 (MQ=255)                                                                                                     cgcgGCGCATTATACATGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACg                                                                                             <  2:695169/79‑1 (MQ=255)                                                                                                     cgcgGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAg                            >  2:791481/1‑139 (MQ=255)                                                                                                          cgcATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                               >  2:569536/1‑56 (MQ=255)                                                                                                          cgcATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                               <  1:569536/56‑1 (MQ=255)                                                                                                             aTTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCg                    <  1:1383336/139‑1 (MQ=255)                                                                                                                      ggACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCt                                                             <  1:458989/94‑1 (MQ=255)                                                                                                                      ggACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCt                                                             >  2:458989/1‑94 (MQ=255)                                                                                                                      ggACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCGGAGTGCCGa           >  2:428855/1‑139 (MQ=255)                                                                                                                       gACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGa                                                                    >  2:264543/1‑86 (MQ=255)                                                                                                                       gACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGa                                                                    <  1:264543/86‑1 (MQ=255)                                                                                                                        aCGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCGGAGTGCCGAtt         >  1:1287639/1‑139 (MQ=255)                                                                                                                         cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCgggg                                                                                                          <  1:604455/46‑1 (MQ=255)                                                                                                                         cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCgggg                                                                                                          >  2:604455/1‑46 (MQ=255)                                                                                                                         cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCGGAGTGCCGAttt        >  2:450253/1‑139 (MQ=255)                                                                                                                           cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGa                                                                    >  2:664915/1‑82 (MQ=255)                                                                                                                           cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGa                                                                    <  1:664915/82‑1 (MQ=255)                                                                                                                           cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCGGAGTGCCGAttt        >  1:63813/1‑137 (MQ=255)                                                                                                                           cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCGGAGTGCCGAttt        <  2:63813/137‑1 (MQ=255)                                                                                                                           cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCGGAGTGCCGATTTTg      >  1:787508/1‑139 (MQ=255)                                                                                                                              tGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCg                    <  2:379217/122‑1 (MQ=255)                                                                                                                              tGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCg                    >  1:379217/1‑122 (MQ=255)                                                                                                                               gCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCt  >  2:4181/1‑139 (MQ=255)                                                                                                                                  ttACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACTGTACAGCCGCAGCGGAGTGCCGATTTTgcg    >  2:1527235/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        CGCTCATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCC‑‑‑‑‑ACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCT  >  NC_000913/2697781‑2698045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑