| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,899,264 | G→A | A52V (GCA→GTA) | ygcU ← | putative FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,899,264 | 0 | G | A | 98.7% | 242.3 / ‑4.4 | 76 | A52V (GCA→GTA) | ygcU | putative FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (39/36); total (40/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
ACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGG > NC_000913/2899138‑2899389 | acaacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa < 1:667046/139‑1 (MQ=255)acaacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa < 1:147930/139‑1 (MQ=255) aacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCaaa > 1:74077/1‑139 (MQ=255) tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAAt > 2:812108/1‑127 (MQ=255) tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAAt < 1:812108/127‑1 (MQ=255) tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCaa < 1:730501/133‑1 (MQ=255) tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCaa > 2:730501/1‑133 (MQ=255) tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata > 2:443678/1‑139 (MQ=255) tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata > 1:902371/1‑139 (MQ=255) cccACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCa > 1:110556/1‑139 (MQ=255) ccACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCAt > 2:519195/1‑139 (MQ=255) aCCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGa > 2:262599/1‑139 (MQ=255) aCCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGa > 1:572391/1‑139 (MQ=255) cTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAat > 1:178979/1‑139 (MQ=255) gTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATTCTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCg > 2:1273993/1‑139 (MQ=255) gTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCg > 1:375711/1‑139 (MQ=255) gAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAAtt < 2:629002/139‑1 (MQ=255) cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATTCTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa < 1:1273993/139‑1 (MQ=255) cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa < 1:519195/139‑1 (MQ=255) cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa < 1:262599/139‑1 (MQ=255) cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa < 2:613273/139‑1 (MQ=255) cgcgGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt < 1:1028960/139‑1 (MQ=255) cacaCCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCgg < 1:669505/90‑1 (MQ=255) cacaCCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCgg > 2:669505/1‑90 (MQ=255) acacCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATAc < 2:902371/139‑1 (MQ=255) acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata < 1:579358/96‑1 (MQ=255) acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata > 2:579358/1‑96 (MQ=255) cGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGtt > 1:835703/1‑139 (MQ=255) gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt < 2:651352/139‑1 (MQ=255) gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt < 2:572391/139‑1 (MQ=255) aaTTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa < 2:179702/83‑1 (MQ=255) aaTTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa > 1:179702/1‑83 (MQ=255) gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa > 1:1304933/1‑77 (MQ=255) gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa < 2:1304933/77‑1 (MQ=255) gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata > 1:1503154/1‑84 (MQ=255) gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata < 2:1503154/84‑1 (MQ=255) gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATaa < 1:1614144/85‑1 (MQ=255) gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATaa > 2:1614144/1‑85 (MQ=255) gcgcATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATAc > 2:671693/1‑139 (MQ=255) aTTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt > 1:1272410/1‑113 (MQ=255) aTTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt < 2:1272410/113‑1 (MQ=255) aTTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGtt < 1:1310523/139‑1 (MQ=255) aTTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGtt > 2:1310523/1‑139 (MQ=255) aTAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCAt < 1:443678/139‑1 (MQ=255) tAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATc > 2:278137/1‑139 (MQ=255) ttCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt < 1:1023392/114‑1 (MQ=255) ttCAGCACACGGGATACTTGCTCTGTGGAACCGAGTGTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt > 2:1023392/1‑114 (MQ=255) aGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt > 2:778775/1‑111 (MQ=255) aGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt < 1:778775/111‑1 (MQ=255) gggATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATc > 2:1289961/1‑139 (MQ=255) tGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaa > 2:76090/1‑139 (MQ=255) ctctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCaa < 1:13006/42‑1 (MQ=255) ctctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCaa > 2:13006/1‑42 (MQ=255) ctctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTAc < 1:485616/123‑1 (MQ=255) ctctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTAc > 2:485616/1‑123 (MQ=255) tctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa < 2:835703/139‑1 (MQ=255) tctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa < 1:1289961/139‑1 (MQ=255) tctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa < 1:190366/139‑1 (MQ=255) ctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGt > 1:1302100/1‑90 (MQ=255) ctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGt < 2:1302100/90‑1 (MQ=255) ctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAAt > 2:192454/1‑139 (MQ=255) gtgGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa > 1:1409088/1‑136 (MQ=255) gtgGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa < 2:1409088/136‑1 (MQ=255) tgGAACCGAGTTTTACGACCGCTACCGGAATCGGCa < 1:1264959/36‑1 (MQ=38) tgGAACCGAGTTTTACGACCGCTACCGGAATCGGCa > 2:1264959/1‑36 (MQ=255) tgGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt > 2:234925/1‑89 (MQ=255) tgGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt < 1:234925/89‑1 (MQ=255) aGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCt > 1:604601/1‑139 (MQ=255) ttttACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATAc < 1:603471/74‑1 (MQ=255) ttttACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATAc > 2:603471/1‑74 (MQ=255) ttttACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTgg > 2:1499574/1‑139 (MQ=255) ttttACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTgg > 2:824786/1‑139 (MQ=255) cGACCGCTACCGGAATCGGCAAAGTATAAATGCCAt < 2:1117905/36‑1 (MQ=255) cGACCGCTACCGGAATCGGCAAAGTATAAATGCCAt > 1:1117905/1‑36 (MQ=255) gCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCa > 2:1504072/1‑87 (MQ=255) gCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCa < 1:1504072/87‑1 (MQ=255) | ACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGG > NC_000913/2899138‑2899389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |