Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,007,887	T→C	G126G (GGT→GGC)	ygeX →	2,3‑diaminopropionate ammonia lyase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,007,887	0	T	C	100.0%	224.6 / NA	64	G126G (GGT→GGC)	ygeX	2,3‑diaminopropionate ammonia lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/28);  total (36/28)

GCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAAC  >  NC_000913/3007759‑3008010                                                                                                                                 |                                                                                                                            gCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTggg                                                                                                                   >  1:1273118/1‑139 (MQ=255)      cGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGgcagca                                                                                                              >  1:1329899/1‑139 (MQ=255)        cTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGcagcaca                                                                                                            >  2:694354/1‑139 (MQ=255)        cTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGcagcaca                                                                                                            <  2:1306152/139‑1 (MQ=255)                   tgtgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGAc                                                                                                 >  1:737778/1‑139 (MQ=255)                   tgtgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGAc                                                                                                 >  2:911554/1‑139 (MQ=255)                     tgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAg                                                                                               >  2:275261/1‑139 (MQ=255)                     tgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAg                                                                                               >  2:902258/1‑139 (MQ=255)                     tgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGCCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAg                                                                                               >  2:1111922/1‑139 (MQ=255)                       aaaaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACa                                                                                                >  2:212582/1‑136 (MQ=255)                       aaaaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACa                                                                                                <  1:212582/136‑1 (MQ=255)                       aaaaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGaa                                                                                             >  1:1510784/1‑139 (MQ=255)                         aaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATg                                                                                           <  1:799954/139‑1 (MQ=255)                         aaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCAACTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATg                                                                                           <  1:275261/139‑1 (MQ=255)                               catcTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGa                                                                                     >  1:1396427/1‑139 (MQ=255)                                    tGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTggg                                                                                                                   >  1:568633/1‑104 (MQ=255)                                    tGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTggg                                                                                                                   <  2:568633/104‑1 (MQ=255)                                    tGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTAc                                                                                <  2:1329899/139‑1 (MQ=255)                                      atatCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACAt                                                                              >  1:314992/1‑139 (MQ=255)                                           gAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGa                                                                         <  1:694354/139‑1 (MQ=255)                                                gCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGt                                                                    >  2:1409187/1‑139 (MQ=255)                                                   gTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGc                                                                                          <  1:1286640/114‑1 (MQ=255)                                                   gTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGc                                                                                          >  2:1286640/1‑114 (MQ=255)                                                   gTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   <  2:1235936/137‑1 (MQ=255)                                                   gTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   >  1:1235936/1‑137 (MQ=255)                                                       tttGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTc                                                             >  2:1211847/1‑139 (MQ=255)                                                         tGAGCACCTGAAAAATGCCATCGGCGAAAACATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAg                                                           >  2:1364564/1‑139 (MQ=255)                                                         tGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAg                                                           >  1:495724/1‑139 (MQ=255)                                                          gAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAgg                                                          >  2:47931/1‑139 (MQ=255)                                                           aGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   <  2:1304824/129‑1 (MQ=255)                                                           aGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   >  1:1304824/1‑129 (MQ=255)                                                              aCCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAAcg                                                      >  2:1087277/1‑139 (MQ=255)                                                                cTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAAcgcg                                                    >  1:902080/1‑139 (MQ=255)                                                                cTGAAAAATGCCATCGGCG‑AAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGt                                                   >  1:521885/1‑139 (MQ=255)                                                                 tGAAAAATGCCATCTGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCTGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCTGAAAGGTTCTGCTCAGCAACGCGt                                                   <  2:984920/139‑1 (MQ=255)                                                                     aaaTGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATG‑CGAAAGGTTCTGCTCAGGAACGCGTTGACg                                              <  1:902258/139‑1 (MQ=255)                                                                       aTGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGc                                             <  2:737778/139‑1 (MQ=255)                                                                        tGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGcc                                            >  2:545148/1‑139 (MQ=255)                                                                         gCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCa                                           <  1:1364564/139‑1 (MQ=255)                                                                             tCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   >  1:295763/1‑111 (MQ=255)                                                                             tCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   <  2:295763/111‑1 (MQ=255)                                                                              cGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTg                                      <  1:1409187/139‑1 (MQ=255)                                                                                   aaaaaaTGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCt                                 >  1:274745/1‑139 (MQ=255)                                                                                     aaaaTGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCg                               <  1:911554/139‑1 (MQ=255)                                                                                        aTGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTg                            <  2:314992/139‑1 (MQ=255)                                                                                          gACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACa                                                                                                >  1:1184215/1‑69 (MQ=255)                                                                                          gACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACa                                                                                                <  2:1184215/69‑1 (MQ=255)                                                                                           aCTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCg                                                                                         >  2:1030554/1‑75 (MQ=255)                                                                                           aCTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCg                                                                                         <  1:1030554/75‑1 (MQ=255)                                                                                               tCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTg                     >  1:1556945/1‑139 (MQ=255)                                                                                               tCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTg                     >  2:962070/1‑139 (MQ=255)                                                                                                       accaccGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCa             <  2:1510784/139‑1 (MQ=255)                                                                                                       accaccGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCa             <  2:1596467/139‑1 (MQ=255)                                                                                                         caccGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACg           >  1:1517018/1‑139 (MQ=255)                                                                                                              aCGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGatat      >  1:85034/1‑139 (MQ=255)                                                                                                               cGGCAACCACGGACGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTAGGTGCCGAGTGCATCGTCACGGATATg     <  1:962070/139‑1 (MQ=255)                                                                                                                  cAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAAc  <  1:67373/139‑1 (MQ=255)                                                                                                                  cAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAAc  <  2:988677/139‑1 (MQ=255)                                                                                                                   aaCCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTg                                      >  2:562015/1‑102 (MQ=255)                                                                                                                   aaCCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTg                                      <  1:562015/102‑1 (MQ=255)                                                                                                                        cGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATg                                                                                           >  2:134545/1‑44 (MQ=255)                                                                                                                        cGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATg                                                                                           <  1:134545/44‑1 (MQ=255)                                                                                                                            cgcgGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACa                                                                               <  2:1190191/52‑1 (MQ=255)                                                                                                                            cgcgGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACa                                                                               >  1:1190191/1‑52 (MQ=255)                                                                                                                                 |                                                                                                                            GCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAAC  >  NC_000913/3007759‑3008010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑