| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,372,254 | G→A | G107G (GGC→GGT) | nanT ← | sialic acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,372,254 | 0 | G | A | 93.9% | 95.4 / 1.9 | 33 | G107G (GGC→GGT) | nanT | sialic acid transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (17/14); minor base C (1/1); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01 | |||||||||||
TGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGA > NC_000913/3372122‑3372374 | tGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGgc > 1:1358277/1‑139 (MQ=255) aCTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGcc < 2:567686/139‑1 (MQ=255) gCTTTGTTACGCAGATGTGTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCg > 1:895675/1‑139 (MQ=255) gTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCg > 2:113037/1‑139 (MQ=255) aCGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGcc < 1:907357/139‑1 (MQ=255) ttGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTg > 1:1308432/1‑99 (MQ=255) ttGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTg < 2:1308432/99‑1 (MQ=255) cAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCg < 2:137806/105‑1 (MQ=255) cAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCg > 1:137806/1‑105 (MQ=255) cAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg > 2:81127/1‑138 (MQ=255) cAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg < 1:81127/138‑1 (MQ=255) cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCaa > 1:309645/1‑97 (MQ=255) cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCaa < 2:309645/97‑1 (MQ=255) cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTgg > 1:650482/1‑139 (MQ=255) cAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGACAAGAGAACGATGCTGGTGAc < 2:895675/139‑1 (MQ=255) ggCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAgacga > 2:523085/1‑139 (MQ=255) ggCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAgacga > 2:1485558/1‑139 (MQ=255) gcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGc > 1:753662/1‑139 (MQ=255) cTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAc > 2:542235/1‑123 (MQ=255) cTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAc < 1:542235/123‑1 (MQ=255) ttCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATTCTGGTGACCATTGCCAGACGACGc < 2:1069683/127‑1 (MQ=255) ttCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGc > 1:1069683/1‑127 (MQ=255) cGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATa < 2:650482/139‑1 (MQ=255) ccATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTg < 2:1182580/98‑1 (MQ=255) ccATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTg > 1:1182580/1‑98 (MQ=255) ccATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAgc > 1:70044/1‑139 (MQ=255) tCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGcc < 2:753662/139‑1 (MQ=255) cAGACGAGCGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGccgccg > 1:648435/1‑139 (MQ=255) cGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagagaa > 1:835631/1‑63 (MQ=255) cGATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagagaa < 2:835631/63‑1 (MQ=255) gATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAg < 2:1384525/97‑1 (MQ=255) gATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAg > 1:1384525/1‑97 (MQ=255) gATAAACATGGTGATGT‑AACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCga < 2:1358277/139‑1 (MQ=255) | TGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGT‑AGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGA > NC_000913/3372122‑3372374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |