Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,074	C→T	*257* (TAG→TAA)	bioH ←	pimeloyl‑ACP methyl ester carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,074	0	C	T	100.0%	228.7 / NA	71	*257* (TAG→TAA)	bioH	pimeloyl‑ACP methyl ester carboxylesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/37);  total (34/37)

AGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCAT  >  NC_000913/3543940‑3544196                                                                                                                                       |                                                                                                                           aGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTaca                                                                                                                        <  2:585732/139‑1 (MQ=255)      tGCATATGGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCctc                                                                                                                   >  1:1311568/1‑139 (MQ=255)      tGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCctc                                                                                                                   >  2:1179697/1‑139 (MQ=255)      tGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCctc                                                                                                                   >  1:330342/1‑139 (MQ=255)      tGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCctc                                                                                                                   >  1:1230690/1‑139 (MQ=255)            taGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTc                                                                                                             <  1:1179697/139‑1 (MQ=255)                  aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCctc                                                                                                                   <  2:156766/127‑1 (MQ=255)                  aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCctc                                                                                                                   >  1:156766/1‑127 (MQ=255)                    ataatGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGccac                                                                                                     <  1:1111017/139‑1 (MQ=255)                    ataatGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGccac                                                                                                     <  1:1600604/139‑1 (MQ=255)                         gCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACcagca                                                                                                <  1:323331/139‑1 (MQ=255)                          cTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCctct                                                                                                                  <  1:763378/120‑1 (MQ=255)                          cTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCctct                                                                                                                  >  2:763378/1‑120 (MQ=255)                          cTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACcagcag                                                                                               <  2:1230690/139‑1 (MQ=255)                                    gAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTa                                                                                                                          >  2:882006/1‑102 (MQ=255)                                          ctctTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGcc                                                                               >  1:849189/1‑139 (MQ=255)                                            ctTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTaca                                                                                                                        >  1:1507230/1‑96 (MQ=255)                                            ctTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTaca                                                                                                                        <  2:1507230/96‑1 (MQ=255)                                               gCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATg                                                                          >  2:352563/1‑139 (MQ=255)                                                 aGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCg                                                                        <  2:58271/139‑1 (MQ=255)                                                     atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGcc                                                                               <  1:168407/128‑1 (MQ=255)                                                     atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGcc                                                                               >  2:168407/1‑128 (MQ=255)                                                         tGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGcc                                                                               >  1:601954/1‑124 (MQ=255)                                                         tGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGcc                                                                               <  2:601954/124‑1 (MQ=255)                                                             cTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACa                                                                                         <  1:117199/110‑1 (MQ=255)                                                             cTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACa                                                                                         >  2:117199/1‑110 (MQ=255)                                                               ttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCtt                                                                                                              >  1:1262675/1‑87 (MQ=255)                                                               ttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCtt                                                                                                              <  2:1262675/87‑1 (MQ=255)                                                                tACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCAt                                                         >  2:628192/1‑139 (MQ=255)                                                                tACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCAt                                                         >  1:1144547/1‑139 (MQ=255)                                                                       cTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACAcc                                                                                                                      <  1:934583/71‑1 (MQ=255)                                                                       cTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACAcc                                                                                                                      >  2:934583/1‑71 (MQ=255)                                                                       cTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaataaa                                                                 <  1:1408424/124‑1 (MQ=255)                                                                       cTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaataaa                                                                 >  2:1408424/1‑124 (MQ=255)                                                                        tGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCt                                                 <  2:849189/139‑1 (MQ=255)                                                                          tCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCttt                                               >  2:1464901/1‑139 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                           <  1:628192/139‑1 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                           <  1:1464901/139‑1 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                           <  2:1304833/139‑1 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                           <  1:352563/139‑1 (MQ=255)                                                                               agaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                          <  1:1019380/139‑1 (MQ=255)                                                                               agaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                          <  2:62535/139‑1 (MQ=255)                                                                               agaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                          <  2:1144547/139‑1 (MQ=255)                                                                                gaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGaa                                         <  2:1165090/139‑1 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                           >  2:1200228/1‑133 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                           >  1:313106/1‑133 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                           <  2:313106/133‑1 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                          >  2:1392680/1‑134 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                          <  1:1392680/134‑1 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGata                                     >  1:462558/1‑139 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGata                                     >  2:523828/1‑139 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAAGTAACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                           <  1:1200228/133‑1 (MQ=255)                                                                                     gTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGatat                                    <  2:1498505/139‑1 (MQ=255)                                                                                        ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACAcc                                                                                                                      <  2:1568236/54‑1 (MQ=255)                                                                                        ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACAcc                                                                                                                      >  1:1568236/1‑54 (MQ=255)                                                                                        ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGc                                                                         <  1:902334/99‑1 (MQ=255)                                                                                        ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGc                                                                         >  2:902334/1‑99 (MQ=255)                                                                                           tGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGc                                                                                <  2:546292/89‑1 (MQ=255)                                                                                           tGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGc                                                                                >  1:546292/1‑89 (MQ=255)                                                                                             cggcgAGTCAGTACAGTTCTGTCTCGCCGTTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCg                            >  1:1229033/1‑139 (MQ=255)                                                                                              ggcgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                          <  2:217287/124‑1 (MQ=255)                                                                                              ggcgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                          >  1:217287/1‑124 (MQ=255)                                                                                                             ttCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                           >  2:876604/1‑108 (MQ=255)                                                                                                             ttCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                           <  1:876604/108‑1 (MQ=255)                                                                                                             ttCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGtt            >  2:513102/1‑139 (MQ=255)                                                                                                                  tctcGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATggg                                                      >  2:610949/1‑92 (MQ=255)                                                                                                                  tctcGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATggg                                                      <  1:610949/92‑1 (MQ=255)                                                                                                                      gCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCa   >  1:323027/1‑139 (MQ=255)                                                                                                                       ccATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCAt  <  1:513102/139‑1 (MQ=255)                                                                                                                                aaGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTgg                                             <  1:1525690/87‑1 (MQ=255)                                                                                                                                aaGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTgg                                             >  2:1525690/1‑87 (MQ=255)                                                                                                                                       |                                                                                                                           AGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCAT  >  NC_000913/3543940‑3544196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑