Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,440,950	G→A	T27I (ACC→ATC)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,440,950	0	G	A	100.0%	241.8 / NA	74	T27I (ACC→ATC)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/36);  total (38/36)

TACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGT  >  NC_000913/3440827‑3441085                                                                                                                            |                                                                                                                                        tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGTTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                          <  2:858270/139‑1 (MQ=255) tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                          <  2:997181/139‑1 (MQ=255) tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                          >  1:574404/1‑139 (MQ=255)     cATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGa                                                                                                                      >  1:536161/1‑139 (MQ=255)           gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                                >  1:51785/1‑139 (MQ=255)           gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                                >  1:737871/1‑139 (MQ=255)           gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                                >  2:47980/1‑139 (MQ=255)                ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  2:1366101/139‑1 (MQ=255)                ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  1:425077/139‑1 (MQ=255)                ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  2:51785/139‑1 (MQ=255)                 cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  1:658658/138‑1 (MQ=255)                 cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           >  2:658658/1‑138 (MQ=255)                 cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                                          >  1:732352/1‑139 (MQ=255)                   tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGa                                                                                                        >  1:756638/1‑139 (MQ=255)                    cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGat                                                                                                       >  1:1383073/1‑139 (MQ=255)                     aaTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGata                                                                                                      <  1:47980/139‑1 (MQ=255)                      aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAATCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  2:1088600/133‑1 (MQ=255)                      aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           >  1:1088600/1‑133 (MQ=255)                      aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                                     >  2:1067404/1‑139 (MQ=255)                             ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  2:684883/126‑1 (MQ=255)                             ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           >  1:684883/1‑126 (MQ=255)                             ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAg                                                                                              >  2:781740/1‑139 (MQ=255)                             ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAg                                                                                              >  1:1504141/1‑139 (MQ=255)                                cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                           <  2:1504141/139‑1 (MQ=255)                                cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                           <  1:1056537/139‑1 (MQ=255)                                 ggTCACCGCGCAACCCTGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                                          >  2:677118/1‑139 (MQ=255)                                 ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                                          >  1:1311715/1‑139 (MQ=255)                                 ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                                          >  2:1450498/1‑139 (MQ=255)                                 ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAACCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                                          >  1:79071/1‑139 (MQ=255)                                        gcgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTa                                                                                   >  1:1048904/1‑139 (MQ=255)                                              cccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  1:1379419/109‑1 (MQ=255)                                              cccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           >  2:1379419/1‑109 (MQ=255)                                               ccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                                            <  1:371188/139‑1 (MQ=255)                                                 ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                                          <  2:756638/139‑1 (MQ=255)                                                        aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACt                                                                                                                   >  1:1184075/1‑91 (MQ=255)                                                        aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACt                                                                                                                   <  2:1184075/91‑1 (MQ=255)                                                           agagCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAcc                                                                <  1:628507/139‑1 (MQ=255)                                                               cAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGc                                                            <  2:536161/139‑1 (MQ=255)                                                               cAGAATACGGCCCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGc                                                            <  2:1048904/139‑1 (MQ=255)                                                                  aaTACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                                         <  2:1383073/139‑1 (MQ=255)                                                                    tACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgt                                                       <  2:1323167/139‑1 (MQ=255)                                                                       ggCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                                    >  1:687890/1‑139 (MQ=255)                                                                         cgcAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                                  <  2:737871/139‑1 (MQ=255)                                                                              tGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                                         >  2:1602960/1‑127 (MQ=255)                                                                              tGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                                         <  1:1602960/127‑1 (MQ=255)                                                                              tGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                             <  1:677118/139‑1 (MQ=255)                                                                                     cccAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACt                                      <  2:79071/139‑1 (MQ=255)                                                                                          agTATGGCCAAAGCTACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           >  2:979076/1‑65 (MQ=255)                                                                                          agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  1:979076/65‑1 (MQ=255)                                                                                               ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           >  1:245360/1‑60 (MQ=255)                                                                                               ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  2:245360/60‑1 (MQ=255)                                                                                               ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt                            >  1:122435/1‑139 (MQ=255)                                                                                               ggCCAAAGCCACGCTCTAAAGGCTCAAGCATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt                            >  1:341327/1‑139 (MQ=255)                                                                                                    aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  1:517302/55‑1 (MQ=255)                                                                                                    aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           >  2:517302/1‑55 (MQ=255)                                                                                                    aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAc                                                                 <  1:682446/97‑1 (MQ=255)                                                                                                    aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAc                                                                 >  2:682446/1‑97 (MQ=255)                                                                                                    aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTcc                                                   <  2:1291921/111‑1 (MQ=255)                                                                                                    aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTcc                                                   >  1:1291921/1‑111 (MQ=255)                                                                                                      gCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgtgcgt                                                                                                                         >  2:442056/1‑39 (MQ=255)                                                                                                      gCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgtgcgt                                                                                                                         <  1:442056/39‑1 (MQ=255)                                                                                                      gCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTa                     >  1:1427362/1‑139 (MQ=255)                                                                                                      gCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTa                     >  1:749091/1‑139 (MQ=255)                                                                                                                   ggCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATc        <  2:884177/139‑1 (MQ=255)                                                                                                                   ggCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATc        <  2:732352/139‑1 (MQ=255)                                                                                                                      tCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAgg     <  2:1311715/139‑1 (MQ=255)                                                                                                                       cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           <  1:537346/36‑1 (MQ=255)                                                                                                                       cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                           >  2:537346/1‑36 (MQ=255)                                                                                                                       cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAGCTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgt    >  1:933809/1‑139 (MQ=255)                                                                                                                       cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgt    <  2:749091/139‑1 (MQ=255)                                                                                                                       cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgt    >  2:1336331/1‑139 (MQ=255)                                                                                                                         aGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                     >  1:1303183/1‑72 (MQ=255)                                                                                                                         aGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCtta              <  1:368363/127‑3 (MQ=255)                                                                                                                         aGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgtgt  >  2:1560727/1‑139 (MQ=255)                                                                                                                            |                                                                                                                                        TACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGT  >  NC_000913/3440827‑3441085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑