Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,288	C→T	*279* (TAG→TAA)	kduI ←	hexuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,288	0	C	T	100.0%	250.4 / NA	77	*279* (TAG→TAA)	kduI	hexuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/36);  total (41/36)

ACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCA  >  NC_000913/2983154‑2983415                                                                                                                                       |                                                                                                                                acaGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAgc                                                                                                                             >  1:838495/1‑139 (MQ=255)                cAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACgg                                                                                                              <  2:838495/139‑1 (MQ=255)                   ccccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCca                                                                                                           <  2:311562/139‑1 (MQ=255)                    cccAACGCCATCACCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCTCAATTAGCGCAAATCTTTAACGGCcac                                                                                                          <  1:960689/139‑1 (MQ=255)                       aaCGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATg                                                                                                       <  2:74896/139‑1 (MQ=255)                                    gACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATAtcatca                                                                                          >  1:1102726/1‑139 (MQ=255)                                          gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGt                                                                                                     >  2:307992/1‑122 (MQ=255)                                          gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGt                                                                                                     <  1:307992/122‑1 (MQ=255)                                          gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAcc                                                                                    >  2:438284/1‑139 (MQ=255)                                           tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                         <  2:6580/117‑1 (MQ=255)                                           tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                         >  1:6580/1‑117 (MQ=255)                                             cagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTgg                                                                                 <  1:438284/139‑1 (MQ=255)                                              agtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGt                                                                                <  2:1102726/139‑1 (MQ=255)                                                tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                                          <  1:1009668/111‑1 (MQ=255)                                                tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                                          >  2:1009668/1‑111 (MQ=255)                                                tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGttt                                                                              >  1:787401/1‑139 (MQ=255)                                                     caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                     >  2:906589/1‑127 (MQ=255)                                                     caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                     >  2:362003/1‑127 (MQ=255)                                                     caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                     <  1:362003/127‑1 (MQ=255)                                                     caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                     <  1:906589/127‑1 (MQ=255)                                                         cAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                     >  1:512950/1‑139 (MQ=255)                                                          aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                         <  2:311278/102‑1 (MQ=255)                                                          aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                         >  1:311278/1‑102 (MQ=255)                                                            tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAgagc                                                                                                                           <  1:432262/82‑4 (MQ=255)                                                            tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAgagc                                                                                                                           >  2:432262/1‑82 (MQ=255)                                                              acgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                <  2:1587408/139‑1 (MQ=255)                                                                gacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                         >  2:186241/1‑96 (MQ=255)                                                                gacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                         <  1:186241/96‑1 (MQ=255)                                                                    gCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAc                                                                          <  2:1536766/123‑1 (MQ=255)                                                                    gCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAc                                                                          >  1:1536766/1‑123 (MQ=255)                                                                     cAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                         <  2:512950/139‑1 (MQ=255)                                                                            aCCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTc                                                                                                    <  2:1168366/89‑1 (MQ=255)                                                                            aCCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTc                                                                                                    >  1:1168366/1‑89 (MQ=255)                                                                                            gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                     <  2:1019988/88‑1 (MQ=255)                                                                                            gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATAGCATCAAAGAc                                                                                     >  1:1019988/1‑88 (MQ=255)                                                                                               cTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACg                                                                                                               <  1:998904/59‑1 (MQ=255)                                                                                               cTTAAAATCATAACAATACCTTATTCGTGTATGCCCACAATTAGCGCAAATCTTTAACg                                                                                                               >  2:998904/1‑59 (MQ=255)                                                                                                ttAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACgg                                                                                                              >  1:1126633/1‑59 (MQ=255)                                                                                                ttAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACgg                                                                                                              <  2:1126633/59‑1 (MQ=255)                                                                                                         tAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                         >  1:367443/1‑55 (MQ=255)                                                                                                         tAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                         <  2:367443/55‑1 (MQ=255)                                                                                                          aaCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                 >  1:931928/1‑126 (MQ=255)                                                                                                          aaCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                 <  2:931928/126‑1 (MQ=255)                                                                                                               tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                                          >  2:398175/1‑48 (MQ=255)                                                                                                               tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                                          <  1:398175/48‑1 (MQ=255)                                                                                                                aCCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                                    <  1:243231/85‑1 (MQ=255)                                                                                                                aCCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                                    >  2:243231/1‑85 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATCAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTc            >  2:397624/1‑137 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTc            <  1:397624/137‑1 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgg          >  1:224699/1‑139 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgg          >  2:1270855/1‑139 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgg          >  1:377237/1‑139 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgg          >  1:374512/1‑139 (MQ=255)                                                                                                                     aTTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCggg         >  2:647075/1‑139 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGcc                                                                                                            <  1:457410/39‑1 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGcc                                                                                                            >  2:457410/1‑39 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                               >  2:947580/1‑52 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg        >  1:1472348/1‑139 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg        >  2:71996/1‑139 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg        <  1:71996/139‑1 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTGAACGGCCACATGGTCCatat                                                                                               <  1:947580/52‑1 (MQ=255)                                                                                                                       tagTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAGAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGga       >  1:968553/3‑139 (MQ=255)                                                                                                                           ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                            >  2:387993/1‑114 (MQ=255)                                                                                                                           ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                            <  1:387993/114‑1 (MQ=255)                                                                                                                            tATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                         <  1:657659/36‑1 (MQ=255)                                                                                                                            tATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                         >  2:657659/1‑36 (MQ=255)                                                                                                                            tATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                     >  2:1397183/1‑104 (MQ=255)                                                                                                                            tATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                     <  1:1397183/104‑1 (MQ=255)                                                                                                                            tATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCa  >  1:510024/1‑139 (MQ=255)                                                                                                                                cccACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATaa                                                        <  2:538141/81‑1 (MQ=255)                                                                                                                                cccACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATaa                                                        >  1:538141/1‑81 (MQ=255)                                                                                                                                    caATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGccc                                                               <  1:826894/70‑1 (MQ=255)                                                                                                                                    caATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGccc                                                               >  2:826894/1‑70 (MQ=255)                                                                                                                                    caATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                 >  2:900283/1‑100 (MQ=255)                                                                                                                                    caATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                 <  1:900283/100‑1 (MQ=255)                                                                                                                                    caATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGa                                   <  2:63425/98‑1 (MQ=255)                                                                                                                                    caATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGa                                   >  1:63425/1‑98 (MQ=255)                                                                                                                                       |                                                                                                                                ACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCA  >  NC_000913/2983154‑2983415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑