| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,556,033 | A→G | Y273H (TAC→CAC) | rtcA ← | RNA 3'‑terminal phosphate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,556,033 | 0 | A | G | 100.0% | 180.5 / NA | 51 | Y273H (TAC→CAC) | rtcA | RNA 3'‑terminal phosphate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/28); total (23/28) | |||||||||||
CTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACC > NC_000913/3555899‑3556153 | cTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGt > 1:1270248/1‑139 (MQ=255) caccacCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTc < 2:149322/139‑1 (MQ=255) accaccGCGATATTGGTCAGCAGATGGCATGAGGGTTGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCa < 1:789451/139‑1 (MQ=255) accaccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCa < 2:1270248/139‑1 (MQ=255) caccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCAc < 1:1535826/139‑1 (MQ=255) accGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAACAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct < 1:1440259/139‑1 (MQ=255) accACGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct < 2:1046390/139‑1 (MQ=255) gcgATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCtt > 2:1498836/1‑139 (MQ=255) tGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCaa < 2:1570143/139‑1 (MQ=255) ggCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAac > 1:174861/1‑139 (MQ=255) aTGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCaa < 2:1597452/117‑1 (MQ=255) aTGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCaa > 1:1597452/1‑117 (MQ=255) gggCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCAGCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACt > 2:1011703/1‑139 (MQ=255) gggCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACt > 2:1024169/1‑139 (MQ=255) gggCGACCGTAAATTCCCCCGCGCCCACCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACt > 1:1148267/1‑139 (MQ=255) ggCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTg < 2:1528148/139‑1 (MQ=255) gACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg < 1:265344/139‑1 (MQ=255) gACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg < 1:1296368/139‑1 (MQ=255) cccccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGGCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCAcc < 1:66010/139‑1 (MQ=255) ccccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCAcc < 1:381318/138‑1 (MQ=255) ccccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCAcc > 2:381318/1‑138 (MQ=255) cgcgCCCACCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACcgacg < 2:1148267/139‑1 (MQ=255) gccAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACaaaaa > 1:451955/1‑139 (MQ=255) gcgcCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCg < 1:1498836/139‑1 (MQ=255) ccATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTc < 2:174861/139‑1 (MQ=255) tCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGt < 1:1501746/139‑1 (MQ=255) cACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATAttt > 1:312072/1‑139 (MQ=255) gTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCAc < 1:1245883/55‑1 (MQ=255) gTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCAc > 2:1245883/1‑55 (MQ=255) gTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATAtttt < 1:1078907/135‑1 (MQ=255) gTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATAtttt > 2:1078907/1‑135 (MQ=255) ggTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTc < 1:278179/139‑1 (MQ=255) tCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCa < 2:124476/49‑1 (MQ=255) tCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCa > 1:124476/1‑49 (MQ=255) atT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCa < 2:949618/39‑1 (MQ=255) atT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCa > 1:949618/1‑39 (MQ=255) atT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCga < 1:255289/139‑1 (MQ=255) atT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCga > 1:139205/1‑139 (MQ=255) t‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACtgtg > 2:1523303/1‑55 (MQ=255) t‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACtgtg < 1:1523303/55‑1 (MQ=255) t‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAg > 2:1288319/1‑134 (MQ=255) t‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAg < 1:1288319/134‑1 (MQ=255) t‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCgaga < 2:139205/139‑1 (MQ=255) ccccACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACg > 2:532348/1‑139 (MQ=255) ccccACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACg > 2:783919/1‑139 (MQ=255) cACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCAcc > 2:1307189/1‑43 (MQ=255) cACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCAcc < 1:1307189/43‑1 (MQ=255) cACCGCCGCCGTGCGTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACTCGCTTTTCACCGACGACAAAAAAGCGCTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTa > 2:978538/1‑139 (MQ=255) ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTAcc > 2:1449728/1‑139 (MQ=255) gccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCg < 1:161414/92‑1 (MQ=255) gccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCg > 2:161414/1‑92 (MQ=255) | CTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACC > NC_000913/3555899‑3556153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |