Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,839,384	T→A	intergenic (‑120/‑140)	ascG ← / → ascF	asc operon transcriptional repressor, prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,839,384	0	T	A	100.0%	195.8 / NA	61	intergenic (‑120/‑140)	ascG/ascF	asc operon transcriptional repressor, prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (29/32);  total (29/32)

CCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATA  >  NC_000913/2839249‑2839516                                                                                                                                        |                                                                                                                                     ccAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCa                                                                                                                                   <  2:1211592/139‑1 (MQ=255) ccAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCa                                                                                                                                   <  1:222393/139‑1 (MQ=255)         gtcgtgATCATTTTCAACCCTTTACACGCAATCCACGTAGTGTACTGCAGCCTTTTCCGATTGTCCTTACACACTCGGCGGGAATAATATTCAAGTGCCCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTg                                                                                                                           <  1:848984/139‑1 (MQ=255)         gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCAct                                                                                                                                 <  1:543353/133‑1 (MQ=255)         gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCAct                                                                                                                                 >  2:543353/1‑133 (MQ=255)          tcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGc                                                                                                                          >  2:986338/1‑139 (MQ=255)           cgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCt                                                                                                                         >  1:735769/1‑139 (MQ=255)                 catTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTa                                                                                                                   <  1:666225/139‑1 (MQ=255)                        aTCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACaa                                                                                                            <  1:1471177/139‑1 (MQ=255)                        aTCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACaa                                                                                                            <  1:986338/139‑1 (MQ=255)                              ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCt                                                                                                                         <  1:204146/120‑1 (MQ=255)                              ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCt                                                                                                                         >  2:204146/1‑120 (MQ=255)                                           aCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaa                                                                                         <  2:735769/139‑1 (MQ=255)                                                gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgg                                                                                    >  1:96730/1‑139 (MQ=255)                                                gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgg                                                                                    <  1:1067293/139‑1 (MQ=255)                                                      tGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTAt                                                                                                                      <  2:732736/99‑1 (MQ=255)                                                      tGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTAt                                                                                                                      >  1:732736/1‑99 (MQ=255)                                                        cACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGt                                                                                   >  2:1592642/1‑132 (MQ=255)                                                        cACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGt                                                                                   <  1:1592642/132‑1 (MQ=255)                                                         aCCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAt                                                                           <  1:45193/139‑1 (MQ=255)                                                           cGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTc                                                                         >  1:1145003/1‑139 (MQ=255)                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTAt                                                                                                                      <  2:947745/92‑1 (MQ=255)                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTAt                                                                                                                      >  1:947745/1‑92 (MQ=255)                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACt                                                                                                          <  1:623071/104‑1 (MQ=255)                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACt                                                                                                          >  2:623071/1‑104 (MQ=255)                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCg                                                                       >  1:649306/1‑139 (MQ=255)                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCg                                                                       >  1:190542/1‑139 (MQ=255)                                                                gCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActct                                                                                                                               <  2:235774/80‑1 (MQ=255)                                                                gCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActct                                                                                                                               >  1:235774/1‑80 (MQ=255)                                                                           ttGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa                                                         <  2:96730/139‑1 (MQ=255)                                                                              cacaATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAt                                                                           <  1:1560692/118‑1 (MQ=255)                                                                              cacaATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAt                                                                           >  2:1560692/1‑118 (MQ=255)                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCa                                                                                                                                   >  1:639343/1‑57 (MQ=255)                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCa                                                                                                                                   <  2:639343/57‑1 (MQ=255)                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActctct                                                                                                                             >  1:405810/1‑63 (MQ=255)                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActctct                                                                                                                             <  2:405810/63‑1 (MQ=255)                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActct                                                                                                                               <  2:457408/61‑1 (MQ=255)                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActct                                                                                                                               >  1:457408/1‑61 (MQ=255)                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGAc                                                                                                              >  2:897536/1‑78 (MQ=255)                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGAc                                                                                                              <  1:897536/78‑1 (MQ=255)                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACaa                                                                                                            >  1:867033/1‑80 (MQ=255)                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACaa                                                                                                            <  2:867033/80‑1 (MQ=255)                                                                                     ggcggGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAt                                                                           >  2:526056/1‑111 (MQ=255)                                                                                     ggcggGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAt                                                                           <  1:526056/111‑1 (MQ=255)                                                                                            aaaaTATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGa                                        <  2:499032/139‑1 (MQ=255)                                                                                                     aGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCAct                                                                                                                                 <  1:736740/41‑1 (MQ=255)                                                                                                     aGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCAct                                                                                                                                 >  2:736740/1‑41 (MQ=255)                                                                                                        tGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACt                            >  1:827743/1‑139 (MQ=255)                                                                                                          aCCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCAt                                                              >  2:1551406/1‑103 (MQ=255)                                                                                                          aCCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCAt                                                              <  1:1551406/103‑1 (MQ=255)                                                                                                          aCCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTAc                          <  2:827743/139‑1 (MQ=255)                                                                                                            cGGTTTCACAAATATAAAAAATGAACAAATCActct                                                                                                                               <  1:887596/36‑1 (MQ=255)                                                                                                            cGGTTTCACAAATATAAAAAATGAACAAATCActct                                                                                                                               >  2:887596/1‑36 (MQ=255)                                                                                                            cGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACAt                        >  2:527411/1‑139 (MQ=255)                                                                                                                    caAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTa                <  1:527411/139‑1 (MQ=255)                                                                                                                     aaaTATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTAc               >  1:1157090/1‑139 (MQ=255)                                                                                                                         ataAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCAt                                                              <  1:1437812/88‑1 (MQ=255)                                                                                                                         ataAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCAt                                                              >  2:1437812/1‑88 (MQ=255)                                                                                                                         ataAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGAt           <  2:1157090/139‑1 (MQ=255)                                                                                                                             aaaaTGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACa       >  2:1370599/1‑139 (MQ=255)                                                                                                                                  gAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATa  >  1:1218687/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     CCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATA  >  NC_000913/2839249‑2839516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑