Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,075,233	A→G	I317T (ATA→ACA)	yggF ←	fructose 1,6 bisphosphatase isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,075,233	0	A	G	100.0%	196.5 / NA	55	I317T (ATA→ACA)	yggF	fructose 1,6 bisphosphatase isozyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

TGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTC  >  NC_000913/3075101‑3075367                                                                                                                                     |                                                                                                                                       tGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtgttatat                                                                                                                                  >  1:1533574/1‑139 (MQ=255)   gAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtgttatatta                                                                                                                                <  2:802501/139‑1 (MQ=255)   gAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtgttatatta                                                                                                                                <  1:1209555/139‑1 (MQ=255)        agaTGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGt                                                                                                                           >  1:200658/3‑139 (MQ=255)         aaTGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGtt                                                                                                                          >  1:1223589/1‑139 (MQ=255)                tGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCcg                                                                                                                   <  2:1223589/139‑1 (MQ=255)                tGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTCGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCcg                                                                                                                   <  1:797290/139‑1 (MQ=255)                     aGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              <  2:200658/139‑1 (MQ=255)                     aGTTTTCAAGAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              <  1:952185/139‑1 (MQ=255)                       ttttCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCgccgcc                                                                                                            >  2:1474285/1‑139 (MQ=255)                       ttttCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCgccgcc                                                                                                            >  1:1427372/1‑139 (MQ=255)                            aTAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              >  1:1131182/1‑132 (MQ=255)                            aTAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              <  2:1131182/132‑1 (MQ=255)                                 tGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              >  1:1134682/1‑127 (MQ=255)                                 tGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              <  2:1134682/127‑1 (MQ=255)                                      ataaatTGTGCCGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCAATTGGTCTACCAATAGATCACCGTCAAGGCAGCGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTg                                                                                             <  1:1339399/139‑1 (MQ=255)                                              tCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCa                                                                                     >  2:1524113/1‑139 (MQ=255)                                               ccTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCAc                                                                                    <  2:1198950/139‑1 (MQ=255)                                                         cAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGcc                                                                          <  1:50890/139‑1 (MQ=255)                                                             tGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCcgcg                                                                                                                 >  2:795915/1‑96 (MQ=255)                                                             tGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCcgcg                                                                                                                 <  1:795915/96‑1 (MQ=255)                                                                   aGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGa                                                                <  2:878069/139‑1 (MQ=255)                                                                     cacaTCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtgtta                                                                                                                                     >  1:405002/1‑68 (MQ=255)                                                                     cacaTCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtgtta                                                                                                                                     <  2:405002/68‑1 (MQ=255)                                                                     cacaTCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTg                                                                                             <  2:99126/108‑1 (MQ=255)                                                                     cacaTCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTg                                                                                             >  1:99126/1‑108 (MQ=255)                                                                     cacaTCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATa                                                              <  2:1613447/139‑1 (MQ=255)                                                                          catcTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGtt                                                         >  1:151668/1‑139 (MQ=255)                                                                          catcTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGtt                                                         >  2:359664/1‑139 (MQ=255)                                                                                  gTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCt                                                 >  1:1174467/1‑139 (MQ=255)                                                                                       cGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCgccgcc                                                                                                            >  2:1359306/1‑75 (MQ=255)                                                                                       cGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCgccgcc                                                                                                            <  1:1359306/75‑1 (MQ=255)                                                                                       cGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGt                                                                                                     <  1:1307527/82‑1 (MQ=255)                                                                                       cGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGt                                                                                                     >  2:1307527/1‑82 (MQ=255)                                                                                                    cTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCg                               <  1:1474285/139‑1 (MQ=255)                                                                                                    cTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCg                               <  1:359664/139‑1 (MQ=255)                                                                                                         gATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAgcg                          <  2:1427372/139‑1 (MQ=255)                                                                                                             gATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCg                               >  2:879231/1‑130 (MQ=255)                                                                                                             gATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCg                               <  1:879231/130‑1 (MQ=255)                                                                                                             gATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTaa                      <  2:151668/139‑1 (MQ=255)                                                                                                              aTCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              >  1:12710/1‑50 (MQ=255)                                                                                                              aTCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              <  2:12710/50‑1 (MQ=255)                                                                                                               tCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAag                    >  1:1291089/1‑139 (MQ=255)                                                                                                                 aCCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCAcc                                                     <  1:1047115/104‑1 (MQ=255)                                                                                                                 aCCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAagag                  >  1:1337125/1‑139 (MQ=255)                                                                                                                 aCCATCAACGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCAcc                                                     >  2:1047115/1‑104 (MQ=255)                                                                                                                    aTCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGat               <  2:1291089/139‑1 (MQ=255)                                                                                                                     tCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATa                                                              <  2:404609/91‑1 (MQ=255)                                                                                                                     tCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATa                                                              >  1:404609/1‑91 (MQ=255)                                                                                                                             gggAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGtt                                                         <  2:1287717/88‑1 (MQ=255)                                                                                                                             gggAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGtt                                                         >  1:1287717/1‑88 (MQ=255)                                                                                                                             gggAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCg                               >  1:444630/1‑114 (MQ=255)                                                                                                                             gggAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCg                               <  2:444630/114‑1 (MQ=255)                                                                                                                             gggAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTccc      >  1:601273/1‑139 (MQ=255)                                                                                                                             gggAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTccc      >  2:201240/1‑139 (MQ=255)                                                                                                                                aGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGt                                                                        <  1:461884/70‑1 (MQ=255)                                                                                                                                aGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGt                                                                        >  2:461884/1‑70 (MQ=255)                                                                                                                                 gTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCAc                                                                                    >  1:604426/1‑57 (MQ=255)                                                                                                                                 gTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCAc                                                                                    <  2:604426/57‑1 (MQ=255)                                                                                                                                 gTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTc  >  1:817496/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       TGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTC  >  NC_000913/3075101‑3075367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑