Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,308	G→A	S121L (TCG→TTG)	ftsP ←	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,308	0	G	A	100.0%	202.2 / NA	63	S121L (TCG→TTG)	ftsP	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/38);  total (25/38)

CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGT  >  NC_000913/3162177‑3162438                                                                                                                                    |                                                                                                                                    cTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcatcat                                                                                                                              <  2:699936/139‑1 (MQ=255)         aGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCg                                                                                                                     <  2:213156/139‑1 (MQ=255)           ccACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGa                                                                                                                   <  1:740322/139‑1 (MQ=255)           ccACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGa                                                                                                                   >  1:451566/1‑139 (MQ=255)                     gCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCa                                                                                                         >  2:1262392/1‑139 (MQ=255)                       aaGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGc                                                                                                       <  2:292316/139‑1 (MQ=255)                        aGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCg                                                                                                      <  1:1127015/139‑1 (MQ=255)                        aGGCCGTTATAGACCTGCTCAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCTATCAGCg                                                                                                      <  1:1054715/139‑1 (MQ=255)                            cGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCCGTGCCGGACCGCCCATCAGCGGGc                                                                                                   <  2:1317780/139‑1 (MQ=255)                               tataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               <  2:481590/139‑1 (MQ=255)                               tataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               <  2:233742/139‑1 (MQ=255)                                        gctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAg                                                                                      >  2:1112180/1‑139 (MQ=255)                                        gctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAg                                                                                      >  1:1511301/1‑139 (MQ=255)                                         ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc                                                                                     >  2:923191/1‑139 (MQ=255)                                              ccGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGc                                                                                >  2:677598/1‑139 (MQ=255)                                              ccGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGc                                                                                <  1:677598/139‑1 (MQ=255)                                                   cGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCg                                                                           <  2:837914/139‑1 (MQ=255)                                                        gggAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCAtt                                                                      >  2:1372650/1‑139 (MQ=255)                                                           aGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTgag                                                                   >  2:846524/1‑139 (MQ=255)                                                                     tGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTg                                                         >  1:254472/1‑139 (MQ=255)                                                                           cacaGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCt                                                          >  1:1533851/1‑132 (MQ=255)                                                                           cacaGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCt                                                          <  2:1533851/132‑1 (MQ=255)                                                                              agagTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                <  2:92160/139‑1 (MQ=255)                                                                                agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTg                                              <  2:38160/139‑1 (MQ=255)                                                                                agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTg                                              <  2:451566/139‑1 (MQ=255)                                                                                agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTg                                              >  1:732221/1‑139 (MQ=255)                                                                                 gTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                <  2:1434787/136‑1 (MQ=255)                                                                                 gTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                >  1:1434787/1‑136 (MQ=255)                                                                                  tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt                                            >  2:1538399/1‑139 (MQ=255)                                                                                  tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt                                            <  1:1262392/139‑1 (MQ=255)                                                                                  tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTCTCTGTCAGGCGGTTGCt                                            <  1:923191/139‑1 (MQ=255)                                                                                     cTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGt                                                        >  1:1291631/1‑124 (MQ=255)                                                                                     cTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGt                                                        <  2:1291631/124‑1 (MQ=255)                                                                                      tGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTaa                                        <  1:207730/139‑1 (MQ=255)                                                                                        cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGcc                                                                                    <  1:753169/93‑1 (MQ=255)                                                                                        cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGcc                                                                                    >  2:753169/1‑93 (MQ=255)                                                                                        cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGt                                                        >  2:1177386/1‑121 (MQ=255)                                                                                        cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGt                                                        <  1:1177386/121‑1 (MQ=255)                                                                                        cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAAt                                      <  2:254472/139‑1 (MQ=255)                                                                                        cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAAt                                      <  2:1511301/139‑1 (MQ=255)                                                                                        cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGGGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAAt                                      <  1:533517/139‑1 (MQ=255)                                                                                          ttCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATaa                                    >  1:563511/1‑139 (MQ=255)                                                                                            cTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCggg                                                                                                    <  1:1520129/73‑1 (MQ=255)                                                                                            cTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCggg                                                                                                    >  2:1520129/1‑73 (MQ=255)                                                                                            cTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGc                                  >  2:1180274/1‑139 (MQ=255)                                                                                             tGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCt                                 >  1:65419/1‑139 (MQ=255)                                                                                               gCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTa                               <  2:1218561/139‑1 (MQ=255)                                                                                               gCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTa                               <  1:1180274/139‑1 (MQ=255)                                                                                                        cAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACAtcgtcg                      <  2:732221/139‑1 (MQ=255)                                                                                                         aGTACGGGTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGc                     <  2:65419/139‑1 (MQ=255)                                                                                                              gggTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTc                <  1:1538399/139‑1 (MQ=255)                                                                                                               ggTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCt                                                                                                 <  2:417316/57‑1 (MQ=255)                                                                                                               ggTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCt                                                                                                 >  1:417316/1‑57 (MQ=255)                                                                                                                gTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGc                                                                                <  2:1379790/73‑1 (MQ=255)                                                                                                                gTGCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGc                                                                                >  1:1379790/1‑73 (MQ=255)                                                                                                                  gCCCAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGa            <  1:164381/139‑1 (MQ=255)                                                                                                                     cAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGcc                                                                                                  >  1:1076052/1‑50 (MQ=255)                                                                                                                     cAGTCAGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGcc                                                                                                  <  2:1076052/50‑1 (MQ=255)                                                                                                                          aGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTgaga                                                                  <  1:224551/77‑1 (MQ=255)                                                                                                                          aGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTgaga                                                                  >  2:224551/1‑77 (MQ=255)                                                                                                                          aGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCgg                                                 <  2:1204333/94‑1 (MQ=255)                                                                                                                          aGCGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCgg                                                 >  1:1204333/1‑94 (MQ=255)                                                                                                                            cGTTTGGCAACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGt  <  2:1205718/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCAT‑CCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGT  >  NC_000913/3162177‑3162438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑