Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,271,932	T→C	T274A (ACG→GCG)	garR ←	tartronate semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,271,932	0	T	C	100.0%	215.2 / NA	59	T274A (ACG→GCG)	garR	tartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/35);  total (24/35)

AGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATG  >  NC_000913/3271799‑3272046                                                                                                                                      |                                                                                                                   aGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTa                                                                                                               >  1:187633/1‑139 (MQ=255)        ttCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATc                                                                                                        >  2:1285111/1‑139 (MQ=255)              tATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCg                                                                                                  <  1:340266/139‑1 (MQ=255)                           ttCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                     <  1:1285111/139‑1 (MQ=255)                           ttCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                     <  2:808700/139‑1 (MQ=255)                           ttCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                     <  2:187633/139‑1 (MQ=255)                           ttCCAGGCCCGCAGTGCAATGCTCCATCCGGGCACGACGTCATTAACGATTAACTTCGTCTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                     <  1:283568/139‑1 (MQ=255)                             ccAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatcat                                                                                   <  2:525536/139‑1 (MQ=255)                              cAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCCACTTTCGCCAGTTTTTCGTAGTAGCACGCCACGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCatcatc                                                                                  >  1:273661/1‑139 (MQ=255)                               aGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCAtct                                                                                 >  2:655826/1‑139 (MQ=255)                                   ccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCAt                                                                             <  2:1215054/139‑1 (MQ=255)                                           cAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                     >  1:320192/1‑123 (MQ=255)                                           cAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                     <  2:320192/123‑1 (MQ=255)                                                          gCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGtt                                                      >  2:1018618/1‑139 (MQ=255)                                                               acgTCATTGACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTgcg                                                 <  1:1167314/139‑1 (MQ=255)                                                               acgTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                     >  1:647739/1‑103 (MQ=255)                                                               acgTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                     <  2:647739/103‑1 (MQ=255)                                                                cgTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTgcgc                                                >  2:1438219/1‑139 (MQ=255)                                                                   cATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGa                                             >  1:92584/1‑139 (MQ=255)                                                                    aTTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGAc                                            >  2:19185/1‑139 (MQ=255)                                                                    aTTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGAc                                            <  2:92584/139‑1 (MQ=255)                                                                     ttAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACg                                           <  2:843750/139‑1 (MQ=255)                                                                         cGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                       <  1:319981/139‑1 (MQ=255)                                                                         cGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                       <  1:729045/139‑1 (MQ=255)                                                                             tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCt                                                                                                       >  2:1461910/1‑71 (MQ=255)                                                                             tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCt                                                                                                       <  1:1461910/71‑1 (MQ=255)                                                                             tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                       <  2:811315/135‑1 (MQ=255)                                                                             tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                       >  1:811315/1‑135 (MQ=255)                                                                             tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTgaga                                   >  2:807335/1‑139 (MQ=255)                                                                              aaCTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGaa                                  <  2:324399/139‑1 (MQ=255)                                                                              aaCTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGaa                                  <  1:663882/139‑1 (MQ=255)                                                                               aCTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                           >  2:612707/1‑113 (MQ=255)                                                                               aCTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                           <  1:612707/113‑1 (MQ=255)                                                                                     aCTTTCGCCAGTTTTTCTTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGca                                                                                       <  1:327629/79‑1 (MQ=255)                                                                                     aCTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGca                                                                                       >  2:327629/1‑79 (MQ=255)                                                                                     aCTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCa                           >  1:61525/1‑139 (MQ=255)                                                                                     aCTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCa                           <  1:1608717/139‑1 (MQ=255)                                                                                         tCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAgcgc                       >  1:962921/1‑139 (MQ=255)                                                                                               gTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGc                 <  2:1139897/139‑1 (MQ=255)                                                                                               gTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGc                 <  2:1608468/139‑1 (MQ=255)                                                                                               gTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGc                 <  1:19185/139‑1 (MQ=255)                                                                                                tttttCGTAGTAGCACGGCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCAGCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGcc                >  1:924377/1‑139 (MQ=255)                                                                                                tttttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGcc                <  1:1018618/139‑1 (MQ=255)                                                                                                 ttttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCa               <  1:1428559/139‑1 (MQ=255)                                                                                                        agtagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCtt        <  1:1438219/139‑1 (MQ=255)                                                                                                         gtagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTa       <  2:154239/139‑1 (MQ=255)                                                                                                          tagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCa                                                                                                <  2:937249/49‑1 (MQ=255)                                                                                                          tagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCa                                                                                                >  1:937249/1‑49 (MQ=255)                                                                                                              aCGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg  <  1:655826/139‑1 (MQ=255)                                                                                                               cGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCa                      <  2:1407103/118‑1 (MQ=255)                                                                                                               cGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCa                      >  1:1407103/1‑118 (MQ=255)                                                                                                               cGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt            >  2:1046876/1‑128 (MQ=255)                                                                                                               cGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt            <  1:1046876/128‑1 (MQ=255)                                                                                                                gCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg  <  2:1355457/137‑1 (MQ=255)                                                                                                                gCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg  >  1:1355457/1‑137 (MQ=255)                                                                                                                    gggCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCa               >  2:708181/1‑120 (MQ=255)                                                                                                                    gggCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCa               <  1:708181/120‑1 (MQ=255)                                                                                                                               tCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCa               >  2:254952/1‑109 (MQ=255)                                                                                                                               tCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCa               <  1:254952/109‑1 (MQ=255)                                                                                                                                      |                                                                                                                   AGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATG  >  NC_000913/3271799‑3272046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑