Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,301,599	G→A	G39S (GGC→AGC)	yhbU →	U32 peptidase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,301,599	0	G	A	100.0%	114.1 / NA	36	G39S (GGC→AGC)	yhbU	U32 peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/16);  total (20/16)

ATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATATG  >  NC_000913/3301482‑3301733                                                                                                                      |                                                                                                                                       aTTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGaaaa                                                                                                                   <  2:1139886/139‑1 (MQ=255) aTTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGaaaa                                                                                                                   >  2:1047988/1‑139 (MQ=255)            gctCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGc                                                                                                                                      <  1:989361/109‑1 (MQ=255)                tgccctgccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGACGATACCAATGCTCGTCACTTCGCCAGCCTTAATTTTACCGAGAAAAAATTGCAGGAAGCgg                                                                                                    <  1:1042434/139‑1 (MQ=255)                                 cccGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCaa                                                                                   >  2:1543933/1‑139 (MQ=255)                                            aGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAgg                                                                                                          >  2:931139/1‑105 (MQ=255)                                            aGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAgg                                                                                                          <  1:931139/105‑1 (MQ=255)                                                ggcCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCg                                                                                                                         >  2:550861/1‑86 (MQ=255)                                                ggcCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCg                                                                                                                         <  1:550861/86‑1 (MQ=255)                                                ggcCATCGAAAACGGAGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACtt                                                                    >  1:1074859/1‑139 (MQ=255)                                                  ccATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTca                                                                  <  2:1074859/139‑1 (MQ=255)                                                     tCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACt                                                                     >  1:889038/1‑133 (MQ=255)                                                     tCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACt                                                                     <  2:889038/133‑1 (MQ=255)                                                     tCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACAt                                                               <  1:699807/139‑1 (MQ=255)                                                            cGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGc                                                                                                                                      <  1:912703/61‑1 (MQ=255)                                                            cGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGc                                                                                                                                      >  2:912703/1‑61 (MQ=255)                                                                               atCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGc                                                                                                             <  1:1578354/67‑1 (MQ=255)                                                                               atCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGc                                                                                                             >  2:1578354/1‑67 (MQ=255)                                                                                    gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCg                                      <  1:135830/133‑1 (MQ=255)                                                                                    gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCg                                      >  2:135830/1‑133 (MQ=255)                                                                                    gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGt                                >  2:681724/1‑139 (MQ=255)                                                                                    gCTAAAAGATGATACCAATGCCC‑‑CACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTg                                              <  2:1382141/123‑1 (MQ=255)                                                                                    gCTAAAAGATGATACCAATGCCC‑‑CACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTg                                              >  1:1382141/1‑123 (MQ=255)                                                                                              gatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGtt                      >  2:947259/1‑139 (MQ=255)                                                                                                   cAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAgg                                                                                                          <  2:524459/50‑1 (MQ=255)                                                                                                   cAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAgg                                                                                                          >  1:524459/1‑50 (MQ=255)                                                                                                   cAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAg                 <  1:681724/139‑1 (MQ=255)                                                                                                     aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcg               <  1:1543933/139‑1 (MQ=255)                                                                                                      tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcgc              >  2:1275329/1‑139 (MQ=255)                                                                                                      tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcgc              >  1:143755/1‑139 (MQ=255)                                                                                                           gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAACCTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGt         >  1:1152125/1‑139 (MQ=255)                                                                                                            tCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTg        >  2:662477/1‑139 (MQ=255)                                                                                                                  cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa                                                                                                        <  2:1141613/37‑1 (MQ=255)                                                                                                                  cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa                                                                                                        >  1:1141613/1‑37 (MQ=255)                                                                                                                  cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATATg  >  2:1572480/1‑139 (MQ=255)                                                                                                                  cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATATg  >  2:1587630/1‑139 (MQ=255)                                                                                                                      |                                                                                                                                       ATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATATG  >  NC_000913/3301482‑3301733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑